LOCUS       sequence-311559-       10659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PiggyBac-mediated integration of V5-tagged siRNA-resistant 
            EJC-binding deficient CASC3 110-480 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Gerbracht JV, Boehm V, Britto-Borges T, Kallabis S, Wiederstein JL, 
            Ciriello S, Aschemeier DU, Kruger M, Frese CK, Altmuller J, 
            Dieterich C, Gehring NH
  TITLE     CASC3 promotes transcriptome-wide activation of nonsense-mediated 
            decay by the exon junction complex.
  JOURNAL   Nucleic Acids Res. 2020 Jul 4. pii: 5867419. doi: 
            10.1093/nar/gkaa564.
  PUBMED    32621609
REFERENCE   2  (bases 1 to 10659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Jul 4. pii: 5867419. doi: 10.1093/nar/gkaa564."
COMMENT     Sequence Label: PB-CuO-V5-CASC3 siRes 110-480 188/218-mut
FEATURES             Location/Qualifiers
     source          1..10659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             1200..1241
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(2376..2950,2951..2953,2954..2962)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2543..2560)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2770..2789
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2999..3661
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        3782..3993
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4006..4274
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4302..4311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4314..4340
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4320..4346
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4458..5066
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     CDS             5103..5156
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5163..5762
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5163..5182)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5659..5679
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5809..5930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5846..5865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5900..5919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6693..6727
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7218..7234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7218..7234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7231..7253)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7242..7258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7266..7272,7273..7290,7291..7296))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7311..7332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7449..7466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7620..8208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7700..7719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8379..9170,9171..9239))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9002..9021
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9240..9344)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9370..9825)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9507..9528)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9719..9738
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9952..9974
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9966..9983
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9967..9983
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10113..10175
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta atacccccgc cccgttgacg caaatgggca agcttgccgg
      481 gtcgaggtag gcgtgtacgg tgggaggcct atataagcaa ccggtataat caaacagacc
      541 agattgtctg tttgttaccg gtgtttagtg aaccgggcgc gcctcatatc gcctggagac
      601 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggtc
      661 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac
      721 tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtactcc
      781 gccaccgagg gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc
      841 gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg
      901 gtcggggttg tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag
      961 acggcggatg gtcgaggtga ggtgtggcag gcttgagatc cagctgttgg ggtgagtact
     1021 ccctctcaaa agcgggcatt acttctgcgc taagattgtc agtttccaaa aacgaggagg
     1081 atttgatatt cacctggccc gatctggcca tacacttgag tgacaatgac atccactttg
     1141 cctttctctc cacaggtgtc cactcccagg tccaagtttg gtctagagct agcaccatgg
     1201 gcaagcccat ccctaaccca ctgctgggcc tggacagcac cccacctctc gagtccaaag
     1261 tggagctgaa atcagaagct aatgatgctg ttaattcttc aacaaaagaa gagaagggag
     1321 aagaaaagcc tgacaccaaa agcactgtga ctggagagag gcaaagtggg gacggacagg
     1381 agagcacaga gcctgtggag aacaaagtgg gtaaaaaggg ccctaagcat ttggatgatg
     1441 atgaagatcg gaagaatcca gcatacatac ctcggaaagg gctcgacttt gagcatgatc
     1501 ttcgagggca aactcaggag gaggaagtca gacccaaggg gcgtcagcga aagctatgga
     1561 aggatgaggg tcgcgacgag catgacaagt tccgggaaga tgagcaggcc ccaaagtccc
     1621 gacaggagct cattgctctt tatggttatg acattcgctc agctcataat ccagacgata
     1681 ttaagccaag gcggatccgg aaaccccgat atgggagtcc tccacaaaga gatccaaact
     1741 ggaacggtga gcggctaaac aagtctcatc gccaccaggg tcttgggggc accctaccac
     1801 caaggacatt tattaacagg aatgctgcag gtaccggccg tatgtctgca cccaggaatt
     1861 attcccgatc tgggggcttc aaggaaggtc gtgctggttt taggcctgtg gaagctggtg
     1921 ggcagcatgg tggccggtct ggtgagactg ttaagcatga gattagttac cggtcacggc
     1981 gcctagagca gacttctgtg agggatccat ctccagaagc agatgctcca gtgcttggca
     2041 gtcctgagaa ggaagaggca gcctcagagc caccagctgc tgctcctgat gctgcaccac
     2101 caccccctga taggcccatt gagaagaaat cctattcccg ggcaagaaga actcgaacca
     2161 aagttggaga tgcagtcaag cttgcagagg aggtgccccc tcctcctgaa ggactgattc
     2221 cagcacctcc agtcccagaa accaccccaa ctccacctac taagactggg acctgggaag
     2281 ctccggtgga ttctagtaca agtggacttg agcaagatgt ggcacaacta aatatagcag
     2341 aacagaattg gagtccgggg cagtaagcgg ccgcgcccct ctccctcccc cccccctaac
     2401 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     2461 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     2521 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     2581 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     2641 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     2701 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     2761 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     2821 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     2881 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     2941 cgatgataat atggccacaa ccatggcgtc cggaatggag agcgacgaga gcggcctgcc
     3001 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     3061 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
     3121 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     3181 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     3241 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     3301 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
     3361 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     3421 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
     3481 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     3541 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
     3601 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc
     3661 cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac
     3721 cggatctcgc tagagctgaa tctaagtcga cggatctgcg atcgctccgg tgcccgtcag
     3781 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     3841 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     3901 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     3961 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     4021 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     4081 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     4141 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     4201 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     4261 cgccgttaca gatccaagct gtgaccggcg cctactctag agccgccatg gcttacccat
     4321 acgatgttcc agattacgct agtttggtga ggcagcagag accgatgaca tccactttgc
     4381 ctttctctcc acaggtgtcc actcccaggt ccaagtttgg aagatctacc atgccaaaga
     4441 gacccagacc cgtgatcatg agtccaaaga gaagaacaca ggcagagcgc gcaatggaga
     4501 cccagggcaa gttgattgca gcggccctgg gggttttacg ggaaaaaggt tacgcgggat
     4561 tccggatcgc agatgtgccc ggtgctgcag gtgtctcgag aggagcgcag agccatcatt
     4621 tcccgacaaa gcttgagctt ctgcttgcca cttttgaatg gctttacgaa cagatcaccg
     4681 aacgcagtcg ggctcgatta gcgaaattga agccagagga tgacgtcatc cagcaaatgc
     4741 tggacgacgc cgccgaattt ttcctcgacg atgacttctc tatcagcctt gatttgattg
     4801 tggctgccga ccgggatcca gcgttacgcg agggtattca gcgcacggta gagaggaatc
     4861 ggtttgtcgt cgaggatatg tggcttggtg ttctggtgag ccgtggtctt tcgcgtgatg
     4921 atgcagaaga tatcctttgg ttgatattca attcggtgcg tgggcttgct gttcgtagcc
     4981 tatggcagaa ggacaaagaa cgctttgagc gtgtcaggaa ctcgacactc gaaattgcgc
     5041 gagagcggta cgcgaaattc aagcgcgcgg ccaaaccctc tggccggtct ctctctgttc
     5101 gagagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctgaat
     5161 tcatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5221 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5281 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5341 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5401 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5461 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5521 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5581 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5641 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5701 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5761 gagtcgacaa tcaacctctg gattacaaaa tttgtggtta actaactaaa cttgtttatt
     5821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5941 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     6001 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     6061 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     6121 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     6181 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     6241 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     6301 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     6361 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     6421 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     6481 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     6541 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     6601 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     6661 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     6721 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     6781 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     6841 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     6901 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     6961 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     7021 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     7081 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     7141 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     7201 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7261 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7321 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7381 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7441 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7501 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7561 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8341 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8401 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8461 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8521 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8581 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8641 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8701 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8761 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8821 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8881 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8941 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9001 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9061 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9361 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     9421 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     9481 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     9541 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     9601 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     9661 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     9721 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     9781 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     9841 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9901 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9961 gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt gaacccgtgg
    10021 aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag atgaagatgc
    10081 tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat attgtgacgt
    10141 acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
    10201 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
    10261 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca aaaactcaaa
    10321 atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa agcccccagg
    10381 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
    10441 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
    10501 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
    10561 acctgggggg atacggggaa aaggcctcca cggccagact agttattaat agtaatcaat
    10621 tacggggtca ttagttcata gcccatatat ggagttccg
//
