LOCUS       sequence-311573-       10015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent redox reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10015)
  TITLE     Plasmids expressing redox reporters coupled to fluorescent protein 
            mCherry for lentiviral approaches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti6.2_mCherry_mito Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..10015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1221..1223,1224..1934)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1263..1284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1524..1543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1871..1892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1938..1991
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1992..2001
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2004..2013
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2010..2720
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2155..2173)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2423..2442
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2735..3323
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2788..2808)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3206..3217)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3337..3836
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3372..3395)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3769..3788
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3857..4456
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3857..3876)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4353..4373
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4594..4827
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4899..5020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4936..4955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4990..5009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5060..5195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5215..5234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5216..5234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5244..5261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5244..5260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5253..5275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5402..5857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5489..5508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5699..5720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5883..5987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5988..6056,6057..6848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6206..6225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7019..7607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7508..7527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7761..7778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7895..7916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7931..7936,7937..7954,7955..7961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7969..7985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7974..7996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7993..8009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7993..8009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8028..8048
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8030..8048
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8076..8302
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8303..8483
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8530..8655
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9148..9381
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9566..9610
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9877..9993
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagagatatc atggcctcca
      601 ctcgtgtcct cgcctctcgc ctggcctccc agatggctgc ttccgccaag gttgcccgcc
      661 ctgctgtccg cgttgctcag gtcagcaagc gcaccatcca gactggctcc cccctccaga
      721 ccctcaagcg cacccagatg acctccatcg tcaacgccac cacccgccag gctttccaga
      781 agcgcgccta ctcttccgga tccgctcaag agtttgtgaa ctgcaaaatc cagcctggga
      841 aggtggttgt gttcatcaag cccacctgcc cgtactgcag gagggcccaa gagatcctca
      901 gtcaattgcc catcaaacaa gggcttctgg aatttgtcga tatcacagcc accaaccaca
      961 ctaacgagat tcaagattat ttgcaacagc tcacgggagc aagaacggtg cctcgagtct
     1021 ttattggtaa agattgtata ggcggatgca gtgatctagt ctctttgcaa cagagtgggg
     1081 aactgctgac gcggctaaag cagattggag ctctgcagac tagtggtggt tcaggtggtg
     1141 gtggttcagg tggtggtggt tcaggtggag gaggatcagg aggaggagga tcaggaggag
     1201 gaggatcagg aggagaattc gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1261 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1321 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctccaccacc ggcaagctgc
     1381 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1441 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1501 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1561 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1621 gcaacatcct ggggcacaag ctggagtaca actacaactg ccacaacgtc tatatcatgg
     1681 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1741 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1801 tgctgcccga caaccactac ctgagcacct gctccgccct gagcaaagac cccaacgaga
     1861 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1921 acgagctgta caagaccgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     1981 atcccggccc tccatggtgg cgcgccacca tggtgagcaa gggcgaggag gataacatgg
     2041 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     2101 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     2161 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     2221 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     2281 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2341 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     2401 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2461 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2521 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2581 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2641 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2701 tggacgagct gtacaagtag gcggccgcgt cgacaatcaa cctctggatt acaaaatttg
     2761 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2821 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2881 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2941 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3001 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3061 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3121 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     3181 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3241 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3301 ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa
     3361 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3421 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3481 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3541 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     3601 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     3661 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     3721 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     3781 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     3841 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     3901 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     3961 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4021 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4081 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     4141 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4201 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     4261 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     4321 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     4381 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     4441 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     4501 accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
     4561 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
     4621 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4681 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4741 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4801 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     4861 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5041 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5101 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5161 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     5221 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5281 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5341 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5401 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5461 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5521 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5581 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5641 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5701 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5761 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5821 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5881 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5941 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6001 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6061 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6121 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6181 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6241 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6301 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6361 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6421 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6481 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6541 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6601 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6661 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6721 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6781 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6841 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6901 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7141 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7681 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7741 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7801 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7861 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7921 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7981 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8041 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     8101 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     8161 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     8221 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     8281 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     8341 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8401 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8461 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     8521 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     8581 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     8641 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8701 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8761 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8821 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8881 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8941 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     9001 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     9061 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     9121 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     9181 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     9241 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     9301 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     9361 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     9421 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     9481 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     9541 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9601 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9661 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9721 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9781 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9841 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     9901 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9961 attacaaaaa caaattacaa aattcaaaat tttatcgata agcttgggag ttccg
//
