LOCUS       sequence-31138-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2641
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..573
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(183..202)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     412..429
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(630..740)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtattatc gtgtttttca aagggaaacc acgtccccgt
       61 ggttcggggg gcctagacgt ttttttaacc tcgactaaac acatgtaaag catgtgcacc
      121 gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt
      181 tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc
      241 actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc
      301 acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc
      361 ttcaagaagc ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt
      421 tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg
      481 ccctcacatt gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc
      541 ggccttattc caagcggctt cggccagtaa cgttaggggg ggggggangg aganggggcg
      601 gccgggggat ccttacttag ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg
      661 tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag
      721 tcaaaatcgt caagggcgtc ggccggcccg ccgctttcgc actttagctg tttctccagg
      781 ccacatatga ttagttccag gccgaaaagg nangcaggtt cggntccctg ccggtcgaac
      841 agctcaattg cttgtctcag aagtgggggc atagaatcgg
//
