LOCUS       sequence-311495-        6544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Psyn-mCherry promoter strength reporter for use in B. burgdorferi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBSV2_Psyn-mCherryBb
FEATURES             Location/Qualifiers
     source          1..6544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(746..763)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(746..762)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(755..777)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1329..2138
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1396..1415)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2307..2895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2796..2815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6372..6393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6408..6413,6414..6431,6432..6438)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6446..6462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6451..6473
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6470..6486
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6470..6486
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        join(6522..6544,1..12)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
ORIGIN
        1 tataatacta gtggatccag gaggttcatg gttagtaaag gtgaagaaga taatatggct
       61 attattaaag aatttatgag atttaaagtt cacatggaag gtagtgttaa tggtcatgaa
      121 tttgaaattg aaggtgaagg tgaaggtaga ccttatgaag gtactcaaac tgctaaatta
      181 aaagttacta aaggtggtcc tttacctttt gcttgggata ttttaagtcc tcaatttatg
      241 tatggtagta aagcatacgt taaacatcct gctgatattc ctgattattt aaaattaagt
      301 tttcctgaag gttttaaatg ggaaagagtt atgaattttg aagatggtgg tgttgttact
      361 gttactcaag atagtagttt acaagatggt gaatttattt ataaagttaa attaagaggt
      421 actaattttc ctagtgatgg tcctgttatg caaaaaaaaa ctatgggttg ggaagctagt
      481 agtgaaagaa tgtatcctga agatggtgct ttaaaaggtg aaattaaaca aagattaaaa
      541 ttaaaagatg gtggtcatta tgatgctgaa gttaaaacta cttataaagc taaaaaacct
      601 gttcaattac ctggtgctta taatgttaat attaaattag atattacttc gcataatgaa
      661 gattatacta ttgttgaaca atatgaaaga gctgaaggta gacatagtac aggtggtatg
      721 gatgaattat ataaataagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      781 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      841 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      901 ggcgcggccg ccctaggtta atacccgagc ttcaaggaag atttcctatt aaggttgaac
      961 ttaagagctt aagcatagac gatttgaaaa aaattttaaa acaaacaaaa aattctttaa
     1021 taaaacaata tgttgcgatg tttaaggttt atgatttaga tttaaagttt agtgaggaag
     1081 ctatagatag aattgcagag cttactttta atatgaatct tgagagtgaa aatcttggtg
     1141 ccagaagact tcacggtgtt atggaaatag tgcttgcaga tctttttttt gaagtgcctg
     1201 gcagtaagtt gaaaaaattt gaaataaact tggactatgt taataaaaaa atacaaatta
     1261 acgaacaaaa agatttgaat tattatataa tttagttaaa agcaatttta aatgagggag
     1321 gtttccatat gggccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1381 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1441 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1501 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1561 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     1621 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     1681 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     1741 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     1801 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     1861 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     1921 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     1981 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2041 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2101 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agactcgagt ggccgaggag
     2161 caggactgac acgtgctaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2401 actctttttt cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2941 ccttttgctc acatgttctt tcctgcacta gatccccggc aagttcatcc ttagggtttt
     3001 catatctctt aaaaagtcaa tttctgcttg attaatttct tgtggattat tgtttttgcg
     3061 gtttttcatt ttttatctcc ttgagtattg aattttttct tatatataaa tatatagcaa
     3121 aaactatttt tgccaacttt tttacaaaaa attttttatt aaaacactta gggctttgct
     3181 aaattctctt ttaaaagaac ttagtaaagc cctatggatt taagaactga ttatacttac
     3241 gtagtaaaat actacagata ttaatagtat agaccatatc aaaatcataa tcaatttata
     3301 tttatctaaa attgtagtcc atacaaaata caaaaaaatt aggtttttct aaattctatt
     3361 ttaaaagaac ttagtaacac ctaatctcta aattacttac aaaattctaa aattagattt
     3421 ttagttcttc atatttcttt aaaagttttt taagaaaatc tttttgattt tcaaaaattt
     3481 catgcatcat aaaactagta aatttggatt tttttttgta aaaatcataa ctttcttggg
     3541 ttttaagttg aaatcttaat ggttttattg gattttgcct agattttttt aacgccggac
     3601 tttctttatc ttttataaaa ttataagaat ttttaatacc attttctatt aaataatctt
     3661 cttttataag cccctcttgc agtgcagttg ctaatcttaa atacttatac gcttgagttt
     3721 ttgctacatc aaagtctttt ataaaatcaa gaaaagtttt ataaccatca aatttataat
     3781 attgattatc ttttatttct tttaaaattt taatattttc caccctgtgg aatatttctt
     3841 ttttgaaatt gtcatttaac ttttccttta gtgtcttgta ataagaaatt ttactttcat
     3901 taatcattaa attttgttca acataagtat ctatcttttt tgttaaaact ttaacaacac
     3961 gttttttttc cataaaaatt ttcctttatt tttgcaacat tttccttaat tgatataatt
     4021 gttactattt tagatttttt aatatccact taagaattaa tccctcacta gaatattttt
     4081 tattatagaa ttcatataca tgtttttcta aatctataaa tttatcaaca attgctttat
     4141 tatagggtgt atctatcttt tcttttctta ataatcgtct cgtattcttt aaataacaac
     4201 aaacactgcc ggttttaaac ctaaattcta tgtaatatat ttttgaaaaa gtatatacat
     4261 tttcaacccc gtttatttca tactttacaa aaacattatt tattggtttt ctataaccat
     4321 agtaaatgcc taaaaatttg tccccgtctt tcataggata tagataaagt ttttgaatct
     4381 ctgatttatc taataatttt tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta
     4441 ctctatgcaa tgcctttaat atttttgtgt gatatatatt tttatcacct tttttttctt
     4501 tcagcaggaa gatactttct tttggaaatt taattttttg ttcatttatt cttgcagcct
     4561 taagtccgat tttcatattt aatccttata tagcctttct atttaattct tttaaactca
     4621 ataaactttt ttggtttttc attagttcta ataattcgca ataatatgtg caaaacactt
     4681 tattgtattc tattttcttt tggttattca aatattcttt tataattggc tttagaagtt
     4741 caatatttac ttcttttttt agttgttcaa taaggatatt gaaaatgttt gtttttaaat
     4801 cttgataatt ttgttgagaa ttttcttttt ttctttcaat tgacttctct aatttacgct
     4861 ttatattgtt taaatcgcta tatttatgac tttcaataat aaaatgaggc ttaaatttgt
     4921 aattttcgta tattttttgg aaacttgttt ctaattgttt agaattatat tcattttttt
     4981 caaattcttt ttgagtgttg catagaattt cctttaattt ttcttgtttt tctttcatac
     5041 aagatttagt aaaatgtata tttttatttt ttagtaattt aatttctatt atttttatga
     5101 ttttaagtat tttgattata ttatctttat caatatttaa attgaataaa attgtaagaa
     5161 aaattgaaag aattttttta cataaaaagt tacatttatt gaagtaattt cttacttgat
     5221 acttttctat ttcattaatc tttctttctt cttttatatt atttttatta tttaaacact
     5281 cctttaaatc tacactccca tttttagtaa atctatcttt aaggcccctt gaaactctat
     5341 tttgaaatct taaatctttt ttttcttgaa agtatttata gattttttgg taacattctt
     5401 tttttgaaaa attaagttga tagtatattt cggtccccaa atttacccct aagtgtttgt
     5461 gataattagt tgtgacttta aattcttttt ctaatttgta aaggtaattt tgcagtgttt
     5521 ttagtttaac gggtgtttga ctatttcttt taaggttttc attaaagtaa tagagtatgt
     5581 ttttttgtgt gtatttctta tatgttttat ttatgtacgc tagtgttgag ataaggacta
     5641 ctaatttata ctgtaattta ttgtagcaag ttgtggtttt actggttttg tttatattag
     5701 ataagttttc catttatagc tccttgaata ccggattttt tatctataaa gtatatatat
     5761 cacaattttg atttaaaagt aaattctttt tttaaaaata caaaaatttt ttataaatta
     5821 tatagaccat gatattgtat taatactaat tgattttaat ttaaggttga actaaaataa
     5881 acatagtttt gtagaaaata atttttcatt attttgctac ttgatattgg atcgtaaaaa
     5941 tattagggct ttacaaagtt cttttaaaag agaatttagt aaagccctaa gtcttttaat
     6001 aaaaaatttt ttgtaaaaaa gttggcaaaa atagtttttg ctatatattt atatataaga
     6061 aaaaattcaa tactcaagga gataaaaaat ggaaaactta aacaataatc cacaaaaaat
     6121 taatcaaata aataagcaaa ctaattttat tggttttgaa aattttatag aacttgataa
     6181 tcaagttaat caaactaaag aaaaaagtaa attaagtaag ataggtaaga aattacccgg
     6241 aataagcagt caagtctagt gcggccgcgc ccaatacgca aaccgcctct ccccgcgcgt
     6301 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6361 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6421 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6481 tatgacatga ttacgaattc gagctcgaat tctaaagatc tttgacagct agctcagtcc
     6541 tagg
//
