LOCUS       sequence-311344-        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  20 repeats of Pecf11 sponge.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: pXWS20Pecf11
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      1797..1868
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1884..1911
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atcgatttta gagctgccgc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc
       61 ccgctacgta acacctctgt actcagctta tctcgggcct ccacaccgct cgtcacatcc
      121 tgtgatccac tcttcatccc gctacgtaac acctctctta ggtgtcccta gaatgagtat
      181 cgattttaga gctgccgcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc
      241 gctacgtaac acctctgtac tcagcttatc tcgggcctcc acaccgctcg tcacatcctg
      301 tgatccactc ttcatcccgc tacgtaacac ctctcttagg tgtccctaga atttccatcg
      361 attttagagc tgccgcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc
      421 tacgtaacac ctctgtactc agcttatctc gggcctccac accgctcgtc acatcctgtg
      481 atccactctt catcccgcta cgtaacacct ctcttaggtg tccctagaat gactatcgat
      541 tttagagctg ccgcctccac accgctcgtc acatcctgtg atccactctt catcccgcta
      601 cgtaacacct ctgtactcag cttatctcgg gcctccacac cgctcgtcac atcctgtgat
      661 ccactcttca tcccgctacg taacacctct cttaggtgtc cctagaattg atatcgattt
      721 tagagctgcc gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg
      781 taacacctct gtactcagct tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc
      841 actcttcatc ccgctacgta acacctctct taggtgtccc tagaattact agagatcgat
      901 tttagagctg ccgcctccac accgctcgtc acatcctgtg atccactctt catcccgcta
      961 cgtaacacct ctgtactcag cttatctcgg gcctccacac cgctcgtcac atcctgtgat
     1021 ccactcttca tcccgctacg taacacctct cttaggtgtc cctagaatga gtatcgattt
     1081 tagagctgcc gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg
     1141 taacacctct gtactcagct tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc
     1201 actcttcatc ccgctacgta acacctctct taggtgtccc tagaatttcc atcgatttta
     1261 gagctgccgc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc ccgctacgta
     1321 acacctctgt actcagctta tctcgggcct ccacaccgct cgtcacatcc tgtgatccac
     1381 tcttcatccc gctacgtaac acctctctta ggtgtcccta gaatgactat cgattttaga
     1441 gctgccgcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc gctacgtaac
     1501 acctctgtac tcagcttatc tcgggcctcc acaccgctcg tcacatcctg tgatccactc
     1561 ttcatcccgc tacgtaacac ctctcttagg tgtccctaga attgatatcg attttagagc
     1621 tgccgcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc tacgtaacac
     1681 ctctgtactc agcttatctc gggcctccac accgctcgtc acatcctgtg atccactctt
     1741 catcccgcta cgtaacacct ctcttaggtg tccctagaat tactagagcc aggcatcaaa
     1801 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1861 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//
