LOCUS       sequence-31147-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer 
            HE, Keenan RJ, Glick BS
  TITLE     A noncytotoxic DsRed variant for whole-cell labeling.
  JOURNAL   Nat Methods. 2008 Nov . 5(11):955-7.
  PUBMED    18953349
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2008 Nov . 5(11):955-7."
COMMENT     Sequence Label: YIPlac204TC-NLS-DsRed-Express2
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(165..185)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(200..630)
                     /gene="S. cerevisiae TPI1"
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose 
                     phosphate isomerase"
     primer_bind     complement(754..773)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     873..895
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cccttggtca cctgcagctt ggcggtctgg gtgccctcgt agggcttgcc ctcgccctcg
       61 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
      121 aagggcttga tgacgttctc agtgctatcc atttttgaat tgccgacctt tcttttcttt
      181 tttggcattt tttgaattct tttagtttat gtatgtgttt tttgtagtta tagatttaag
      241 caagaaaaga atacaaacaa aaaattgaaa aagattgatt tagaattaaa aagaaaaata
      301 tttacgtaag aagggaaaat agtaaatgtt gcaagttcac taaactccta aattatgctg
      361 ccctttatat tccctgttac agcagccgag ccaaaggtat ataggctcct ttgcattagc
      421 atgcgtaaca aaccacctgt cagtttcaac cgaggtggta tccgagagaa ttgtgtgatt
      481 gctttaatta atttcggaga atctcacatg ccactgaaga ttaaaaactg gatgccagaa
      541 aaggggtgtc caggtgtaac atcaatagag gaagctgaaa agtcttagaa cgggtaatct
      601 tccaccaacc tgatgggttc ctagatataa tctcgaaggg aataagtagg gtgataccgc
      661 agaagtgtct gaatgtatta aggtccgcct gatgcggtat tttctcctta cgcatctgtg
      721 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      781 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      841 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      901 accgtc
//
