LOCUS       sequence-311570-       10746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10746)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-AAK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..10746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..2858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2868..2892
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5515..5539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5613..5842
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5825..5844
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5915..6489,6490..6492,6493..6501)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6082..6099)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6309..6328
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6521..7120
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6521..6540)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7017..7037
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7135..7723
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7188..7208)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7606..7617)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7798..8031
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8150..8168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8236..8340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8341..8409,8410..9201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8559..8578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9372..9960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9861..9880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10114..10131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10372..10393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10408..10413,10414..10431,10432..10438)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10446..10462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10451..10473
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10470..10486
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10470..10486
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10536..10555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccat accggtcagc
     2821 tcgaggccac catggactac aaggacgacg acgacaaggg cggatcaaca agtttgtaca
     2881 aaaaagcagg ctccgaattc gcccttatga agaagttttt cgactcccgg cgagagcagg
     2941 gcggctctgg cctgggctcc ggctccagcg gaggaggggg cagcacctcg ggcctgggca
     3001 gtggctacat cggaagagtc ttcggcatcg ggcgacagca ggtcacagtg gacgaggtgt
     3061 tggcggaagg tggatttgct attgtatttc tggtgaggac aagcaatggg atgaaatgtg
     3121 ccttgaaacg catgtttgtc aacaatgagc atgatctcca ggtgtgcaag agagaaatcc
     3181 agataatgag ggatctttca gggcacaaga atattgtggg ttacattgat tctagtatca
     3241 acaacgtgag tagcggtgat gtatgggaag tgctcattct gatggacttt tgtagaggtg
     3301 gccaggtggt aaacctgatg aaccagcgcc tgcaaacagg ctttacagag aatgaagtgc
     3361 tccagatatt ttgtgatacc tgtgaagctg ttgcccgcct gcatcagtgc aaaactccta
     3421 ttatccaccg ggacctgaag gttgaaaaca tcctcttgca tgaccgaggc cactatgtcc
     3481 tgtgtgactt tggaagcgcc accaacaaat tccagaatcc acaaactgag ggagtcaatg
     3541 cagtagaaga tgagattaag aaatacacaa cgctgtccta tcgagcacca gaaatggtca
     3601 acctgtacag tggcaaaatc atcactacga aggcagacat ttgggctctt ggatgtttgt
     3661 tgtataaatt atgctacttc actttgccat ttggggaaag tcaggtggca atttgtgatg
     3721 gaaacttcac aattcctgat aattctcgat attctcaaga catgcactgc ctaattaggt
     3781 atatgttgga accagaccct gacaaaaggc cggatattta ccaggtgtcc tacttctcat
     3841 ttaagctact caagaaagag tgcccaattc caaatgtaca gaactctccc attcctgcaa
     3901 agcttcctga accagtgaaa gccagtgagg cagctgcaaa aaagacccag ccaaaggcca
     3961 gactgacaga tcccattccc accacagaga cttcaattgc accccgccag aggcctaaag
     4021 ctgggcagac tcagccgaac ccaggaatcc ttcccatcca gccagcgctg acaccccgga
     4081 agagggccac tgttcagccc ccacctcagg ctgcaggatc cagcaatcag cctggccttt
     4141 tagccagtgt tccccaacca aaaccccaag ccccacccag ccagcctctg ccgcaaactc
     4201 aggccaagca gccacaggct cctcccactc cacagcagac gccttctact caggcccagg
     4261 gtctgcccgc tcaggcccag gccacacccc agcaccagca gcaactcttc ctcaagcagc
     4321 aacagcagca gcaacagcca ccgccagcac agcagcagcc ggcaggcacg ttttaccagc
     4381 agcagcaggc ccagactcag cagtttcagg cagtacatcc agcaacccag caaccagcaa
     4441 ttgctcagtt ccctgtggtg tcccaaggag gctctcaaca gcagctaatg cagaatttct
     4501 accagcagca gcagcagcag caacaacaac agcaacagca acagctggcc acagccctgc
     4561 atcaacaaca gctgatgact cagcaggctg ccttgcagca aaagcccact atggcagcag
     4621 gacagcagcc ccagccacag ccagctgcag ccccacagcc agcccctgcc caggagccag
     4681 cgattcaagc cccagtaaga caacagccaa aggttcagac aaccccacct cctgccgtcc
     4741 aggggcagaa agttggatct ctcactccac cctcatcccc caaaacccaa cgtgctgggc
     4801 acaggcgtat tctcagtgac gtaacccaca gtgcagtctt tggggtccct gccagcaaat
     4861 caacccagct gctccaggca gctgcagctg aggccagtct caataggtcc aagtctgcaa
     4921 ccactactcc atcaggctct cctcggacct ctcaacaaaa cgtttataat ccttcagaag
     4981 ggtctacgtg gaatcccttt gatgacgata atttctccaa actcacagct gaagaactgc
     5041 taaacaagga ctttgccaag cttggggaag gcaaacatcc cgagaagctt ggaggctcag
     5101 ctgagagttt gatcccaggc tttcaatcaa cccaaggtga tgcttttgct acgacctcat
     5161 tttctgctgg aactgctgaa aaaaggaagg gtgggcagac tgtggactct ggcctcccgc
     5221 ttctaagcgt gtctgatcct ttcattcctc ttcaagtacc tgatgcacca gaaaaactaa
     5281 ttgagggact caagtctcct gacacttctc ttctgctccc tgacctcttg cctatgacag
     5341 atccttttgg tagcacttct gatgctgtaa ttggtaaagt catcatctct gtttcttcag
     5401 tcatgcatga tatgtgtgcc tgtttcaaga atgacaagta cctagttaac caatccctgg
     5461 ggaatagccc tgccacccca gaagccaagg ctattgaatt caagggcgaa ttcgacccag
     5521 ctttcttgta caaagtggtt gattaagatc cactagtaac ggccgccagt gtgctggaat
     5581 taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     5641 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     5701 cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt
     5761 gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc
     5821 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca
     5881 tctagggcgg ccaattccgc ccctctcccc cccccccctc tccctccccc ccccctaacg
     5941 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     6001 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     6061 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     6121 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     6181 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     6241 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     6301 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     6361 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     6421 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     6481 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac
     6541 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     6601 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     6661 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6721 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6781 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6841 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6901 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6961 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7021 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7081 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tatcgataga tcctaatcaa
     7141 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7201 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7261 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7321 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7381 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7441 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7501 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7561 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7621 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7681 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     7741 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7801 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7861 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7921 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7981 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8041 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8101 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     8161 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8221 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8281 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8341 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8401 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8461 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8521 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8581 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8641 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8701 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8761 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8821 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8881 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8941 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9001 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9061 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9121 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9181 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9241 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9301 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9481 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9601 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9661 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9721 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9781 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9841 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9901 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9961 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10021 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10081 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10141 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
    10201 ctcatggctg actaattttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10261 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
    10321 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10381 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10441 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
    10501 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10561 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    10621 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    10681 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    10741 agtagt
//
