LOCUS       sequence-31136-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Tet-On vector for CMV-driven rtTA with EGFP and 
            Blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2640
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(111..728,729..734,735..770,771..773,774..809,810..812,
                     813..848,849..854)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 agcagagctc gtttagtgac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcacc atgtctagac
      121 tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc ggtatcgaag
      181 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
      241 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
      301 atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
      361 agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
      421 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
      481 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
      541 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
      601 attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac
      661 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
      721 aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc ccagccgatg
      781 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
      841 acatgctccc cggntacnnn annaaggatc ccccgggctg cagaattcga tgnccgcccc
      901 ctctccctcc ccccccccta acgttactgg gcnaagccnc ttggaataag
//
