LOCUS       sequence-311593-        8735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing mCherry driven by human EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 8735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV-EF1amCherry-W
FEATURES             Location/Qualifiers
     source          1..8735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    166..291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    788..1021
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1206..1250
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1548..1665
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2136..3317
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2367..3309
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3266..3286
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3337..4047
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3482..3500)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3750..3769
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4113..4133)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4531..4542)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4994..5011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5000..5224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5250..5270)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5250..5268)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5289..5305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5289..5305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5302..5324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5313..5329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5337..5343,5344..5361,5362..5367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5382..5403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5520..5537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5691..6279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5771..5790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6450..7241,7242..7310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7073..7092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7311..7415)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7441..7896)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7578..7599)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7790..7809
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8023..8045
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8037..8054
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8038..8054
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8064..8083
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8064..8082
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        8153..8456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8458..8656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8611..8631
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             join(8674..8735,1..119)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
       61 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      121 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
      181 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
      241 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
      301 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
      361 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      421 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      481 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      541 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      601 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      661 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      721 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      781 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      841 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      901 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      961 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1021 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1081 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     1141 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     1201 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     1261 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1321 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1381 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1441 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1501 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1561 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1621 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1681 gacagcagag atccagtttg gttagtaccg ggccctacgc gtgaggggcg cggaagccgg
     1741 ggcggaggag gagccaaccc cgggctgggc tgagacccgc agaggaagac gctctaggga
     1801 tttgtcccgg actagcgaga tggcaaggct gaggacggga ggctgattga gaggcgaagg
     1861 tacaccctaa tctcaataca acctttggag ctaagccagc aatggtagag ggaagattct
     1921 gcacgtccct tccaggcggc ctccccgtca ccaccccccc caacccgccc cgaccggagc
     1981 tgagagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt
     2041 aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct
     2101 ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag
     2161 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     2221 cctagagaaa gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     2281 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     2341 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     2401 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg gctgcagtac
     2461 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     2521 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     2581 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     2641 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     2701 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     2761 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     2821 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     2881 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     2941 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3001 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3061 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3121 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3181 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     3241 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     3301 ccatttcagg tgtcgtggaa gcttggcgcg cccaccatgg tgagcaaggg cgaggaggat
     3361 aactccgcca tcatcaagga gttcctgcgc ttcaaggtgc acatggaggg ctccgtgaac
     3421 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3481 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     3541 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     3601 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     3661 gtggtgaccg tgacccagga ctcctctctg caggacggcg agttcatcta caaggtgaag
     3721 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     3781 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     3841 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     3901 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     3961 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     4021 ggcggcatgg acgagctgta caagtgagaa ttcgcggccg ctcaacctct ggattacaaa
     4081 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4141 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4201 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4261 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4321 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4381 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4441 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4501 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4561 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4621 cggatctccc tttgggccgc ctccccgcag gtacctttaa gaccaatgac ttacaaggca
     4681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4741 caaagaagtc aagatctgct ttttgcctgt actgggtctc tctggttaga ccagagtctc
     4801 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4861 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4921 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtttaa
     4981 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5041 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5101 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5161 gacagcaagg gggaggattg ggaagtcaat agcaggcatg ctggggatgc ggtgggctct
     5221 atgggcggcc gttaattaag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     5281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5341 atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7441 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     7501 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     7561 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     7621 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     7681 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     7741 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     7801 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     7861 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     7921 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     7981 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     8041 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg actagttatt
     8101 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     8161 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     8221 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     8281 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     8341 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     8401 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     8461 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     8521 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     8581 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8641 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     8701 gcctgggagc tctctggcta actagggaac ccact
//
