LOCUS       sequence-311537-        8500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conjugative vector derived from pSHDY. Constitutive expression of 
            the repressor vanR, as well as vanillate-inducible expression of 
            mVenus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 4553 to 4587)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY-PvanCC-mVenus_vanR
FEATURES             Location/Qualifiers
     source          1..8500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(124..963)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(1474..2445)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            3684..3770
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      4112..4506
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     promoter        4553..4587
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      5358..5385
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5417..6208)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVMHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6414..6435
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_RNA        6488..6537
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      7363..7434
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7450..7477
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(7530..7564)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_feature    7565..7585
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(7786..8500,1..137))
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
ORIGIN
        1 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg
       61 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg
      121 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg
      181 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt
      241 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc
      301 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag
      361 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt
      421 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg
      481 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag
      541 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc
      601 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac
      661 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc
      721 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc
      781 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc
      841 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta
      901 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc
      961 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg
     1021 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc
     1081 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg
     1141 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata
     1201 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt
     1261 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt
     1321 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta
     1381 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc
     1441 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct
     1501 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac
     1561 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg
     1621 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact
     1681 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc
     1741 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg
     1801 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg
     1861 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc
     1921 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg
     1981 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc
     2041 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc
     2101 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag
     2161 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac
     2221 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc
     2281 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt
     2341 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc
     2401 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcgc
     2461 agccagatcg agccgtcctc ggttgtcagt ggcgtcaggt cgagcaagag caacgatgcg
     2521 atcagcagca ccaccgtagg catcatggaa gccagcatca cggttagcca tagcttccag
     2581 tgccaccccc gcgacgcgct ccgggcgctc tgcgcggcgc tgctcacctc ggcggctacc
     2641 tcccgcaact ctttggccag ctccacccat gccgcccctg tctggcgctg ggctttcagc
     2701 cactccgccg cctgcgcctc gctggcctgc tgggtctggc tcatgacctg ccgggcttcg
     2761 tcggccagtg tcgccatgct ctgggccagc ggttcgatct gctccgctaa ctcgttgatg
     2821 cctctggatt tcttcactct gtcgattgcg ttcatggtct attgcctccc ggtattcctg
     2881 taagtcgatg atctgggcgt tggcggtgtc gatgttcagg gccacgtctg cccggtcggt
     2941 gcggatgccc cggccttcca tctccaccac gttcggcccc aggtgaacac cgggcaggcg
     3001 ctcgatgccc tgcgcctcaa gtgttctgtg gtcaatgcgg gcgtcgtggc cagcccgctc
     3061 taatgcccgg ttggcatggt cggcccatgc ctcgcgggtc tgctcaagcc atgccttggg
     3121 cttgagcgct tcggtcttct gtgccccgcc cttctccggg gtcttgccgt tgtaccgctt
     3181 gaaccactga gcggcgggcc gctcgatgcc gtcattgatc cgctcggaga tcatcaggtg
     3241 gcagtgcggg ttctcgccgc caccggcatg gatggccagc gtatacggca ggcgctcggc
     3301 accggtcagg tgctgggcga actcggacgc cagcgccttc tgctggtcga gggtcagctc
     3361 gaccggcagg gcaaattcga cctccttgaa cagccgccca ttggcgcgtt catacaggtc
     3421 ggcagcatcc cagtagtcgg cgggccgctc gacgaactcc ggcatgtgcc cggattcggc
     3481 gtgcaagact tcatccatgt cgcgggcata cttgccttcg cgctggatga agtcggcctt
     3541 ggccctggcc gattggccgc ccgacctgct gccggttttc gccgtaaggt gataaatcgc
     3601 catgctgcct cgctgttgct tttgcttttc ggctccatgc aatggccctc ggagagcgca
     3661 ccgcccgaag ggtggccgtt aggccagttt ctcgaagaga aaccggtaag tgcgcctccc
     3721 ctacaaagta gggtcgggat tgccgccgct gtgcctccat gatagcctac gagacagcac
     3781 attaacaatg gggtgtcaag atggttaagg ggagcaacaa ggcggcggat cggctggcca
     3841 agctcgaaga acaacgagcg cgaatcaatg ccgaaattca gcgggtgcgg gcaagggaac
     3901 agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct ggtgggggcc atgattttgg
     3961 ccaaggtgaa cagcagcgag tggccggagg atcggctcat ggcggcaatg gatgcgtacc
     4021 ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc acgccagaag gatgagccgg
     4081 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt
     4141 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg
     4201 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta
     4261 gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca
     4321 gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg
     4381 atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg
     4441 tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg
     4501 ggttcgtgac agttagtacg ggagtgacgg gggtaccgag gtcgatgtcg acttgacggc
     4561 tagctcagtc ctaggtacag tgctagcgct taaactaacg aacgtaaata aggaggatag
     4621 acatggacat gcctcgtatt aaaccgggtc agcgtgttat gatggcactg cgtaaaatga
     4681 ttgcaagcgg tgaaatcaaa agtggtgaac gtattgcaga aattccgacc gcagcagcac
     4741 tgggtgttag ccgtatgccg gttcgtatcg cactgcgttc actggaacaa gaaggtctgg
     4801 ttgttcgtct gggtgcacgt ggttatgcag cccgtggtgt tagcagcgat cagattcgtg
     4861 atgcaattga agttcgtggt gttctggaag gttttgcagc acgtcgtctg gcagaacgtg
     4921 gtatgaccgc agaaacccat gcacgttttg ttgtactgat tgcagaaggt gaagcactgt
     4981 ttgcagccgg tcgcctgaat ggtgaagatc tggatcgtta tgccgcatat aatcaggcat
     5041 ttcatgatac cctggttagc gcagcaggta atggtgcagt tgaaagcgca ctggcacgta
     5101 atggttttga accgtttgca gcagccggtg cactggccct ggatctgatg gacctgtctg
     5161 ccgaatatga acatctgctg gcagcacatc gtcagcatca ggcagttctg gatgcagtta
     5221 gctgtggtga tgccgaaggt gcagaacgta ttatgcgtga tcatgcactg gcagcaattc
     5281 gtaatgcaaa agtttttgaa gcagcagcaa gcgcaggcgc accgctgggt gcagcatggt
     5341 caattcgtgc agattgaggc tcaccttcgg gtgggccttt ctgcgtttat actcgagagc
     5401 ggccgaagct tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
     5461 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
     5521 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
     5581 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
     5641 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
     5701 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
     5761 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
     5821 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
     5881 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcataacaa
     5941 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
     6001 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
     6061 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
     6121 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
     6181 atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct
     6241 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
     6301 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga
     6361 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac caggaattcg
     6421 cggccgcttc tagagattgg atccaattga cagctagctc agtcctaggt accattggat
     6481 ccaatagctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac gaaacagcct
     6541 ctacaaataa ttttgtttaa tactagagta gtggaggtta ctagatggct agcaaaggag
     6601 aggaactgtt taccggtgtg gtacccattt tagtagaatt ggatggtgat gtgaacggcc
     6661 acaagttcag cgtttccggg gaaggcgaag gggatgcaac ctatggaaag ctaactttga
     6721 aactcatttg cactaccggt aaactccccg tcccctggcc tacgttagtc acgaccctcg
     6781 gttatggtct gcaatgtttt gcccgttatc ccgatcatat gaaacaacac gatttcttta
     6841 aatctgccat gccagaggga tatgttcaag aacggacaat tttctttaag gatgatggta
     6901 attataaaac ccgtgcagaa gtcaaatttg aaggtgatac actcgtgaat cgcatcgaac
     6961 taaaaggtat tgactttaag gaggatggaa acattctcgg ccataagtta gaatacaatt
     7021 acaacagcca taatgtgtac attactgcgg ataaacaaaa aaacggtatc aaagccaact
     7081 ttaaaattcg acataacatc gaagatggtg gcgttcagtt agcggatcac taccaacaaa
     7141 atactcccat tggagacgga ccagtcctgc tgcctgataa ccattatctg tcttatcaat
     7201 ccaaactgtc caaggatccg aatgaaaagc gggatcacat ggttctgttg gagtttgtga
     7261 ccgcggcagg tattactctc ggaatggacg aactatacaa aaggcctgcc gccaacgacg
     7321 aaaactacgc tgcatcagtt taatagtact agagccaggc atcaaataaa acgaaaggct
     7381 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     7441 agtcacactg gctcaccttc gggtgggcct ttctgcgtta ctagttcaat ctgcacgaat
     7501 tgaccttatt tacgttcgtt agtttaagcg ctagcattat acctaggact gagctagctg
     7561 tcaatactag tagcggccgc tgcaggagca gaagagcata catctggaag caaagccagg
     7621 aaagcggcct atggagctgt gcggcagcgc tcagtaggca atttttcaaa atattgttaa
     7681 gccttttctg agcatggtat ttttcatggt attaccaatt agcaggaaaa taagccattg
     7741 aatataaaag ataaaaatgt cttgtttaca atagagtggg gggggtcagc ctgccgcctt
     7801 gggccgggtg atgtcgtact tgcccgccgc gaactcggtt accgtccagc ccagcgcgac
     7861 cagctccggc aacgcctcgc gcacccgctg gcggcgcttg cgcatggtcg aaccactggc
     7921 ctctgacggc cagacatagc cgcacaaggt atctatggaa gccttgccgg ttttgccggg
     7981 gtcgatccag ccacacagcc gctggtgcag caggcgggcg gtttcgctgt ccagcgcccg
     8041 cacctcgtcc atgctgatgc gcacatgctg gccgccaccc atgacggcct gcgcgatcaa
     8101 ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg ctggcgtact ccgacagcag
     8161 ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg gataccttcc aaaggcgctc
     8221 gatgcagtcc tgtatgtgct tgagcgcccc accactatcg acctctgccc cgatttcctt
     8281 tgccagcgcc cgatagctac ctttgaccac catggcatca gcggtgacgg cctcccactt
     8341 gggttccagg aacagccgga gctgccgtcc gccttcggtc ttgggttccg ggccaagcac
     8401 taggccatta ggcccagcca tggccaccag cccttgcagg atgcgcagat catcagcgcc
     8461 cagcggctcc gggccgctga actcgatccg cttgccgtcg
//
