LOCUS       sequence-311543-       10253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of the PULSE system under the control 
            of PAtUbi10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pROF141
FEATURES             Location/Qualifiers
     source          1..10253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             696..716
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1382..1634
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             3605..3625
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3652..3904
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             6922..7155
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             7156..7176
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7203..7455
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7692..7716
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7807..8395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7887..7906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8566..9381)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8566..9381)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9289..9308
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9373..9392
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9672..10107
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    join(10234..10253,1..3)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 taacaagctt cgtctcagtc aggaggtcga cgagtcagta ataaacggcg tcaaagtggt
       61 tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc
      121 taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt
      181 cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt
      241 cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt cacaattcag
      301 atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta
      361 aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt
      421 cgtatatgtt ctttggttta gattctgtta atcttagatc gaagacgatt ttctgggttt
      481 gatcgttaga tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc
      541 aaataatttg agttttgtcg aataattact cttcgatttg tgatttctat ctagatctgg
      601 tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt ctgattaaca
      661 gaatgcttga tcttgaactt agacttggtt ttgctccaaa gaagaagagg aaggtgggag
      721 ctggagctgg ggcagacgac acacgcgttg aggtgcaacc gccggcgcag tgggtcctcg
      781 acctgatcga ggccagcccg atcgcatcgg tcgtgtccga tccgcgtctt gccgacaatc
      841 cgctgatcgc catcaaccag gccttcaccg acctgaccgg ctattccgaa gaagaatgcg
      901 tcggccgcaa ttgccgattc ctggcaggtt ccggcaccga gccgtggctg accgacaaga
      961 tccgccaagg cgtgcgcgag cacaagccgg tgctggtcga gatcctgaac tacaagaagg
     1021 acggcacgcc gttccgcaat gccgtgctcg ttgcaccgat ctacgatgac gacgacgagc
     1081 ttctctattt cctcggcagc caggtcgaag tcgacgacga ccagcccaac atgggcatgg
     1141 cgcgccgcga acgcgccgcg gaaatgctca agacgctgtc gccgcgccag ctcgaggtta
     1201 cgacgctggt ggcatcgggc ttgcgcaaca aggaagtggc ggcccggctc ggcctgtcgg
     1261 agaaaaccgt caagatgcac cgcgggctgg tgatggaaaa gctcaacctg aagaccagtg
     1321 ccgatctggt gcgcattgcc gtcgaagccg gaatctaagc ttggaatgga tcttcgatcc
     1381 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     1441 gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     1501 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     1561 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     1621 tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc ctcgtgatac
     1681 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1741 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1801 atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga ctcgcgctgt
     1861 caggagtctc aatggtaact ttactcttta tttaaccata catttttttt tatttttttc
     1921 actttgttct tcatccacta ttgttctttg ttcatcttga acaaaagctc cctccttctt
     1981 tgttcttcat ccaccattgt tcttcatcaa tcatttcgct gtcaggaggt cgacgagtca
     2041 gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa
     2101 agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca actttgcgtg
     2161 taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc gccttagctt
     2221 tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca atacccaaag
     2281 agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa
     2341 ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa atcttcgatt
     2401 ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg ttaatcttag
     2461 atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg attagggttt
     2521 catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt actcttcgat
     2581 ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt
     2641 aatctgagtt tttctgatta acagaatgac aacaatgccc cgccccaagc tcaagtccga
     2701 tgacgaggta ctcgaggccg ccaccgtagt gctgaagcgt tgcggtccca tagagttcac
     2761 gctcagcgga gtagcaaagg aggtggggct ctcccgcgca gcgttaatcc agcgcttcac
     2821 caaccgcgat acgctgctgg tgaggatgat ggagcgcggc gtcgagcagg tgcggcatta
     2881 cctgaatgcg ataccgatag gcgcagggcc gcaagggctc tgggaatttt tgcaggtgct
     2941 cgttcggagc atgaacactc gcaacgactt ctcggtgaac tatctcatct cctggtacga
     3001 gctccaggtg ccggagctac gcacgcttgc gatccagcgg aaccgcgcgg tggtggaggg
     3061 gatccgcaag cgactgcccc caggtgctcc tgcggcagct gagttgctcc tgcactcggt
     3121 catcgctggc gcgacgatgc agtgggccgt cgatccggat ggtgagctag ctgatcatgt
     3181 gctggctcag atcgctgcca tcctgtgttt aatgtttccc gaacacgacg atttccaact
     3241 cctccaggca catgcgtccg cgtacagctt aattaacagt gctggtagtg ctggtagtgc
     3301 tggtatgatg ttcttaccaa ccgattattg ttgcaggtta agcgatcaag agtatatgga
     3361 gcttgtgttt gagaatggcc agattcttgc aaagggccaa agatccaacg tttctctgca
     3421 taatcaacgt accaaatcga tcatggattt gtatgaggca gagtataacg aggatttcat
     3481 gaagagtatc atccatggtg gtggtggtgc catcacaaat ctcggggaca cgcaggttgt
     3541 tccacaaagt catgttgctg ctgcccatga aacaaacatg ttggaaagca ataaacatgt
     3601 tgacccaaag aagaagagga aggtgtaggc ttggaatgga tcttcgatcc cgatcgttca
     3661 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc
     3721 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     3781 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     3841 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     3901 gatcgggaat tgccaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt
     3961 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4081 gagacaataa ccctgataaa tgcttcaata atgggaccga ctcgcgctgt caggaggtcg
     4141 acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat
     4201 caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac
     4261 tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc
     4321 cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat
     4381 acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt
     4441 tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt ctctcaaaat
     4501 cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt agattctgtt
     4561 aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt aattctcgat
     4621 tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc gaataattac
     4681 tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg cgatcgaatt
     4741 tgtcgattaa tctgagtttt tctgattaac agaatgacaa caatggtttc cggagtcggg
     4801 ggtagtggcg gtggccgtgg cggtggccgt ggcggagaag aagaaccgtc gtcaagtcac
     4861 actcctaata accgaagagg aggagaacaa gctcaatcgt cgggaacgaa atctctcaga
     4921 ccaagaagca acactgaatc aatgagcaaa gcaattcaac agtacaccgt cgacgcaaga
     4981 ctccacgccg ttttcgaaca atccggcgaa tcagggaaat cattcgacta ctcacaatca
     5041 ctcaaaacga cgacgtacgg ttcctctgta cctgagcaac agatcacagc ttatctctct
     5101 cgaatccagc gaggtggtta cattcagcct ttcggatgta tgatcgccgt cgatgaatcc
     5161 agtttccgga tcatcggtta cagtgaaaac gccagagaaa tgttagggat tatgcctcaa
     5221 tctgttccta ctcttgagaa acctgagatt ctagctatgg gaactgatgt gagatctttg
     5281 ttcacttctt cgagctcgat tctactcgag cgtgctttcg ttgctcgaga gattaccttg
     5341 ttaaatccgg tttggatcca ttccaagaat actggtaaac cgttttacgc cattcttcat
     5401 aggattgatg ttggtgttgt tattgattta gagccagcta gaactgaaga tcctgcgctt
     5461 tctattgctg gtgctgttca atcgcagaaa ctcgcggttc gtgcgatttc tcagttacag
     5521 gctcttcctg gtggagatat taagcttttg tgtgacactg tcgtggaaag tgtgagggac
     5581 ttgactggtt atgatcgtgt tatggtttat aagtttcatg aagatgagca tggagaagtt
     5641 gtagctgaga gtaaacgaga cgatttagag ccttatattg gactgcatta tcctgctact
     5701 gatattcctc aagcgtcaag gttcttgttt aagcagaacc gtgtccgaat gatagtagat
     5761 tgcaatgcca cacctgttct tgtggtccag gacgataggc taactcagtc tatgtgcttg
     5821 gttggttcta ctcttagggc tcctcatggt tgtcactctc agtatatggc taacatggga
     5881 tctattgcgt ctttagcaat ggcggttata atcaatggaa atgaagatga tgggagcaat
     5941 gtagctagtg gaagaagctc gatgaggctt tggggtttgg ttgtttgcca tcacacttct
     6001 tctcgctgca taccgtttcc gctaaggtat gcttgtgagt ttttgatgca ggctttcggt
     6061 ttacagttaa acatggaatt gcagttagct ttgcaaatgt cagagaaacg cgttttgaga
     6121 acgcagacac tgttatgtga tatgcttctg cgtgactcgc ctgctggaat tgttacacag
     6181 agtcccagta tcatggactt agtgaaatgt gacggtgcag catttcttta ccacgggaag
     6241 tattacccgt tgggtgttgc tcctagtgaa gttcagataa aagatgttgt ggagtggttg
     6301 cttgcgaatc atgcggattc aaccggatta agcactgata gtttaggcga tgcggggtat
     6361 cccggtgcag ctgcgttagg ggatgctgtg tgcggtatgg cagttgcata tatcacaaaa
     6421 agagactttc ttttttggtt tcgatctcac actgcgaaag aaatcaaatg gggaggcgct
     6481 aagcatcatc cggaggataa agatgatggg caacgaatgc atcctcgttc gtcctttcag
     6541 gcttttcttg aagttgttaa gagccggagt cagccatggg aaactgcgga aatggatgcg
     6601 attcactcgc tccagcttat tctgagagac tcttttaaag aatctgaggc ggctatgaac
     6661 tctaaagttg tggatggtgt ggttcagcca tgtagggata tggcggggga acaggggatt
     6721 gatgagttag gtgaattcga tagtgctggt agtgctggta gtgctggttc cgcgtacagc
     6781 cgcgcgcgta cgaaaaacaa ttacgggtct accatcgagg gcctgctcga tctcccggac
     6841 gacgacgccc ccgaagaggc ggggctggcg gctccgcgcc tgtcctttct ccccgcggga
     6901 cacacgcgca gactgtcgac ggcccccccg accgatgtca gcctggggga cgagctccac
     6961 ttagacggcg aggacgtggc gatggcgcat gccgacgcgc tagacgattt cgatctggac
     7021 atgttggggg acggggattc cccgggtccg ggatttaccc cccacgactc cgccccctac
     7081 ggcgctctgg atatggccga cttcgagttt gagcagatgt ttaccgatgc ccttggaatt
     7141 gacgagtacg gtgggccaaa gaagaagagg aaggtgtagg cttggaatgg atcttcgatc
     7201 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     7261 cgacgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     7321 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     7381 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     7441 ctatgttact agatcgggaa ttgccaagct aattcttgaa gacgaaaggg cctcgtgata
     7501 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7621 tatccgctca tgagacaata accctgataa atgcttcaat aatgggaccg actcgcgctt
     7681 gagacgaagc ttgacaggat atattggcgg gtaaactaag tcgctgtatg tgtttgtttg
     7741 agatctcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7981 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8221 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8281 agttaccttc ggaagaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8461 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8521 ttttaaatca atctaaagta tatatgtgta acattggtct agtgattaga aaaactcatc
     8581 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     8641 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     8701 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     8761 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     8821 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     8881 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg
     8941 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     9001 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     9061 gaatgctgtt ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     9121 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     9181 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     9241 gggcttccca tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca
     9301 tttataccca tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt
     9361 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     9421 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     9481 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
     9541 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
     9601 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
     9661 tcaggcgatc cccatccaac agcccgccgt cgagcgggct tttttatccc cggaagcctg
     9721 tggatagagg gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct tgattcacgg
     9781 ggctttccgg cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg tacgtgaaat
     9841 cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa tcaaaaaggc
     9901 acgtgagaac gctaatagcc ctttcagatc aacagcttgc aaacacccct cgctccggca
     9961 agtagttaca gcaagtagta tgttcaatta gcttttcaat tatgaatata tatatcaatt
    10021 attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc cgtggacaac
    10081 cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac ccggcggccg gccgcaacag
    10141 atcgttttat aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg
    10201 gcttctggat ttccgatccc cggaattaga tcttggcagg atatattgtg gtg
//
