LOCUS       sequence-311345-        3705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  40 repeats of Pecf11 sponge.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: pXWS40Pecf11
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      3585..3656
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3672..3699
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atcgatttta gagctgccgc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc
       61 ccgctacgta acacctctgt actcagctta tctcgggcct ccacaccgct cgtcacatcc
      121 tgtgatccac tcttcatccc gctacgtaac acctctctta ggtgtcccta gaatgagtat
      181 cgattttaga gctgccgcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc
      241 gctacgtaac acctctgtac tcagcttatc tcgggcctcc acaccgctcg tcacatcctg
      301 tgatccactc ttcatcccgc tacgtaacac ctctcttagg tgtccctaga atttccatcg
      361 attttagagc tgccgcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc
      421 tacgtaacac ctctgtactc agcttatctc gggcctccac accgctcgtc acatcctgtg
      481 atccactctt catcccgcta cgtaacacct ctcttaggtg tccctagaat gactatcgat
      541 tttagagctg ccgcctccac accgctcgtc acatcctgtg atccactctt catcccgcta
      601 cgtaacacct ctgtactcag cttatctcgg gcctccacac cgctcgtcac atcctgtgat
      661 ccactcttca tcccgctacg taacacctct cttaggtgtc cctagaattg atatcgattt
      721 tagagctgcc gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg
      781 taacacctct gtactcagct tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc
      841 actcttcatc ccgctacgta acacctctct taggtgtccc tagaattact agagatcgat
      901 tttagagctg ccgcctccac accgctcgtc acatcctgtg atccactctt catcccgcta
      961 cgtaacacct ctgtactcag cttatctcgg gcctccacac cgctcgtcac atcctgtgat
     1021 ccactcttca tcccgctacg taacacctct cttaggtgtc cctagaatga gtatcgattt
     1081 tagagctgcc gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg
     1141 taacacctct gtactcagct tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc
     1201 actcttcatc ccgctacgta acacctctct taggtgtccc tagaatttcc atcgatttta
     1261 gagctgccgc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc ccgctacgta
     1321 acacctctgt actcagctta tctcgggcct ccacaccgct cgtcacatcc tgtgatccac
     1381 tcttcatccc gctacgtaac acctctctta ggtgtcccta gaatgactat cgattttaga
     1441 gctgccgcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc gctacgtaac
     1501 acctctgtac tcagcttatc tcgggcctcc acaccgctcg tcacatcctg tgatccactc
     1561 ttcatcccgc tacgtaacac ctctcttagg tgtccctaga attgatatcg attttagagc
     1621 tgccgcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc tacgtaacac
     1681 ctctgtactc agcttatctc gggcctccac accgctcgtc acatcctgtg atccactctt
     1741 catcccgcta cgtaacacct ctcttaggtg tccctagaat tactagagat cgattttaga
     1801 gctgccgcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc gctacgtaac
     1861 acctctgtac tcagcttatc tcgggcctcc acaccgctcg tcacatcctg tgatccactc
     1921 ttcatcccgc tacgtaacac ctctcttagg tgtccctaga atgagtatcg attttagagc
     1981 tgccgcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc tacgtaacac
     2041 ctctgtactc agcttatctc gggcctccac accgctcgtc acatcctgtg atccactctt
     2101 catcccgcta cgtaacacct ctcttaggtg tccctagaat ttccatcgat tttagagctg
     2161 ccgcctccac accgctcgtc acatcctgtg atccactctt catcccgcta cgtaacacct
     2221 ctgtactcag cttatctcgg gcctccacac cgctcgtcac atcctgtgat ccactcttca
     2281 tcccgctacg taacacctct cttaggtgtc cctagaatga ctatcgattt tagagctgcc
     2341 gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct
     2401 gtactcagct tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc
     2461 ccgctacgta acacctctct taggtgtccc tagaattgat atcgatttta gagctgccgc
     2521 ctccacaccg ctcgtcacat cctgtgatcc actcttcatc ccgctacgta acacctctgt
     2581 actcagctta tctcgggcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc
     2641 gctacgtaac acctctctta ggtgtcccta gaattactag agatcgattt tagagctgcc
     2701 gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct
     2761 gtactcagct tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc
     2821 ccgctacgta acacctctct taggtgtccc tagaatgagt atcgatttta gagctgccgc
     2881 ctccacaccg ctcgtcacat cctgtgatcc actcttcatc ccgctacgta acacctctgt
     2941 actcagctta tctcgggcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc
     3001 gctacgtaac acctctctta ggtgtcccta gaatttccat cgattttaga gctgccgcct
     3061 ccacaccgct cgtcacatcc tgtgatccac tcttcatccc gctacgtaac acctctgtac
     3121 tcagcttatc tcgggcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc
     3181 tacgtaacac ctctcttagg tgtccctaga atgactatcg attttagagc tgccgcctcc
     3241 acaccgctcg tcacatcctg tgatccactc ttcatcccgc tacgtaacac ctctgtactc
     3301 agcttatctc gggcctccac accgctcgtc acatcctgtg atccactctt catcccgcta
     3361 cgtaacacct ctcttaggtg tccctagaat tgatatcgat tttagagctg ccgcctccac
     3421 accgctcgtc acatcctgtg atccactctt catcccgcta cgtaacacct ctgtactcag
     3481 cttatctcgg gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg
     3541 taacacctct cttaggtgtc cctagaatta ctagagccag gcatcaaata aaacgaaagg
     3601 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     3661 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//
