LOCUS       sequence-31146-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer 
            HE, Keenan RJ, Glick BS
  TITLE     A noncytotoxic DsRed variant for whole-cell labeling.
  JOURNAL   Nat Methods. 2008 Nov . 5(11):955-7.
  PUBMED    18953349
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2008 Nov . 5(11):955-7."
COMMENT     Sequence Label: YIPlac204TC-NLS-DsRed-Express2
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..680)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(65..88)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(263..282)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     480..500
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     CDS             complement(693..713)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccctactgga acaggtggtg gcgggcctcg gcgcgctcgt actgctccac cacggtgtag
       61 tcctcgttgt gggaggtgat gtccagcttg gagtccacgt agtagtagcc gggcagcttc
      121 acgggcttct tggccatgta gattgacttg aactccacca ggtagtggcc gccgcccttc
      181 agcttcagcg ccttgtggat ctcgcccttc agcacgccgt cgcgggggta caggcgctcg
      241 gtggagggct cccagcccag agtcttcttc tgcattacgg ggccgtcgga ggggaagttc
      301 acgccgatga acttcacgtg gtagatgaag gtgccgtcct gcagggagga gtcctgggtc
      361 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
      421 aaggacagct tcttgtagtc ggggatgtcg gcggggtgct tcacgtacac cttggagccg
      481 tactggaact ggggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc
      541 tgcagcttgg cggtctgggt gccctcgtag ggcttgccct cgccctcgcc ctcgatctcg
      601 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa gggcttgatg
      661 acgttctcag tgctatccat ttttgaattg ccgacctttc ttttcttttt tggcattttt
      721 tgaattcttt tagtttatgt atgtgttttt tgtagttata gatttaagca agaaaagaat
      781 acaaacaaaa aattgaaaaa gattgattta gaattaaaaa gaaaaatatt tacgtaagaa
      841 gggaaaatag taaatgttgc aagttcacta aantctaata
//
