LOCUS       sequence-31148-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     Glick Lab Plasmids
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHS12-mCherry
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(135..200)
                     /codon_start=1
                     /product="mitochondrial presequence of yeast cytochrome c 
                     oxidase subunit IV (Hurt et al., 1984)"
                     /label=pre-CoxIV
                     /translation="MLSLRQSIRFFKPATRTLCSSR"
ORIGIN
        1 gcctcgccct cgccctcgat ctcgaactcg tggccgtcca cggagccctc catgtgcacc
       61 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatg
      121 gtggcgacta gtggtctaga gctacacaaa gttcttgtgg ctggcttgaa aaatcttata
      181 gattgacgta gtgaaagcat tgtgcttgtt atctatttgt atggaattcc ttgattgtat
      241 gcttggtata gcttgaaata ttgtgcagaa aaagaaacaa ggaagaaagg gaacgagaac
      301 aatgacgagg aaacaaaaga ttaataattg caggtctatt tatacttgat agcaagacag
      361 caaacttttt tttatttcaa attcaagtaa ctggaaggaa ggccgtatac cgttgctcat
      421 tagagagtag tgtgcgtgaa tgaaggaagg aaaaagtttc gtgtgcttcg agatacccct
      481 catcagctct ggaacaacga catctgttgg tgctgtcttt gtcgttaatt ttttccttta
      541 gtgtcttcca tcattttttt gtcattgcgg atatggtgag acaacaacgg gggagagaga
      601 aaagaaaaaa aaagaaaaga agttgcatgc gcctattatt acttcaatag atggcaaatg
      661 gaaaaagggt agtgaaactt cgatatgatg atggctatca agtctagggc tacagtatta
      721 gttcgttatg taccaccatc aatgaggcag tgtaattggt gtagtcttgt ttagcccatt
      781 atgtcttgtc tggtatctgt tctattgtat atctcccctc cgccacctac atgttaggga
      841 gaccaacgaa
//
