LOCUS       sequence-31139-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2706
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..127
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(176..192)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..192)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(201..758)
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFAXICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     regulatory      755..764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(803..827)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(824..862)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    874..892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    910..928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    945..963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc
       61 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac
      121 acattccaca gggtcctcca ccgcggtggc ggccatcaat tcctgcagcc cgggggatcc
      181 actagttcta gagcggccgc ttagtcacca ccggccccct tgatcttgtc cacctggccc
      241 tggatcttgc tggcaaaggt cgcacagcgt tgcggcagcc acttcttgag caggtcagaa
      301 cactgcacgt tggcaagccc tttgaggcag ccagttgtgc agtccacaca cagatcgacc
      361 tgtgcgatga actgctccat gggctccaag tccttgaacc caggaatctc aggaatgtcg
      421 acgatcgcct cgcctatgcc gccctgtgcg gactctttgt cgccttcgta ggtgtggcag
      481 cgtcctggga tgaacttctt catcttgggc gtgcacttga tgtgggacag gcagatcaga
      541 cagcccctgg tgcagccagc tttccgggca ttggcttcca tctctttgag cacctccagc
      601 ggcagcttct tgccgggcaa cttcccgcgg tcagcatcga gatccgtggt cgcgaagttg
      661 ctggccacgg ccacgatgtt gaagtcttcg ttgttctcgg tgggcttggc ctcggccaca
      721 gcgatgcaga tcanggcaaa cagaactttg actcccatgg tggctggatc cgagctcggt
      781 accgagctcg aattctccan gcgatctgac ggttcactaa acgagctctg cttatatagg
      841 cctcccaccg tacacgccta cctcgacata cnttctctat cactgatagg gagtaaactc
      901 gacatacntt ctctatcact gatagggata aactcgacat acgttctcta tcactgatag
      961 ggagtaaact cgacatac
//
