LOCUS       sequence-311601-        8638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 8638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1068..1301
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1486..1530
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1828..1945
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2020..2268
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2020..2040
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2191..2210
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2397..2884
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2432..2455)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2817..2836
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2924..3520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2924..2943)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3420..3440
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3539..3592
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3593..4294
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4354..4374)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4772..4783)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5235..5252)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5241..5465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5491..5511)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5491..5509)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5530..5546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5530..5546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5543..5565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5554..5570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5578..5584,5585..5602,5603..5608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5623..5644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5761..5778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5932..6520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6012..6031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6691..7482,7483..7551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7314..7333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7552..7656)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7682..8137)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7819..7840)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8031..8050
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8264..8286
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8278..8295
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8279..8295
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8305..8324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8305..8323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cggccgcgct gatcttcaga cctggaggag gagatatgag ggacaattgg
      961 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1021 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1081 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1141 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1201 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1261 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1321 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1381 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1441 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1501 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1561 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1621 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1681 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     1741 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     1801 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     1861 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     1921 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     1981 gttagtaccg ggccctacgc gtccaaggtc gggcaggaag agggcctatt tcccatgatt
     2041 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
     2101 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     2161 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     2221 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg ctacctgcat
     2281 aattttcagt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     2341 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tggatccaat tctaccgggt
     2401 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     2461 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     2521 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     2581 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     2641 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     2701 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     2761 gtccgggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     2821 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     2881 ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc
     2941 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3001 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     3061 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     3121 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     3181 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     3241 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     3301 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     3361 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     3421 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     3481 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc ggcggcgggt ccggaggaga
     3541 gggcagagga agtctcctaa catgcggtga cgtggaggag aatcctggcc caatgagcga
     3601 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
     3661 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     3721 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
     3781 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
     3841 cttccctgag ggcttcacat gggagagagt caccacatac gaggacgggg gcgtgctgac
     3901 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     3961 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
     4021 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
     4081 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
     4141 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
     4201 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
     4261 cctccctagc aaactggggc acaagcttaa ttgagcggcc gctcaacctc tggattacaa
     4321 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4381 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4441 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4501 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4561 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4621 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4681 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4741 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4801 ccgcggcctg ctgccggctc tgcggcctct tccgcgtcta cgccttcgcc ctcagacgag
     4861 tcggatctcc ctttgggccg cctccccgca ggtaccttta agaccaatga cttacaaggc
     4921 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     4981 ccaaagaagt caagatctgc tttttgcctg tactgggtct ctctggttag accagagtct
     5041 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5101 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5161 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagttta
     5221 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5281 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5341 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5401 ggacagcaag ggggaggatt gggaagtcaa tagcaggcat gctggggatg cggtgggctc
     5461 tatgggcggc cgttaattaa gcttttgttc cctttagtga gggttaattg cgcgcttggc
     5521 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5581 catacgagcc gggagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5641 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5701 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5761 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5821 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5881 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6121 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6361 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7561 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7621 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7681 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     7741 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     7801 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     7861 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     7921 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7981 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     8041 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     8101 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     8161 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     8221 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     8281 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gactagtaca
     8341 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     8401 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     8461 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     8521 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     8581 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgg
//
