LOCUS       sequence-31137-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2641
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(78..188)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 agcggcttcg gccagtaacg ttaggggggg ggggagggag agggggcggc cgggggatcc
       61 ttacttagtt acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc
      121 atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca
      181 agggcgtcgg ccggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt
      241 agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct
      301 tgtctcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact
      361 tgatgctcct gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg
      421 tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg
      481 gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt
      541 gagaggagag cacagcggaa tgacttggcg ttgttccgca gaaagtcttg ccatgactcg
      601 ccttccaggg ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg
      661 agcagggccc gcttgntctt cacgtgccag tacagggtag gctgctcaac tcccagcttt
      721 tgagcgagtt tnccttgtcg tcaggncttc gataccgact ccattgagta nnncagagcg
      781 ncntnatgac tttgctctnt ccagtctaga catgtgaatc ctgcagcccg c
//
