LOCUS       sequence-31142-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2780
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(196..300)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(379..398)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        574..960
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attggaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
       61 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      301 cacatttccc cgaaaagtgc cacctgacgt cgatcgacgg atcgggagat ctcccgatcc
      361 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      421 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      481 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      541 tcgcgatgta cgggccagat atacgcgcgc gttgacattg attattgact agctagttat
      601 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      661 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      721 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatggggt
      781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      841 gccccctatt gacgtcaatg acggtaaaat ggcccgcctg gcattatgcc cagtacatga
      901 ccttatgggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      961 g
//
