LOCUS       sequence-311352-        3349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLX series: pBBR1-based T-DNA binary vector for plant transformation
            (omega2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 
            10.1021/acssynbio.6b00354. Epub 2017 Jun 28.
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00354"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1962-1968"
COMMENT     Sequence Label: pLX-B3omega2 (GB1695)
FEATURES             Location/Qualifiers
     source          1..3349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        complement(join(531..537,538..555,556..561))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(815..1606)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1828..2490)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2491..3262
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa acggtctcag tcaggagaga gacgaaaagc aaaaacccgc cgaagcgggt
      301 tactagcgcc attcgccatt cagagagcgg agctgctgcg acggacgatc ggtacgcgcc
      361 tcttcgctat tacgccaact ggcgaaaggt ggatgtgctg caaggcgatt aagttgggta
      421 acgccagggt tttcccagtc acgacgttgt agtaccacgg caaggctatc tgtaatcatt
      481 gttgtactcc ggttaggacg gattgggaac tggctaactc aaaatccaca cattatacga
      541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgacgtctct cgctggagac
      601 cggacaagga tgcgagctag cctgcaggaa ttgttgattt tgtgatgact gatggcagga
      661 tatatgcggt tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgttcggt
      721 tgtaaatttt tttggaagaa caagaaaaga aaaaacaccc gttagggtgt ttttagttag
      781 tgtggcgcgc cgacttgcga catgcggtcc tttgttattt gccgactacc ttggtgatct
      841 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
      901 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
      961 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
     1021 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
     1081 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
     1141 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
     1201 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
     1261 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
     1321 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
     1381 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     1441 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
     1501 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     1561 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg
     1621 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     1681 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
     1741 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     1801 ttcgatcgga ttggcggtta tgcggttcta ccggcgcggc agcgttaccc gtgtcggcgg
     1861 ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg
     1921 cccgcgccgt tcccattcct ccgtttcggt caaggctggc aggtctggtt ccatgcccgg
     1981 aatgccgggc tggctgggcg gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg
     2041 atacagggtc gggatgcggc gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc
     2101 gtgatcaacc accacggcgg cactgaacac cgacaggcgc aactggtcgc ggggctggcc
     2161 ccacgccacg cggtcattga ccacgtaggc cgacacggtg ccggggccgt tgagcttcac
     2221 gacggagatc cagcgctcgg ccaccaagtc cttgactgcg tattggaccg tccgcaaaga
     2281 acgtccgatg agcttggaaa gtgtcttctg gctgaccacc acggcgttct ggtggcccat
     2341 ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc
     2401 ccacgcctca tgcgctttgc gttccgtttg cacccagtga ccgggcttgt tcttggcttg
     2461 aatgccgatt tctctggact gcgtggccat gcttatctcc atgcggtagg ggtgccgcac
     2521 ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca gcgcgacaac aattatgcgt
     2581 tgcgtaaaag tggcagtcaa ttacagattt tctttaacct acgcaatgag ctattgcggg
     2641 gggtgccgca atgagctgtt gcgtaccccc cttttttaag ttgttgattt ttaagtcttt
     2701 cgcatttcgc cctatatcta gttctttggt gcccaaagaa gggcacccct gcggggttcc
     2761 cccacgcctt cggcgcggct ccccctccgg caaaaagtgg cccctccggg gcttgttgat
     2821 cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc cccttggaac ccccgcactc
     2881 gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc cttcgactgc ccccactcgc
     2941 ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc
     3001 cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg
     3061 aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt
     3121 ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc
     3181 tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg
     3241 aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg
     3301 agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtaggggg
//
