LOCUS       sequence-311555-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PiggyBac-mediated integration of V5-tagged siRNA-resistant 
            EJC-binding deficient CASC3 1-480.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Gerbracht JV, Boehm V, Britto-Borges T, Kallabis S, Wiederstein JL, 
            Ciriello S, Aschemeier DU, Kruger M, Frese CK, Altmuller J, 
            Dieterich C, Gehring NH
  TITLE     CASC3 promotes transcriptome-wide activation of nonsense-mediated 
            decay by the exon junction complex.
  JOURNAL   Nucleic Acids Res. 2020 Jul 4. pii: 5867419. doi: 
            10.1093/nar/gkaa564.
  PUBMED    32621609
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Jul 4. pii: 5867419. doi: 10.1093/nar/gkaa564."
COMMENT     Sequence Label: PB-CuO-V5 CASC3 siRes 1-480 188/218-mut
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             1200..1241
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(2706..3280,3281..3283,3284..3292)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2873..2890)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3100..3119
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3329..3991
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        4112..4323
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4336..4604
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4632..4641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4644..4670
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4650..4676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4788..5396
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     CDS             5433..5486
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5493..6092
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5493..5512)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5989..6009
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6139..6260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6176..6195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6230..6249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7023..7057
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7548..7564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7548..7564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7561..7583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7572..7588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7596..7602,7603..7620,7621..7626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7641..7662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7779..7796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7950..8538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8030..8049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8709..9500,9501..9569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9332..9351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9570..9674)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9700..10155)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9837..9858)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10049..10068
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10282..10304
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10296..10313
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10297..10313
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10443..10505
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta atacccccgc cccgttgacg caaatgggca agcttgccgg
      481 gtcgaggtag gcgtgtacgg tgggaggcct atataagcaa ccggtataat caaacagacc
      541 agattgtctg tttgttaccg gtgtttagtg aaccgggcgc gcctcatatc gcctggagac
      601 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggtc
      661 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac
      721 tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtactcc
      781 gccaccgagg gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc
      841 gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg
      901 gtcggggttg tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag
      961 acggcggatg gtcgaggtga ggtgtggcag gcttgagatc cagctgttgg ggtgagtact
     1021 ccctctcaaa agcgggcatt acttctgcgc taagattgtc agtttccaaa aacgaggagg
     1081 atttgatatt cacctggccc gatctggcca tacacttgag tgacaatgac atccactttg
     1141 cctttctctc cacaggtgtc cactcccagg tccaagtttg gtctagagct agcaccatgg
     1201 gcaagcccat ccctaaccca ctgctgggcc tggacagcac cccacctctc gagatggcgg
     1261 accggcggcg gcagcgcgct tcgcaagaca ccgaggacga ggaatctggt gcttcgggct
     1321 ccgacagcgg cggctccccg ttgcggggag gcgggagctg cagcggtagc gccggaggcg
     1381 gcggcagcgg ctctctgcct tcacagcgcg gaggccgaac cggggccctt catctgcggc
     1441 gggtggagag cgggggcgcc aagagtgctg aggagtcgga gtgtgagagt gaagatggca
     1501 ttgaaggtga tgctgttctc tcggattatg aaagtgcaga agactcggaa ggtgaagaag
     1561 gtgaatacag tgaagaggaa aactccaaag tggagctgaa atcagaagct aatgatgctg
     1621 ttaattcttc aacaaaagaa gagaagggag aagaaaagcc tgacaccaaa agcactgtga
     1681 ctggagagag gcaaagtggg gacggacagg agagcacaga gcctgtggag aacaaagtgg
     1741 gtaaaaaggg ccctaagcat ttggatgatg atgaagatcg gaagaatcca gcatacatac
     1801 ctcggaaagg gctcgacttt gagcatgatc ttcgagggca aactcaggag gaggaagtca
     1861 gacccaaggg gcgtcagcga aagctatgga aggatgaggg tcgcgacgag catgacaagt
     1921 tccgggaaga tgagcaggcc ccaaagtccc gacaggagct cattgctctt tatggttatg
     1981 acattcgctc agctcataat ccagacgata ttaagccaag gcggatccgg aaaccccgat
     2041 atgggagtcc tccacaaaga gatccaaact ggaacggtga gcggctaaac aagtctcatc
     2101 gccaccaggg tcttgggggc accctaccac caaggacatt tattaacagg aatgctgcag
     2161 gtaccggccg tatgtctgca cccaggaatt attcccgatc tgggggcttc aaggaaggtc
     2221 gtgctggttt taggcctgtg gaagctggtg ggcagcatgg tggccggtct ggtgagactg
     2281 ttaagcatga gattagttac cggtcacggc gcctagagca gacttctgtg agggatccat
     2341 ctccagaagc agatgctcca gtgcttggca gtcctgagaa ggaagaggca gcctcagagc
     2401 caccagctgc tgctcctgat gctgcaccac caccccctga taggcccatt gagaagaaat
     2461 cctattcccg ggcaagaaga actcgaacca aagttggaga tgcagtcaag cttgcagagg
     2521 aggtgccccc tcctcctgaa ggactgattc cagcacctcc agtcccagaa accaccccaa
     2581 ctccacctac taagactggg acctgggaag ctccggtgga ttctagtaca agtggacttg
     2641 agcaagatgt ggcacaacta aatatagcag aacagaattg gagtccgggg cagtaagcgg
     2701 ccgcgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     2761 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     2821 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     2881 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     2941 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     3001 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     3061 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     3121 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     3181 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     3241 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggcgtc
     3301 cggaatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg
     3361 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg
     3421 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct
     3481 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa
     3541 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga
     3601 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg
     3661 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat
     3721 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg
     3781 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag
     3841 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt
     3901 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca
     3961 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc
     4021 cgtggacggc accgccggac ccggctccac cggatctcgc tagagctgaa tctaagtcga
     4081 cggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4141 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4201 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4261 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4321 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4381 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4441 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4501 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     4561 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     4621 cctactctag agccgccatg gcttacccat acgatgttcc agattacgct agtttggtga
     4681 ggcagcagag accgatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt
     4741 ccaagtttgg aagatctacc atgccaaaga gacccagacc cgtgatcatg agtccaaaga
     4801 gaagaacaca ggcagagcgc gcaatggaga cccagggcaa gttgattgca gcggccctgg
     4861 gggttttacg ggaaaaaggt tacgcgggat tccggatcgc agatgtgccc ggtgctgcag
     4921 gtgtctcgag aggagcgcag agccatcatt tcccgacaaa gcttgagctt ctgcttgcca
     4981 cttttgaatg gctttacgaa cagatcaccg aacgcagtcg ggctcgatta gcgaaattga
     5041 agccagagga tgacgtcatc cagcaaatgc tggacgacgc cgccgaattt ttcctcgacg
     5101 atgacttctc tatcagcctt gatttgattg tggctgccga ccgggatcca gcgttacgcg
     5161 agggtattca gcgcacggta gagaggaatc ggtttgtcgt cgaggatatg tggcttggtg
     5221 ttctggtgag ccgtggtctt tcgcgtgatg atgcagaaga tatcctttgg ttgatattca
     5281 attcggtgcg tgggcttgct gttcgtagcc tatggcagaa ggacaaagaa cgctttgagc
     5341 gtgtcaggaa ctcgacactc gaaattgcgc gagagcggta cgcgaaattc aagcgcgcgg
     5401 ccaaaccctc tggccggtct ctctctgttc gagagggcag aggaagtctt ctaacatgcg
     5461 gtgacgtgga ggagaatccc ggccctgaat tcatgaccga gtacaagccc acggtgcgcc
     5521 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     5581 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     5641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     5761 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     5821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     5881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5941 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6061 cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa
     6121 tttgtggtta actaactaaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6241 aaactcatca atgtatctta tcatgtctgg aattgactca aatgatgtca attagtctat
     6301 cagaagctca tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag
     6361 cagtgttccc aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc
     6421 ctgtcctcac aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat
     6481 tgactggatt ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg
     6541 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
     6601 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
     6661 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
     6721 ctgggggctt tggggggggg ctgtccctga tatctataac aagaaaatat atatataata
     6781 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     6841 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     6901 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     6961 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     7021 tgcatgcgtc aattttacgc agactatctt tctagggtta atctagctgc atcaggatca
     7081 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     7141 ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga
     7201 ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga
     7261 aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag
     7321 ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc
     7381 gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc
     7441 ggcgctggga tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac
     7501 atggataccc cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt
     7561 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7621 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7681 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7741 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7801 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7861 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7921 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7981 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8041 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8101 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8161 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8221 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8281 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8341 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8401 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8461 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8521 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8581 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8641 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8701 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8761 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8821 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8881 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8941 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9001 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9061 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9121 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9181 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9241 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9301 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9361 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9421 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9481 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9541 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9601 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9661 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     9721 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     9781 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     9841 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     9901 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     9961 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    10021 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    10081 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    10141 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10201 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10261 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
    10321 cctcgttcat tcacgttttt gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt
    10381 tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg cagctagatt
    10441 aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgtg taaaattgac
    10501 gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata gatattaagt
    10561 tttattatat ttacacttac atactaataa taaattcaac aaacaattta tttatgttta
    10621 tttatttatt aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttat
    10681 gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
    10741 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
    10801 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
    10861 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaggcctcca
    10921 cggccagact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    10981 ggagttccg
//
