LOCUS       sequence-31159-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Losev E, Reinke CA, Jellen J, Strongin DE, Bevis BJ, Glick BS
  TITLE     Golgi maturation visualized in living yeast.
  JOURNAL   Nature. 2006 Jun 22. 441(7096):1002-6.
  PUBMED    16699524
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jun 22. 441(7096):1002-6."
COMMENT     Sequence Label: YCPlac33-sGFP-VRG4
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(521..537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(521..537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(534..556)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    545..561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(569..575,576..593,594..599))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    614..635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(752..769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttctccttta cccntgctca ttcgggcgaa agatactgtg tggttagtgt gtttgttttc
       61 ttaggctctt ttggtaatta agaggatgtc aacaattaat cggcaacaag aaataggctt
      121 ggcagcgtta tatatattct gtttttctgt tcattgtttg ttcggatcgc gaaatttcaa
      181 tttttttctt tctttttttt tttttcggcc aaaactatta tccaaggctg ggtaagagga
      241 ttctgaaaaa cccgtttagc ttttcgtata ttgcgattca ttacgcgttt aggcgacgtg
      301 tcttctttcg tcaactgtga tatttttcgt gttatgggcg gctaattctt tataaaaagg
      361 gaaatttaat cattatggca aactcaaatg gtgttcctac atctatatac caccatgcca
      421 ctttgtacca ccagtcatat attttcttct actcataagg gagccgcata tatatatggc
      481 gcacattgcc atggtccatt acaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      541 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      601 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      721 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      781 ttcggctgcg gcgagcggta tcanctcact caaaggcggt aatacggtta tccacagaat
      841 caggggataa cgcaggaaag a
//
