LOCUS       sequence-31154-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Sears IB, O'Connor J, Rossanese OW, Glick BS
  TITLE     A versatile set of vectors for constitutive and regulated gene 
            expression in Pichia pastoris.
  JOURNAL   Yeast. 1998 Jun 15;14(8):783-90.
  PUBMED    9675822
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-06-15"; volume: "14(8)"; pages: "783-90"
COMMENT     Sequence Label: pIB3
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(628..647)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(807..825)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccggtcttct cgtagtgccc aacttgaact gaggaacagt catgtctaag aagcttgcat
       61 gcctgcagct cgagactagt ggatccccgg gtaccgagct cgaattcgac tgctattatc
      121 tctgtgtgta tgtgtgtatt gggcaacaag agcttgtgaa tctgtcctta taaaagacac
      181 ccgaagaggc aagattgggt agtacactaa ttagtggagg gcaactggtt taggggtttt
      241 ggctggctta ttatagtgtc agcgatacta tacaatctac gatgcccgat gtcggcatac
      301 agcacattat tagatccaaa atttccttaa tttcctattc acgttatata tattaaccag
      361 aatttatccg ttgaattgct taatcgactt cttccacggt tggtccgctg tctccgcctg
      421 gggcagcacc acctggggca ccgccagctg cgccagctcc gccagggaaa cctccaggga
      481 agccaccagg agctccacca ggagctccac cagcagctcc atagaacttg ctcattattg
      541 ggttagcaac ttcttccaat tccttttgcc tgtccttgta ctcgtctgtg gaagcagatt
      601 gtgactcatc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      661 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
      721 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcanaggt tttcaccgtc
      781 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      841 catgataata atggtttctt aga
//
