LOCUS       sequence-31152-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Sears IB, O'Connor J, Rossanese OW, Glick BS
  TITLE     A versatile set of vectors for constitutive and regulated gene 
            expression in Pichia pastoris.
  JOURNAL   Yeast. 1998 Jun 15;14(8):783-90.
  PUBMED    9675822
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-06-15"; volume: "14(8)"; pages: "783-90"
COMMENT     Sequence Label: pIB2
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(111..587)
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     primer_bind     complement(610..629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     729..751
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(789..807)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccggtcttct cgtagtgccc aacttgaact gaggaacagt catgtctaag aagcttgcat
       61 gcctgcagct cgagactagt ggatccccgg gtaccgagct cgaattcttg atagttgttc
      121 aattgattga aatagggaca aataaattaa atttaaagtc tttgggtcag gagaaaccaa
      181 aattgggaaa ggtgttcgcc ttttatattc gattctggtg gtttccaata atctcatgac
      241 atgcgtccgc ccgctattat tgccagcgac ggccgggact tttccatccc tgggctgcta
      301 ggtcgggtac acgacctccg ttttacccgc aacgtaatgc tgggaagagc attgctgcaa
      361 gggggccgta gaagaagctc tccagcagag taaaatttcc tagggacggt aacgggcggt
      421 ggaaggagag agaagggaag agacgtttct ggttccatta ctccacattt aagttttacc
      481 ccggagaatt ttacgttgcc agcaggtcgt tcggagttgc aacggagcca gatatttcag
      541 agatggctac ctgattggac gaggacacca agacatttct acaaaaaagg atctatggtg
      601 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      661 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      721 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      781 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      841 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga a
//
