LOCUS       sequence-311518-       10408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6622 MSCV-P C HA GLTSCR
FEATURES             Location/Qualifiers
     source          1..10408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1417..1436
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1417..1435
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1465..1489
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5474..5498)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5545..5571
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5545..5571
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5584..6083
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5619..5642)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6016..6035
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6104..6703
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6104..6123)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6600..6620
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6803..7317
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7479..7495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7503..7509,7510..7527,7528..7533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7548..7569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7686..7703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7857..8445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7937..7956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8616..9407,9408..9476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9239..9258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9477..9581)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9649..9667
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9705..9727)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9827..9846
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10040..10062
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10190..10209
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctaat acgactcact atagggagac
     1441 ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcagaagg gccgtcaagg
     1501 cccaccatgg cgctcaacac gcccacctcc cagctcctgg ccaagcaggt gcccgtcagc
     1561 ggctacctgg cctcggcggc tggcccctcg gagcccgtga cgctggcgtc ggccggtgtc
     1621 tcgccacagg gggctggcct ggtcatccag aagaacctct cggccgctgt ggccaccacg
     1681 ctcaatggga actctgtgtt cggaggcgcg ggggccgcct cggctcccac cgggacgccc
     1741 tcgggacagc cgctggcggt ggccccaggc ctcggctcgt cgccactggt cccggcgccc
     1801 aacgtgatcc tgcatcgcac acccacgccc atccagccca agcccgcggg ggtgctgccg
     1861 cccaagctct accagctgac gcccaagccg tttgcgcccg cgggcgccac gctcaccatc
     1921 cagggcgagc cgggggcgct cccgcagcag cccaaggccc cgcagaacct gacgttcatg
     1981 gcggcgggga aggcgggcca gaacgtggtg ctgtcgggct tccccgcgcc tgcgctgcaa
     2041 gcgaacgtct tcaagcagcc accggccacc accaccggag cggccccgcc gcagcccccc
     2101 ggggccctga gcaaacccat gagcgtccac ctcctgaacc aaggcagcag catcgtcatc
     2161 cccgcccagc acatgctgcc gggccagaac cagttcctac tgcctggcgc cccggcggtc
     2221 cagctcccgc agcagctctc agccctgccg gccaacgtgg gcgggcagat cctggcggcc
     2281 gctgcccccc acacaggtgg acagctcatc gcgaacccca tcctcacaaa ccagaacctg
     2341 gcgggcccac tgagcctggg ccccgtgttg gccccccact ccggggccca cagcgcgcac
     2401 atcctctccg ccgctcccat ccaggtgggc cagcctgcgc tcttccagat gcccgtgtcg
     2461 ctggcggcgg gcagcctgcc cacgcagagc cagccagcgc ccgccgggcc ggccgccacc
     2521 actgtcctcc agggggtcac cctgcccccc agcgccgtgg ccatgctcaa cacccccgac
     2581 ggcctggtgc agccggccac ccctgccgct gccaccgggg aggccgcgcc tgtcctcacg
     2641 gtgcagcctg ccccccaggc gccccccgcg gtcagcacac ccctgcccct gggcctccag
     2701 cagccgcagg cgcagcagcc cccgcaggcc cccaccccac aggccgccgc cccgcctcag
     2761 gccaccaccc cccagcccag ccctggcctg gcgtctagcc cggagaagat cgtcctgggg
     2821 cagccgccct ctgccacccc cacggccatc ctcactcagg actccctgca gatgttcctg
     2881 ccccaggaga ggagccagca gcccctctcc gcagagggcc cccacctctc cgtgcctgcc
     2941 tcggtcatag tcagcgcccc gcctcccgcc caagacccag ccccagccac ccccgtcgcc
     3001 aaaggagctg gcctcggccc tcaggccccc gacagccagg cttccccggc tccggccccc
     3061 cagatcccgg cagcggctcc gctgaagggc ccaggcccct cttcgtcccc gtcactacct
     3121 caccaggccc ctctggggga cagcccccac ctgccctccc cacaccccac ccggccccct
     3181 tcccgcccac cctcccggcc acagagtgtg tcccgccctc cctcagagcc acccttgcac
     3241 ccttgccccc caccccaggc ccccccaact ctgcctggca tctttgtcat ccaaaaccag
     3301 ctaggcgttc ccccgcctgc cagcaacccg gcccctactg ccccaggccc gccgcagccg
     3361 cctctccgcc cccagtccca gccgcctgag ggaccgctgc ccccagcccc ccacctccct
     3421 ccatcctcca cctcctctgc tgtggcctcc tcctctgaga cgtcctccag gttgccagcc
     3481 cctacgccat ccgacttcca gctccagttc ccacccagcc aggggcccca caagtccccc
     3541 actccccctc caaccctcca cctggtccct gagccggcag cacccccccc accgcctcct
     3601 cggaccttcc agatggtgac cacccccttc ccagcgctgc cccagccgaa ggctcttctc
     3661 gagagatttc accaggtgcc gtccggaatc atcctccaga acaaggctgg gggggcccct
     3721 gccgccccgc agacctccac cagcctgggg cccctcacca gccccgctgc gtctgtgctg
     3781 gtcagtgggc aggccccatc tgggaccccc actgccccca gccacgcccc cgccccggca
     3841 cccatggccg ccacaggcct ccctcctctg cttccagccg agaacaaggc ttttgccagc
     3901 aacctcccga ccctgaatgt ggccaaggcc gcttcctccg ggccagggaa gccctccggg
     3961 ctgcagtatg agagcaaact gagtggcctg aagaagcccc ccacgcttca gcccagcaag
     4021 gaagcctgtt tcctggagca tttgcacaaa caccagggct ccgtcctgca ccccgactac
     4081 aagacggcct tcccctcctt tgaggacgcc ctgcatcgcc tcctgcccta ccatgtctac
     4141 cagggcgccc tcccctcccc cagtgactac cacaaagtgg acgaggagtt tgagacggtc
     4201 tccacgcagc tgctgaaacg cacccaggcc atgctcaata aatatcggct cctgctcctg
     4261 gaggagtccc ggagggtgag cccctcagcg gagatggtaa tgatcgaccg aatgttcatt
     4321 caggaggaga agaccaccct tgccttggat aaacagctgg ccaaggagaa gccggacgag
     4381 tacgtgtctt cctcccgctc gctcggcctc cccatcgcag cctcttccga gggtcatcgg
     4441 cttcccggcc acggccccct gtcgtcttca gctcccgggg cctccaccca gccccctcca
     4501 cacctgccca ccaagcttgt gatccggcac ggcggggcag gcggctcccc ttcggtcacc
     4561 tgggcccggg cgtcctcctc cctgtcctcc tcttcctcct cctcctctgc cgcctcctcc
     4621 ttggacgccg acgaggacgg ccccatgccc tcccgcaacc gcccgcccat caagacctac
     4681 gaggcccgga gccgcatcgg gctcaagctc aagatcaagc aggaagccgg gctcagcaag
     4741 gtcgtgcaca acacggccct ggaccccgtg caccagcccc cgccaccccc cgctaccctc
     4801 aaggtggccg agcccccgcc acggccgcca ccaccaccgc cgcccacggg ccagatgaac
     4861 ggcacggtgg accacccgcc gcctgccgcc cccgagcgca agcccctggg caccgccccg
     4921 cactgcccgc gcctgccact gcgcaagacc taccgcgaga acgtgggggg ccctggcgcg
     4981 ccggagggga cgcccgcagg cagggcacgg ggaggcagcc cggcgccgct gcccgccaaa
     5041 gtggacgagg ccaccagcgg gctcatccgc gagctggcgg ccgtggagga cgagctgtac
     5101 cagcgtatgc tgaagggccc cccgccagag cccgcagcca gcgccgccca aggcaccggg
     5161 gaccccgact gggaggcgcc cgggctgccc cctgccaagc ggcgcaagtc cgagtcgccc
     5221 gacgtggacc aggccagctt ctccagcgac agcccgcagg atgacacgct caccgagcac
     5281 ctgcagagcg ccatcgacag catcctgaac ctgcagcagg cccccggccg gacgcccgcg
     5341 ccctcgtacc cccacgctgc ctcggccggc acccccgcat ccccgccgcc cctgcacagg
     5401 cccgaggcct acccaccctc cagtcacaac ggtggcctcg gcgccaggac gttgaccaga
     5461 tcaggcctca tgggcccagc tttcttgtac aaagtggttg atctagaggg cccgcggttc
     5521 gaactcgagt cggccgctgg aggatacccc tacgacgtgc ccgactacgc ctagaattct
     5581 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     5641 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     5701 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     5761 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     5821 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     5881 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     5941 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     6001 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     6061 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     6121 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6181 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6241 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6301 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6361 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6421 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6481 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6541 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6601 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6661 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     6721 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt
     6781 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     6841 agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca
     6901 aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt
     6961 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt
     7021 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     7081 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     7141 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg
     7201 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     7261 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg
     7321 taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat
     7381 cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat
     7441 tatggacagc gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca
     7501 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7561 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7621 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7681 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7741 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7921 aacccgacag gactataaag ataccaggcg tttccccttg gaagctccct cgtgcgctct
     7981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8281 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9601 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9661 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     9721 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     9781 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     9841 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     9901 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     9961 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    10021 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg
    10081 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
    10141 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
    10201 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
    10261 cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
    10321 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
    10381 ttttatttag tctccagaaa aagggggg
//
