LOCUS       sequence-311348-        3257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LBS-tetO hybrid sponge with 20 repeats of LBS and 20 repeat of 
            Pecf11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: PXWS20LBS20Pecf11
FEATURES             Location/Qualifiers
     source          1..3257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      3137..3208
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3224..3251
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gctcatactg cgtaagagtc tttaacataa gcacctgtag gatcgtacag gtttacgcta
       61 ggctcctttg tcaaaagtga tcgctaacat aagcacctgt aggatcgtac aggtttacgc
      121 cttcacggga taatgtgttc tacaacataa gcacctgtag gatcgtacag gtttacgcgc
      181 ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct gtaggatcgt acaggtttac
      241 gcaactccgt catcgtggat gtcaatattg gcaacataag cacctgtagg atcgtacagg
      301 tttacgcttg atttcaccgc tggcgtcaat gagtgctcat actgcgtaag agtctttaac
      361 ataagcacct gtaggatcgt acaggtttac gctaggctcc tttgtcaaaa gtgatcgcta
      421 acataagcac ctgtaggatc gtacaggttt acgccttcac gggataatgt gttctacaac
      481 ataagcacct gtaggatcgt acaggtttac gcgcttaagt caatccaatg cgttcaggtg
      541 caacataagc acctgtagga tcgtacaggt ttacgcaact ccgtcatcgt ggatgtcaat
      601 attggcaaca taagcacctg taggatcgta caggtttacg cttgatttca ccgctggcgt
      661 caatttccgc tcatactgcg taagagtctt taacataagc acctgtagga tcgtacaggt
      721 ttacgctagg ctcctttgtc aaaagtgatc gctaacataa gcacctgtag gatcgtacag
      781 gtttacgcct tcacgggata atgtgttcta caacataagc acctgtagga tcgtacaggt
      841 ttacgcgctt aagtcaatcc aatgcgttca ggtgcaacat aagcacctgt aggatcgtac
      901 aggtttacgc aactccgtca tcgtggatgt caatattggc aacataagca cctgtaggat
      961 cgtacaggtt tacgcttgat ttcaccgctg gcgtcaatga ctgctcatac tgcgtaagag
     1021 tctttaacat aagcacctgt aggatcgtac aggtttacgc taggctcctt tgtcaaaagt
     1081 gatcgctaac ataagcacct gtaggatcgt acaggtttac gccttcacgg gataatgtgt
     1141 tctacaacat aagcacctgt aggatcgtac aggtttacgc gcttaagtca atccaatgcg
     1201 ttcaggtgca acataagcac ctgtaggatc gtacaggttt acgcaactcc gtcatcgtgg
     1261 atgtcaatat tggcaacata agcacctgta ggatcgtaca ggtttacgct tgatttcacc
     1321 gctggcgtca attactagag atcgatttta gagctgccgc ctccacaccg ctcgtcacat
     1381 cctgtgatcc actcttcatc ccgctacgta acacctctgt actcagctta tctcgggcct
     1441 ccacaccgct cgtcacatcc tgtgatccac tcttcatccc gctacgtaac acctctctta
     1501 ggtgtcccta gaatgagtat cgattttaga gctgccgcct ccacaccgct cgtcacatcc
     1561 tgtgatccac tcttcatccc gctacgtaac acctctgtac tcagcttatc tcgggcctcc
     1621 acaccgctcg tcacatcctg tgatccactc ttcatcccgc tacgtaacac ctctcttagg
     1681 tgtccctaga atttccatcg attttagagc tgccgcctcc acaccgctcg tcacatcctg
     1741 tgatccactc ttcatcccgc tacgtaacac ctctgtactc agcttatctc gggcctccac
     1801 accgctcgtc acatcctgtg atccactctt catcccgcta cgtaacacct ctcttaggtg
     1861 tccctagaat gactatcgat tttagagctg ccgcctccac accgctcgtc acatcctgtg
     1921 atccactctt catcccgcta cgtaacacct ctgtactcag cttatctcgg gcctccacac
     1981 cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct cttaggtgtc
     2041 cctagaattg atatcgattt tagagctgcc gcctccacac cgctcgtcac atcctgtgat
     2101 ccactcttca tcccgctacg taacacctct gtactcagct tatctcgggc ctccacaccg
     2161 ctcgtcacat cctgtgatcc actcttcatc ccgctacgta acacctctct taggtgtccc
     2221 tagaattact agagatcgat tttagagctg ccgcctccac accgctcgtc acatcctgtg
     2281 atccactctt catcccgcta cgtaacacct ctgtactcag cttatctcgg gcctccacac
     2341 cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct cttaggtgtc
     2401 cctagaatga gtatcgattt tagagctgcc gcctccacac cgctcgtcac atcctgtgat
     2461 ccactcttca tcccgctacg taacacctct gtactcagct tatctcgggc ctccacaccg
     2521 ctcgtcacat cctgtgatcc actcttcatc ccgctacgta acacctctct taggtgtccc
     2581 tagaatttcc atcgatttta gagctgccgc ctccacaccg ctcgtcacat cctgtgatcc
     2641 actcttcatc ccgctacgta acacctctgt actcagctta tctcgggcct ccacaccgct
     2701 cgtcacatcc tgtgatccac tcttcatccc gctacgtaac acctctctta ggtgtcccta
     2761 gaatgactat cgattttaga gctgccgcct ccacaccgct cgtcacatcc tgtgatccac
     2821 tcttcatccc gctacgtaac acctctgtac tcagcttatc tcgggcctcc acaccgctcg
     2881 tcacatcctg tgatccactc ttcatcccgc tacgtaacac ctctcttagg tgtccctaga
     2941 attgatatcg attttagagc tgccgcctcc acaccgctcg tcacatcctg tgatccactc
     3001 ttcatcccgc tacgtaacac ctctgtactc agcttatctc gggcctccac accgctcgtc
     3061 acatcctgtg atccactctt catcccgcta cgtaacacct ctcttaggtg tccctagaat
     3121 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3181 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     3241 gcctttctgc gtttata
//
