LOCUS       sequence-311457-       10691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, empty.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S
FEATURES             Location/Qualifiers
     source          1..10691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1746..1760,1761..1766,1767..1783,1784..1790)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1766..1782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1798..1814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1833..1844
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1851..1877,1878..1880,1881..4367,4368..4370,
                     4371..5957)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6127..6918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7041..7629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7530..7549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agttctttta aaagagaatt tagtaaagcc ctaagtcttt taataaaaaa ttttttgtaa
       61 aaaagttggc aaaaatagtt tttgctatat atttatatat aagaaaaaat tcaatactca
      121 aggagataaa aaatggaaaa cttaaacaat aatccacaaa aaattaatca aataaataag
      181 caaactaatt ttattggttt tgaaaatttt atagaacttg ataatcaagt taatcaaact
      241 aaagaaaaaa gtaaattaag taagataggt aagaaattac ccggaataag cagtcaagtc
      301 tagtgcggcc ggcgcgccaa ataatgcccg tgtccctgtt gaatagggac acggtttttt
      361 aacaattttt ttattcctag gccttagtgg ccggtacccg gggatcctta ttactggccg
      421 ctttctagcc tggatacttg tctggctagt tgcattagag agtcagctag agccctaggg
      481 ctagctgttt gtgtattcgg agccagtgta gtttttcttt ttactaacga tacgggtaat
      541 aattgattgc cttttacagc ctgaccttga gacaattgca aaagtctgtc cacagaggtt
      601 tgacctaata atctaaaatc ttgtttgata gtagtaagtg gaggtatgta gcaactagaa
      661 tcttctgtat catcataacc cacaactgaa atatcagccc caactctaag tccactttca
      721 gtaatagctc gcatagctcc taaagccatt tgatcattag caactaacat agcagtaggg
      781 acaatgcctt catttaacat ttgcattgtt tgctgaaatc ctgacatagc agaccaatct
      841 ccttctcttt ctgctattgg ttgtatttga tttcttgtta aatacttgtg ccatcctgct
      901 aatctcaatc tggcacttac tgaacttaag ggacctgcaa gtagtgcaat ttgttgatgg
      961 cctaatgcaa caaggtgttc aactcctaac cttgttccat cttcgtgaga aaaaattata
     1021 gagtttattg gggtttggtc cgacacatcc aaaaacaatg ctggaacatt agtacatgct
     1081 gcttcaactg caatcgcatc ttggtcatca agaggataat taattataag tccactaaca
     1141 cgttgagcta ataaattatg tactgctgcc ttacaagcct ctacacctga tctctcaacc
     1201 attgatacaa caacgcttgc accaagttga tcagctctcg atttaattgc ggctacaatt
     1261 tgactaggcg catgtaatgc aagtgatgat gtggcaactc caattaaaag tgattgtttc
     1321 cccgcaagtt gctgagcaac gcgatttggt atataattca actctgccat agctgcttca
     1381 accttctctc ttgtttttgc agatacatga cttgcttggt tcacaactct cgaaaccgtt
     1441 tggtagctaa ccccggcata ttcagcaaca tcatacaaag taacaggttt catggatcat
     1501 tcctccatga taaaatttaa atttctgact ttggcaaatc cttttgcctg ttaaaaagaa
     1561 tagcacaaaa attaaaaaaa aagaaacgca aatagcaata gcattaaaaa taattattat
     1621 tatttatttc tttgattaaa atcaaaagcc tttaatgtat tttcttgatc tttcatgcgc
     1681 ttaacggtaa aatccaaggt attaagttca ccttccactt ttctctctct atcttctcta
     1741 gaaaatcata aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg
     1801 tgagcggata acaatttcac acagaattca ttaaagagga gaaattacat atggataaga
     1861 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
     1921 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
     1981 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
     2041 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
     2101 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
     2161 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
     2221 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
     2281 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
     2341 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
     2401 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
     2461 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
     2521 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
     2581 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
     2641 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
     2701 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
     2761 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
     2821 gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc
     2881 cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg
     2941 atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg
     3001 atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga
     3061 cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga
     3121 gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct
     3181 tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat
     3241 ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata
     3301 aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa
     3361 atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat
     3421 tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac
     3481 agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat
     3541 taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg
     3601 aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata
     3661 aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga
     3721 ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg
     3781 atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc
     3841 gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta gattttttga
     3901 aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat
     3961 ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata
     4021 ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg
     4081 ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg
     4141 cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa
     4201 tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata
     4261 ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg
     4321 tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc attgttccac
     4381 aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc
     4441 gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga
     4501 gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg
     4561 aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa
     4621 ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg
     4681 atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt
     4741 ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg
     4801 cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac
     4861 ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta
     4921 agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga
     4981 acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg
     5041 aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc
     5101 gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagtc cagacaggcg
     5161 gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa
     5221 aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc
     5281 tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac
     5341 tagggatcac aattctggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag
     5401 ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt
     5461 ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa
     5521 atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa
     5581 agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc
     5641 attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag
     5701 atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg
     5761 aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt
     5821 ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag
     5881 atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc
     5941 agctaggagg tgactaataa aagcttgatc agatctcagc ttttttttga agtgcctggc
     6001 agtaagttga aaaaatttga aataaacttg gactatgtta ataaaaaaat acaaattaac
     6061 gaacaaaaag atttgaatta ttatataatt tagttaaaag caattttaaa tgagggaggt
     6121 ttccatatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     6181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     6241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     6301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     6361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     6421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     6481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     6541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     6601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     6661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     6721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     6781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     6841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     6901 aaggtagtcg gcaaataatg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     6961 gttctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7681 gctcacatgt tctttcctgc actagatccc cggcaagttc atccttaggg ttttcatatc
     7741 tcttaaaaag tcaatttctg cttgattaat ttcttgtgga ttattgtttt tgcggttttt
     7801 cattttttat ctccttgagt attgaatttt ttcttatata taaatatata gcaaaaacta
     7861 tttttgccaa cttttttaca aaaaattttt tattaaaaca cttagggctt tgctaaattc
     7921 tcttttaaaa gaacttagta aagccctatg gatttaagaa ctgattatac ttacgtagta
     7981 aaaatactac agatattaat agtatagacc atatcaaaat cataatcaat ttatatttat
     8041 ctaaaattgt agtccataca aaatacaaaa aaattaggtt tttctaaatt ctattttaaa
     8101 agaacttagt aacacctaat ctctaaatta cttacaaaat tctaaaatta gatttttagt
     8161 tcttcatatt tctttaaaag ttttttaaga aaatcttttt gattttcaaa aatttcatgc
     8221 atcataaaac tagtaaattt ggattttttt ttgtaaaaat cataactttc ttgggtttta
     8281 agttgaaatc ttaatggttt tattggattt tgcctagatt tttttaacgc cggactttct
     8341 ttatctttta taaaattata agaattttta ataccatttt ctattaaata atcttctttt
     8401 ataagcccct cttgcagtgc agttgctaat cttaaatact tatacgcttg agtttttgct
     8461 acatcaaagt cttttataaa atcaagaaaa gttttataac catcaaattt ataatattga
     8521 ttatctttta tttcttttaa aattttaata ttttccaccc tgtggaatat ttcttttttg
     8581 aaattgtcat ttaacttttc ctttagtgtc ttgtaataag aaattttact ttcattaatc
     8641 attaaatttt gttcaacata agtatctatc ttttttgtta aaactttaac aacacgtttt
     8701 ttttccataa aaattttcct ttatttttgc aacattttcc ttaattgata taattgttac
     8761 tattttagat tttttaatat ccacttaaga attaatccct cactagaata ttttttatta
     8821 tagaattcat atacatgttt ttctaaatct ataaatttat caacaattgc tttattatag
     8881 ggtgtatcta tcttttcttt tcttaataat cgtctcatat tctttaaata acaacaaaca
     8941 ctgccggttt taaacctaaa ttctatgtaa tatatttttg aaaaagtata tacattttca
     9001 accccgttta tttcatactt tacaaaaaca ttatttattg gttttctata accatagtaa
     9061 atgcctaaaa atttgtcccc gtctttcata ggatatagat aaagtttttg aatctctgat
     9121 ttatctaata atttttcaaa aaaaattata caccgctttt gtttgttact aactactcta
     9181 tgcaatgcct ttaatatttt tgtgtgatat atatttttat cacctttttt ttctttcagc
     9241 aggaagatac tttcttttgg aaatttaatt ttttgttcat ttattcttgc agccttaagt
     9301 ccgattttca tatttaatcc ttatatagcc tttctattta attcttttaa actcaataaa
     9361 cttttttggt ttttcattag ttctaataat tcgcaataat atgtgcaaaa cactttattg
     9421 tattctattt tcttttggtt attcaaatat tcttttataa ttggctttag aagttcaata
     9481 tttacttctt tttttagttg ttcaataagg atattgaaaa tgtttgtttt taaatcttga
     9541 taattttgtt gagaattttc tttttttctt tcaattgact tctctaattt acgctttata
     9601 ttgtttaaat cgctatattt atgactttca ataataaaat gaggcttaaa tttgtaattt
     9661 tcgtatattt tttggaaact tgtttctaat tgtttagaat tatattcatt tttttcaaat
     9721 tctttttgag tgttgcatag aatttccttt aatttttctt gtttttcttt catacaagat
     9781 ttagtaaaat gtatattttt attttttagt aatttaattt ctattatttt tatgatttta
     9841 agtattttga ttatattatc tttatcaata tttaaattga ataaaattgt aagaaaaatt
     9901 gaaagaattt ttttacataa aaagttacat ttattgaagt aatttcttac ttgatacttt
     9961 tctatttcat taatctttct ttcttctttt atattatttt tattatttaa acactccttt
    10021 aaatctacac tcccattttt agtaaatcta tctttaaggc cccttgaaac tctattttga
    10081 aatcttaaat cttttttttc ttgaaagtat ttatagattt tttggtaaca ttcttttttt
    10141 gaaaaattaa gttgatagta tatttcggtc cccaaattta cccctaagtg tttgtgataa
    10201 ttagttgtga ctttaaattc tttttctaat ttgtaaaggt aattttgcag tgtttttagt
    10261 ttaacgggtg tttgactatt tcttttaagg ttttcattaa agtaatagag tatgtttttt
    10321 tgtgtgtatt tcttatatgt tttatttatg tacgctagtg ttgagataag gactactaat
    10381 ttatactgta atttattgta gcaagttgtg gttttactgg ttttgtttat attagataag
    10441 ttttccattt atagctcctt gaataccgga ttttttatct ataaagtata tatatcacaa
    10501 ttttgattta aaagtaaatt ctttttttaa aaatacaaaa attttttata aattatatag
    10561 accatgatat tgtattaata ctaattgatt ttaatttaag gttgaactaa aataaacata
    10621 gttttgtaga aaataatttt tcattatttt gctacttgat attggatcgt aaaaatatta
    10681 gggctttaca a
//
