LOCUS       sequence-311602-       10381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses ufd2-flag in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  TITLE     Ufd2 and Ufd4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJO818 (pRS425 Gal1-Ufd2-flag)
FEATURES             Location/Qualifiers
     source          1..10381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..80
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(574..1668)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1669..2076)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(2377..2832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2514..2535)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2726..2745
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2958..2980
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2972..2989
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2973..2989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2999..3018
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2999..3017
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(3036..3283)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3249..3267
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(3293..3316)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(6224..6665)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(6230..6253)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    6548..6665
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(6689..6709)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6689..6707)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6728..6744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6728..6744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6741..6763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6752..6768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6776..6782,6783..6800,6801..6806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6821..6842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6959..6976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7130..7718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7210..7229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7889..8680,8681..8749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8512..8531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8750..8854)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8881..10223
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9844..9891)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10264..10282
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10320..10342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatcga ctacgtcgtt aaggccgttt
      121 ctgacagagt aaaattcttg agggaacttt caccattatg ggaaatggtt caagaaggta
      181 ttgacttaaa ctccatcaaa tggtcaggtc attgagtgtt ttttatttgt tgtatttttt
      241 tttttttaga gaaaatcctc caatatataa attaggaatc atagtttcat gattttctgt
      301 tacacctaac tttttgtgtg gtgccctcct ccttgtcaat attaatgtta aagtgcaatt
      361 ctttttcctt atcacgttga gccattagta tcaatttgct tacctgtatt cctttacatc
      421 ctcctttttc tccttcttga taaatgtatg tagattgcgt atatagtttc gtctacccta
      481 tgaacatatt ccattttgta atttcgtgtc gtttctatta tgaatttcat ttataaagtt
      541 tatgtacaaa tatcataaaa aaagagaatc tttttaagca aggattttct taacttcttc
      601 ggcgacagca tcaccgactt cggtggtact gttggaacca cctaaatcac cagttctgat
      661 acctgcatcc aaaacctttt taactgcatc ttcaatggcc ttaccttctt caggcaagtt
      721 caatgacaat ttcaacatca ttgcagcaga caagatagtg gcgatagggt tgaccttatt
      781 ctttggcaaa tctggagcag aaccgtggca tggttcgtac aaaccaaatg cggtgttctt
      841 gtctggcaaa gaggccaagg acgcagatgg caacaaaccc aaggaacctg ggataacgga
      901 ggcttcatcg gagatgatat caccaaacat gttgctggtg attataatac catttaggtg
      961 ggttgggttc ttaactagga tcatggcggc agaatcaatc aattgatgtt gaaccttcaa
     1021 tgtagggaat tcgttcttga tggtttcctc cacagttttt ctccataatc ttgaagaggc
     1081 caaaacatta gctttatcca aggaccaaat aggcaatggt ggctcatgtt gtagggccat
     1141 gaaagcggcc attcttgtga ttctttgcac ttctggaacg gtgtattgtt cactatccca
     1201 agcgacacca tcaccatcgt cttcctttct cttaccaaag taaatacctc ccactaattc
     1261 tctgacaaca acgaagtcag tacctttagc aaattgtggc ttgattggag ataagtctaa
     1321 aagagagtcg gatgcaaagt tacatggtct taagttggcg tacaattgaa gttctttacg
     1381 gatttttagt aaaccttgtt caggtctaac actaccggta ccccatttag gaccacccac
     1441 agcacctaac aaaacggcat cagccttctt ggaggcttcc agcgcctcat ctggaagtgg
     1501 aacacctgta gcatcgatag cagcaccacc aattaaatga ttttcgaaat cgaacttgac
     1561 attggaacga acatcagaaa tagctttaag aaccttaatg gcttcggctg tgatttcttg
     1621 accaacgtgg tcacctggca aaacgacgat cttcttaggg gcagacatta gaatggtata
     1681 tccttgaaat atatatatat atattgctga aatgtaaaag gtaagaaaag ttagaaagta
     1741 agacgattgc taaccaccta ttggaaaaaa caataggtcc ttaaataata ttgtcaactt
     1801 caagtattgt gatgcaagca tttagtcatg aacgcttctc tattctatat gaaaagccgg
     1861 ttccggcgct ctcacctttc ctttttctcc caatttttca gttgaaaaag gtatatgcgt
     1921 caggcgacct ctgaaattaa caaaaaattt ccagtcatcg aatttgattc tgtgcgatag
     1981 cgcccctgtg tgttctcgtt atgttgagga aaaaaataat ggttgctaag agattcgaac
     2041 tcttgcatct tacgatacct gagtattccc acagttaact gcggtcaaga tatttcttga
     2101 atcaggcgcc ttagaccgct cggccaaaca accaattact tgttgagaaa tagagtataa
     2161 ttatcctata aatataacgt ttttgaacac acatgaacaa ggaagtacag gacaattgat
     2221 tttgaagaga atgtggattt tgatgtaatt gttgggattc catttttaat aaggcaataa
     2281 tattaggtat gtagatatac tagaagttct cctcgaccgg tcgatatgcg gtgtgaaata
     2341 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
     2401 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2461 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2521 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2581 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2641 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2701 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2761 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2821 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2881 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2941 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     3001 atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt cgagcgtccc
     3061 aaaaccttgc ttgagaaggt tttcagtata atgttacatg cgtacacgcg tctgtacaga
     3121 aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac tataaaaaaa
     3181 taaataggga cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg
     3241 gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgagt tacttgtcat
     3301 catcgtcctt gtagtcggat ccctcgcttg ctttatgttt tgcttcttcc tttttttgtt
     3361 ttttgaaaca aagaatcttt tgccttaact cttcattagg tgttacatcc tctagtttta
     3421 aaggcattct attaaacggg tcagtagagt cactaagcag atgtgctttg atcgtgcttc
     3481 tatcaatatt catcttggat gctggtaaaa tgacaggatc tttcataatt gtgtacatca
     3541 aaggatctaa gaactcgtca ggaacatcac catactcaag atcttcctct tcatcagcct
     3601 ttctttgctc ttcggcttta ttggcaaagt tcaatagttt ttcgatgaac tctggagatg
     3661 ctaaaccagt ttttctacca agaatatcaa ctgcgcgaac aaagagattt ctattaaatg
     3721 atctttcatc tttagcgaca gcactgatga actctgattg ctcggaaaga ttgatataaa
     3781 cagttgttaa ggctttcaac aaatcttttg ggttgaatga ataactttgt gggtctttca
     3841 cttttagttc cccgcatttg ggaccgacaa gtgactctaa gttatagttt agcatacttg
     3901 ccagtctata tacgatttct ggtgtgacaa atgcagccgg tatatccttc gaatagattt
     3961 cgaacagttt catcgattta tcggccaatc cacacgagga cttagcttgc ctggaagcag
     4021 acgccaatct tgtttgtagt tctttgtcct cttcctctct tgttggtggc gcaccccttg
     4081 cacgattatc cagctcattt tggatattat gcacctcagc caaattactt aggccttcat
     4141 ccaacaggaa agtcaagtca ttcaacatac gagcaacgaa cctcacaaaa aagtcagcat
     4201 tgttttgaga ctgccagatc aattggtttt tataagatgg tatcttgtaa taaagctcct
     4261 ccaaaattat tgatatgcta tatctgctgt tgaatttatc gtaaaattgt gaagaagagc
     4321 ctgttttttc aacaataaca taaaagtcta gtagagcata taacagattc ttgttaacta
     4381 attcgtcgtg ttcgaatata tccatcatga atccggggga attgtctgtt agaggcatag
     4441 caccaacgct taataactgc actaatttac cttttaaatg tgggttggaa accaattcag
     4501 gacaacggag gaccattgtt gtaaactcaa caaacgatcc cagacgtgga tttctgaaga
     4561 ttggagaggt ctggtacttc gaaatgtata gagaatagtt aactggacct tctacaacaa
     4621 attctggata gtatttaaaa ggcactggag catgcgctct caaaaaatca gcgttgtcca
     4681 cgttttcaac acctatttgg tccggaatta atggcaattt aatttgttta aacggaaatt
     4741 cgtgttcagg atcaacaaca cgaatcaaga acgtagatgc accacaaata aagtcgaaaa
     4801 cctctagttg aagagatcta tgagcgaaga acccttgaag agcaaatcgc aaagattctg
     4861 ttgttttcaa cgccttctcc atcttggata attgtgctgt aatgaatctg gcaaagacat
     4921 cgtggttcgc agctattttc tttacttttt caatttcttc ctttaacgct ttgatttcag
     4981 atcccatctt ctcttcaaat gataatgtac ctcccagacc ataatgcaaa taggttaatg
     5041 tcaagaaaaa gcaatcagag atgaagtttg gcttagaatc tgctgttttt ctgttcttgt
     5101 cataaaatgc atctgcctct ttaaaatcag aatttaatcg tgtttcccct gataaatcaa
     5161 taaataaact tgggttgttg aagtaatttg cgtcgatctt gtcgattttc ttatatgaaa
     5221 tatctagaaa tggttgagaa aatctaacca ataacaaagt gatattggac ataaatccgt
     5281 tagaggatag ttctttgaat ggtggatgat cggctctacg taaatggttt ttattagcga
     5341 tatgggcaaa ataactaatc atatcagtcc ttgagtttaa ggagccacga acaagcttat
     5401 cgacaataaa aaagagtcta tctatgacaa ctttatgctc agcttgtaga gattcatgga
     5461 tcatggctgt ctgttgtttg gaacgcaaaa ggttatcgcc gtagttccgg attgccacag
     5521 cagcttctat gggagaaaga gataaaatag gccccaatat cgttttcctt tcaaaatcct
     5581 gtggtttaca gctatagtct gcaaaaaaac catctatttt agtgaatatt tctgcaatag
     5641 gtttaaatgt aacaaataac tcaaaaatgg taagaacgtt attgtaaatt acagattcgt
     5701 tcaaatcaaa atggctgaca tgtttattac agtattctaa taatgtcggg aaaacagcgt
     5761 taagtaaatc caaagcggta ccttctaata tggctctttg aatgatttgg gaaaggaaat
     5821 cagtatatga gttaacgtta gaaacaattc cagtgatata attgatgaaa gcaccattca
     5881 tgcaaaagtt ttctatttgt aaggcaacga caccatagcc aatgacaaga cgatcaatct
     5941 cttggaaagt ggaatgtaat gactcagcat ttggcttgtt tttggtgatt cttttttgtt
     6001 gctgattacg gcgaaagcaa tcgttcaaat attcaaacgg tttatccaac ttctcattct
     6061 cggtgagttg atataatagt agcgtatcaa taaaatcaac tccaagagtt gagccttgag
     6121 gcacttcctc ggacttcaat agagagtagc cacgggtatc ggaaggatcg gtggtgattt
     6181 gtaaaatatc ttctatggcg gtcatggcgc gccggggttt tttctccttg acgttaaagt
     6241 atagaggtat attaacaatt ttttgttgat acttttatta catttgaata agaagtaata
     6301 caaaccgaaa atgttgaaag tattagttaa agtggttatg cagtttttgc atttatatat
     6361 ctgttaatag atcaaaaatc atcgcttcgc tgattaatta ccccagaaat aaggctaaaa
     6421 aactaatcgc attatcatcc tatggttgtt aatttgattc gttcatttga aggtttgtgg
     6481 ggccaggtta ctgccaattt ttcctcttca taaccataaa agctagtatt gtagaatctt
     6541 tattgttcgg agcagtgcgg cgcgaggcac atctgcgttt caggaacgcg accggtgaag
     6601 acgaggacgc acggaggaga gtcttccttc ggagggctgt cacccgctcg gcggcttcta
     6661 atccgtacta gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     6721 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6901 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7801 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7861 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8821 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8881 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     8941 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     9001 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
     9061 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     9121 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     9181 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     9241 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     9301 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     9361 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     9421 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     9481 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     9541 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     9601 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     9661 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     9721 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
     9781 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
     9841 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
     9901 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
     9961 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
    10021 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
    10081 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
    10141 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
    10201 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat
    10261 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    10321 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10381 a
//
