LOCUS       sequence-311346-        7329 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LBS-tetO hybrid sponge with 80 repeats of LBS and 40 repeats of 
            tetO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: PXWS80LBS40tetO
FEATURES             Location/Qualifiers
     source          1..7329
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5379..5397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5426..5444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5475..5493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5507..5525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5549..5567
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5595..5613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5637..5655
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5685..5703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5734..5752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5776..5794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5839..5857
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5886..5904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5935..5953
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5967..5985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6009..6027
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6055..6073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6097..6115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6145..6163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6194..6212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6236..6254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6299..6317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6346..6364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6395..6413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6427..6445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6469..6487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6515..6533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6557..6575
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6605..6623
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6654..6672
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6696..6714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6759..6777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6806..6824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6855..6873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6887..6905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6929..6947
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6975..6993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7017..7035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7065..7083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7114..7132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7156..7174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      7209..7280
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7296..7323
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gctcatactg cgtaagagtc tttaacataa gcacctgtag gatcgtacag gtttacgcta
       61 ggctcctttg tcaaaagtga tcgctaacat aagcacctgt aggatcgtac aggtttacgc
      121 cttcacggga taatgtgttc tacaacataa gcacctgtag gatcgtacag gtttacgcgc
      181 ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct gtaggatcgt acaggtttac
      241 gcaactccgt catcgtggat gtcaatattg gcaacataag cacctgtagg atcgtacagg
      301 tttacgcttg atttcaccgc tggcgtcaat gagtgctcat actgcgtaag agtctttaac
      361 ataagcacct gtaggatcgt acaggtttac gctaggctcc tttgtcaaaa gtgatcgcta
      421 acataagcac ctgtaggatc gtacaggttt acgccttcac gggataatgt gttctacaac
      481 ataagcacct gtaggatcgt acaggtttac gcgcttaagt caatccaatg cgttcaggtg
      541 caacataagc acctgtagga tcgtacaggt ttacgcaact ccgtcatcgt ggatgtcaat
      601 attggcaaca taagcacctg taggatcgta caggtttacg cttgatttca ccgctggcgt
      661 caatttccgc tcatactgcg taagagtctt taacataagc acctgtagga tcgtacaggt
      721 ttacgctagg ctcctttgtc aaaagtgatc gctaacataa gcacctgtag gatcgtacag
      781 gtttacgcct tcacgggata atgtgttcta caacataagc acctgtagga tcgtacaggt
      841 ttacgcgctt aagtcaatcc aatgcgttca ggtgcaacat aagcacctgt aggatcgtac
      901 aggtttacgc aactccgtca tcgtggatgt caatattggc aacataagca cctgtaggat
      961 cgtacaggtt tacgcttgat ttcaccgctg gcgtcaatga ctgctcatac tgcgtaagag
     1021 tctttaacat aagcacctgt aggatcgtac aggtttacgc taggctcctt tgtcaaaagt
     1081 gatcgctaac ataagcacct gtaggatcgt acaggtttac gccttcacgg gataatgtgt
     1141 tctacaacat aagcacctgt aggatcgtac aggtttacgc gcttaagtca atccaatgcg
     1201 ttcaggtgca acataagcac ctgtaggatc gtacaggttt acgcaactcc gtcatcgtgg
     1261 atgtcaatat tggcaacata agcacctgta ggatcgtaca ggtttacgct tgatttcacc
     1321 gctggcgtca attactagag gctcatactg cgtaagagtc tttaacataa gcacctgtag
     1381 gatcgtacag gtttacgcta ggctcctttg tcaaaagtga tcgctaacat aagcacctgt
     1441 aggatcgtac aggtttacgc cttcacggga taatgtgttc tacaacataa gcacctgtag
     1501 gatcgtacag gtttacgcgc ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct
     1561 gtaggatcgt acaggtttac gcaactccgt catcgtggat gtcaatattg gcaacataag
     1621 cacctgtagg atcgtacagg tttacgcttg atttcaccgc tggcgtcaat gagtgctcat
     1681 actgcgtaag agtctttaac ataagcacct gtaggatcgt acaggtttac gctaggctcc
     1741 tttgtcaaaa gtgatcgcta acataagcac ctgtaggatc gtacaggttt acgccttcac
     1801 gggataatgt gttctacaac ataagcacct gtaggatcgt acaggtttac gcgcttaagt
     1861 caatccaatg cgttcaggtg caacataagc acctgtagga tcgtacaggt ttacgcaact
     1921 ccgtcatcgt ggatgtcaat attggcaaca taagcacctg taggatcgta caggtttacg
     1981 cttgatttca ccgctggcgt caatttccgc tcatactgcg taagagtctt taacataagc
     2041 acctgtagga tcgtacaggt ttacgctagg ctcctttgtc aaaagtgatc gctaacataa
     2101 gcacctgtag gatcgtacag gtttacgcct tcacgggata atgtgttcta caacataagc
     2161 acctgtagga tcgtacaggt ttacgcgctt aagtcaatcc aatgcgttca ggtgcaacat
     2221 aagcacctgt aggatcgtac aggtttacgc aactccgtca tcgtggatgt caatattggc
     2281 aacataagca cctgtaggat cgtacaggtt tacgcttgat ttcaccgctg gcgtcaatga
     2341 ctgctcatac tgcgtaagag tctttaacat aagcacctgt aggatcgtac aggtttacgc
     2401 taggctcctt tgtcaaaagt gatcgctaac ataagcacct gtaggatcgt acaggtttac
     2461 gccttcacgg gataatgtgt tctacaacat aagcacctgt aggatcgtac aggtttacgc
     2521 gcttaagtca atccaatgcg ttcaggtgca acataagcac ctgtaggatc gtacaggttt
     2581 acgcaactcc gtcatcgtgg atgtcaatat tggcaacata agcacctgta ggatcgtaca
     2641 ggtttacgct tgatttcacc gctggcgtca attactagag gctcatactg cgtaagagtc
     2701 tttaacataa gcacctgtag gatcgtacag gtttacgcta ggctcctttg tcaaaagtga
     2761 tcgctaacat aagcacctgt aggatcgtac aggtttacgc cttcacggga taatgtgttc
     2821 tacaacataa gcacctgtag gatcgtacag gtttacgcgc ttaagtcaat ccaatgcgtt
     2881 caggtgcaac ataagcacct gtaggatcgt acaggtttac gcaactccgt catcgtggat
     2941 gtcaatattg gcaacataag cacctgtagg atcgtacagg tttacgcttg atttcaccgc
     3001 tggcgtcaat gagtgctcat actgcgtaag agtctttaac ataagcacct gtaggatcgt
     3061 acaggtttac gctaggctcc tttgtcaaaa gtgatcgcta acataagcac ctgtaggatc
     3121 gtacaggttt acgccttcac gggataatgt gttctacaac ataagcacct gtaggatcgt
     3181 acaggtttac gcgcttaagt caatccaatg cgttcaggtg caacataagc acctgtagga
     3241 tcgtacaggt ttacgcaact ccgtcatcgt ggatgtcaat attggcaaca taagcacctg
     3301 taggatcgta caggtttacg cttgatttca ccgctggcgt caatttccgc tcatactgcg
     3361 taagagtctt taacataagc acctgtagga tcgtacaggt ttacgctagg ctcctttgtc
     3421 aaaagtgatc gctaacataa gcacctgtag gatcgtacag gtttacgcct tcacgggata
     3481 atgtgttcta caacataagc acctgtagga tcgtacaggt ttacgcgctt aagtcaatcc
     3541 aatgcgttca ggtgcaacat aagcacctgt aggatcgtac aggtttacgc aactccgtca
     3601 tcgtggatgt caatattggc aacataagca cctgtaggat cgtacaggtt tacgcttgat
     3661 ttcaccgctg gcgtcaatga ctgctcatac tgcgtaagag tctttaacat aagcacctgt
     3721 aggatcgtac aggtttacgc taggctcctt tgtcaaaagt gatcgctaac ataagcacct
     3781 gtaggatcgt acaggtttac gccttcacgg gataatgtgt tctacaacat aagcacctgt
     3841 aggatcgtac aggtttacgc gcttaagtca atccaatgcg ttcaggtgca acataagcac
     3901 ctgtaggatc gtacaggttt acgcaactcc gtcatcgtgg atgtcaatat tggcaacata
     3961 agcacctgta ggatcgtaca ggtttacgct tgatttcacc gctggcgtca attactagag
     4021 gctcatactg cgtaagagtc tttaacataa gcacctgtag gatcgtacag gtttacgcta
     4081 ggctcctttg tcaaaagtga tcgctaacat aagcacctgt aggatcgtac aggtttacgc
     4141 cttcacggga taatgtgttc tacaacataa gcacctgtag gatcgtacag gtttacgcgc
     4201 ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct gtaggatcgt acaggtttac
     4261 gcaactccgt catcgtggat gtcaatattg gcaacataag cacctgtagg atcgtacagg
     4321 tttacgcttg atttcaccgc tggcgtcaat gagtgctcat actgcgtaag agtctttaac
     4381 ataagcacct gtaggatcgt acaggtttac gctaggctcc tttgtcaaaa gtgatcgcta
     4441 acataagcac ctgtaggatc gtacaggttt acgccttcac gggataatgt gttctacaac
     4501 ataagcacct gtaggatcgt acaggtttac gcgcttaagt caatccaatg cgttcaggtg
     4561 caacataagc acctgtagga tcgtacaggt ttacgcaact ccgtcatcgt ggatgtcaat
     4621 attggcaaca taagcacctg taggatcgta caggtttacg cttgatttca ccgctggcgt
     4681 caatttccgc tcatactgcg taagagtctt taacataagc acctgtagga tcgtacaggt
     4741 ttacgctagg ctcctttgtc aaaagtgatc gctaacataa gcacctgtag gatcgtacag
     4801 gtttacgcct tcacgggata atgtgttcta caacataagc acctgtagga tcgtacaggt
     4861 ttacgcgctt aagtcaatcc aatgcgttca ggtgcaacat aagcacctgt aggatcgtac
     4921 aggtttacgc aactccgtca tcgtggatgt caatattggc aacataagca cctgtaggat
     4981 cgtacaggtt tacgcttgat ttcaccgctg gcgtcaatga ctgctcatac tgcgtaagag
     5041 tctttaacat aagcacctgt aggatcgtac aggtttacgc taggctcctt tgtcaaaagt
     5101 gatcgctaac ataagcacct gtaggatcgt acaggtttac gccttcacgg gataatgtgt
     5161 tctacaacat aagcacctgt aggatcgtac aggtttacgc gcttaagtca atccaatgcg
     5221 ttcaggtgca acataagcac ctgtaggatc gtacaggttt acgcaactcc gtcatcgtgg
     5281 atgtcaatat tggcaacata agcacctgta ggatcgtaca ggtttacgct tgatttcacc
     5341 gctggcgtca attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     5401 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     5461 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     5521 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     5581 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     5641 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     5701 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     5761 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     5821 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
     5881 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
     5941 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
     6001 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
     6061 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
     6121 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
     6181 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
     6241 atcagtgata gagacttagg tgtccctaga attactagag atcgatttta gagctgcctc
     6301 cctatcagtg atagagattg ctacaactgc aaggtagtcc cggtatccct atcagtgata
     6361 gagactgata gctacgagtc tgagatccta cttgtcccta tcagtgatag agacagtacc
     6421 gagttttccc tatcagtgat agagagctca tactgcgaca gagtcttttc cctatcagtg
     6481 atagagatag gctcctttgt caaaagtgat cgcttcccta tcagtgatag agacttcacg
     6541 ggataatgtg ttctactccc tatcagtgat agagagctta agtcaatcca atgcgttcag
     6601 gtgctcccta tcagtgatag agaaactccg tcatcgtgga tgtcaatact gactccctat
     6661 cagtgataga gattgatttc accgctggcg tcaattccct atcagtgata gagacttagg
     6721 tgtccctaga attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     6781 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     6841 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     6901 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     6961 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     7021 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     7081 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     7141 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     7201 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     7261 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     7321 gcgtttata
//
