LOCUS       sequence-31141-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2780
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..116
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(165..181)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(165..181)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     239..255
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(280..594)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(283..307)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    354..372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    390..408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    425..443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    461..479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    497..515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    532..550
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    568..586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     598..614
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
       61 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
      121 ggtcctccac cgcggtggcg gccatcaatt cctgcagccc gggggatcca ctagttctag
      181 agcggccgcc accgcggtgg agctcgaatt gacgcgtcct cggtaccggg ncccccctcg
      241 aggtcgacgg tatcgataag cttgatatcg aattctccag gcgatctgac ggttcactaa
      301 acgagctctg cttatatagg cctcccaccg tacacgccta cctcgacata cgttctctat
      361 cactgatagg gagtaaactc gacatacgtt ctctatcact gatagggata aactcgacat
      421 acgttctcta tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag
      481 taaactcgac atacgttctc tatcactgat agggagtaaa ctcgacatcg ttctctatca
      541 ctgataggga gtaaactcga catacgttct ctatcactga tagggagtaa actcgatcga
      601 ggtcgacggt atcgataagc ttgatggggg atctggcggt aatacgtcta gctagagcgg
      661 gccggcccgg tactaaccaa actggatctc tgctgtccct gtaataaacc cgaaaatttt
      721 gaatttttg
//
