LOCUS       sequence-31145-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2867
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           320..346
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     protein_bind    473..494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(509..514,515..532,533..539)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    547..563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     552..574
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             583..756
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(603..620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(603..619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(612..634)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     ncRNA           776..817
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(872..892)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cagcgaagcg gatgtacccc gagganggcg ccctgaaggg cgagatcaag cagcggctga
       61 agctgaagga cggcggccac tacgacgccg aggtgaagac cacctacaag gccaagaagc
      121 ccgtgcagct ccccggcgcc tacaacgtga acatcaagct ggacatcacc agccacaacg
      181 aggactacac catcgtggag cagtacgagc gggccgaggg ccggcacagc accggcggca
      241 tggacgagct gtacaagtaa gctaagcact tcgtggccgt cgatcgttta aagggaggta
      301 gtgagtcgac cagtggatcc tggaggcttg ctgaaggctg tatgctgatc gcaggtcgcg
      361 ggacgtcgcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      421 gctggcacgc caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      481 gttagctcac tcattaggca ccccaggctt tacaccttat gcttccggct cgtatgttgt
      541 gtggaattgt gagcggataa caatttcaca caggaaacgg ctatgaccat gattacggat
      601 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      661 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      721 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcacctgcga tccaggacac
      781 aaggcctgtt actagcactc acatggaaca aatggcccag atcccccggg ctgcaggaat
      841 tgatggccgc caccgcggtg gaggaccctg tggaatgtgt gtcagttagg gtgtggaaag
      901 tccccaggnt ccccagcagg cagaagtatg caaagcatgc atctcaat
//
