LOCUS       sequence-311572-       10133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5-miniTurbo for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10133)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..10133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1909..1926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2043..2064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2079..2084,2085..2102,2103..2109)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2117..2133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2122..2144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2141..2157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2141..2157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2176..2196
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2178..2196
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2224..2450
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2451..2631
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2678..2803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3296..3529
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3714..3758
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4025..4141
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4173..4193
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4193..5404
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5241..5260
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5405..5429
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5432..6115
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(6117..6141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6143..6184
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6191..6961
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(6967..6987)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7016..7604
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7069..7089)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7487..7498)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7618..8117
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7653..7676)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8050..8069
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8138..8737
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8138..8157)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8634..8654
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8875..9108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9180..9301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9217..9236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9271..9290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9341..9476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9496..9515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9497..9515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9525..9542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9525..9541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9534..9556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9683..10111
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9770..9789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9980..10001
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1861 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1921 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1981 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     2041 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     2101 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     2161 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     2221 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     2281 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     2341 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     2401 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     2461 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     2521 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     2581 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     2641 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     2701 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     2761 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     2821 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2881 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2941 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     3001 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     3061 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     3121 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     3181 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     3241 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     3301 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     3361 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     3421 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     3481 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     3541 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     3601 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     3661 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     3721 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     3781 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3841 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3901 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     3961 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     4021 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     4081 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
     4141 tatcgtccag tgtggtggaa ttctgcagat atcaactttg tatagaaaag ttggcctccg
     4201 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     4261 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     4321 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     4381 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     4441 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     4501 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     4561 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     4621 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     4681 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     4741 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     4801 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     4861 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     4921 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     4981 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     5041 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     5101 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     5161 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     5221 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     5281 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     5341 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     5401 agacgcaact ttgtacaaaa aagttggcac catggtgagc ggcctgctga aggagagtat
     5461 gcgcatcaag atgtacatgg agggcaccgt gaacggccac tacttcaagt gcgagggcga
     5521 gggcgacggc aaccccttcg ccggcaccca gagcatgaga atccacgtga ccgagggcgc
     5581 ccccctgccc ttcgccttcg acatcctggc cccctgctgc gagtacggca gcaggacctt
     5641 cgtgcaccac accgccgaga tccccgactt cttcaagcag agcttccccg agggcttcac
     5701 ctgggagaga accaccacct acgaggacgg cggcatcctg accgcccacc aggacaccag
     5761 cctggagggc aactgcctga tctacaaggt gaaggtgcac ggcaccaact tccccgccga
     5821 cggccccgtg atgaagaaca agagcggcgg ctgggagccc agcaccgagg tggtgtaccc
     5881 cgagaacggc gtgctgtgcg gccggaacgt gatggccctg aaggtgggcg accggcacct
     5941 gatctgccac cactacacca gctaccggag caagaaggcc gtgcgcgccc tgaccatgcc
     6001 cggcttccac ttcaccgaca tccggctcca gatgctgcgg aagaagaagg acgagtactt
     6061 cgagctgtac gaggccagcg tggcccggta cagcgacctg cccgagaagg ccaacttgcc
     6121 aactttcttg tacaaagtgg gcggcaagcc catccccaac cccctgctgg gcctggacag
     6181 caccgctagc atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag
     6241 cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga
     6301 gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg
     6361 gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa
     6421 gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct
     6481 gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga
     6541 tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat
     6601 cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca
     6661 gggctggatc acactgcagg aagcagggat taacctggac aggaatactc tggccgctat
     6721 gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata
     6781 tctgtcacgg tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg
     6841 ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga
     6901 acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa
     6961 gtaagccaac tttattatac atagttgata tccagcacag tggcggccgc tcgacaatca
     7021 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7081 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7141 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7201 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7261 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7321 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7381 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7441 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7501 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7561 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctaccggg
     7621 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     7681 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     7741 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     7801 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     7861 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     7921 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     7981 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     8041 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     8101 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     8161 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8221 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8281 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8341 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8401 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8461 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8521 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8581 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     8641 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8701 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     8761 cccgaccgaa aggagcgcac gaccccatgc atctcgaggg cccggtacct ttaagaccaa
     8821 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8881 ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8941 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9001 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9061 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     9121 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     9181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9301 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     9361 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9421 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9481 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     9541 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     9601 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9661 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9721 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9781 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9841 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9901 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9961 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    10021 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    10081 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tac
//
