LOCUS       sequence-311567-       10192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent redox reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10192)
  TITLE     Plasmids expressing redox reporters coupled to fluorescent protein 
            mCherry for lentiviral approaches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti6.2_mCherry_ mito_roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..10192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(804..806,807..1517)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(846..867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1107..1126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1454..1475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2115..2168
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2169..2178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2181..2190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2187..2897
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2332..2350)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2600..2619
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2912..3500
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2965..2985)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3383..3394)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3514..4013
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3549..3572)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3946..3965
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4034..4633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4034..4053)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4530..4550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4771..5004
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5076..5197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5113..5132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5167..5186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5237..5372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5392..5411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5393..5411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5421..5438)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5421..5437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5430..5452)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5579..6034
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5666..5685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5876..5897
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6060..6164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6165..6233,6234..7025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6383..6402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7196..7784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7685..7704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7938..7955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8072..8093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8108..8113,8114..8131,8132..8138)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8146..8162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8151..8173
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8170..8186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8170..8186
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8205..8225
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8207..8225
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8253..8479
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8480..8660
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8707..8832
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9325..9558
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9743..9787
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10054..10170
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagagatatc atggcctcca
      601 ctcgtgtcct cgcctctcgc ctggcctccc agatggctgc ttccgccaag gttgcccgcc
      661 ctgctgtccg cgttgctcag gtcagcaagc gcaccatcca gactggctcc cccctccaga
      721 ccctcaagcg cacccagatg acctccatcg tcaacgccac cacccgccag gctttccaga
      781 agcgcgccta ctcttccgga tccgtgagca agggcgagga gctgttcacc ggggtggtgc
      841 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      901 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctccacc accggcaagc
      961 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1021 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1081 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1141 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1201 acggcaacat cctggggcac aagctggagt acaactacaa ctgccacaac gtctatatca
     1261 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1321 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1381 tgctgctgcc cgacaaccac tacctgagca cctgctccgc cctgagcaaa gaccccaacg
     1441 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1501 tggacgagct gtacaagact agtggtggtt caggtggtgg tggttcaggt ggtggtggtt
     1561 caggtggagg aggatcagga ggaggaggat caggaggagg aggatcagga ggagaattcg
     1621 atatctcaga attctataag ctagcacctg ttgacaagaa aggccaacca ttccccttcg
     1681 accaattaaa gggaaaagtg gtgcttatcg ttaatgttgc ctccaaatgt ggattcactc
     1741 ctcaatacaa agaactagag gccttgtaca aacgttataa ggacgaagga tttaccatca
     1801 tcgggttccc atgcaaccag tttggccacc aagaacctgg ctctgatgaa gaaattgccc
     1861 agttctgcca actgaactat ggcgtgactt tccccattat gaaaaaaatt gacgttaatg
     1921 gtggcaatga ggaccctgtt tacaagtttt tgaagagcca aaaatccggt atgttgggct
     1981 tgagaggtat caaatggaat tttgaaaaat tcttagtcga taaaaagggt aaagtgtacg
     2041 aaagatactc ttcactaacc aaaccttctt cgttgtccga aaccatcgaa gaacttttga
     2101 aagaggtgga aaccgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     2161 ccggccctcc atggtggcgc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
     2221 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     2281 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     2341 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     2401 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     2461 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     2521 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     2581 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     2641 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     2701 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     2761 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     2821 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     2881 acgagctgta caagtaggcg gccgcgtcga caatcaacct ctggattaca aaatttgtga
     2941 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3001 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3061 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3121 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3181 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3241 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3301 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3361 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3421 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3481 cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc
     3541 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     3601 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     3661 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     3721 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     3781 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     3841 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     3901 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     3961 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     4021 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4081 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4141 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     4201 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4261 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4321 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4381 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4441 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4501 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4561 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4621 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     4681 ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4741 ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac
     4801 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4861 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4921 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4981 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     5041 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     5101 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5161 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5221 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5281 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5341 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     5401 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5461 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5521 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     5581 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5641 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5701 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5761 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5821 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5881 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5941 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6001 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     6061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7321 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7981 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8041 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8101 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8161 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8221 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     8281 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     8341 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     8401 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     8461 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     8521 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8581 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8641 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8701 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     8761 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     8821 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8881 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8941 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9001 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9061 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9121 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     9181 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9241 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9301 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9361 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9421 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9481 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9541 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9601 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9661 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9721 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9781 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9841 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9901 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9961 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    10021 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
    10081 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    10141 acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc cg
//
