LOCUS       sequence-311606-        2631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas12a crRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA
  TITLE     Toward a translationally independent RNA-based synthetic oscillator 
            using deactivated CRISPR-Cas.
  JOURNAL   Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 
            10.1093/nar/gkaa557.
  PUBMED    32609820
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-08-20- 20"; volume: "48"; issue: 
            "14"; pages: "8165-8177"
COMMENT     Sequence Label: pJK504
FEATURES             Location/Qualifiers
     source          1..2631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..83
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(302..406)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     455..473
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(477..520)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        994..1028
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     primer_bind     complement(1145..1162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1279..1824)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1359..1378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2072..2631,1..232,233..301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
       61 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
      121 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
      181 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
      241 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      301 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      361 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      421 aagtgccacc tgacgtctaa gaaaccattt aattaatagg cgtatcacga ggcagaattt
      481 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcta aagatctcag
      541 aaatcatttt acaccaacgg gtcacacggg tattatgcta gctaatttct actgttgtag
      601 atcacctagc tcagtcctag gtctcggtac caaattccag aaaagaggcc tcccgaaagg
      661 ggggcctttt ttcgttttgg tccagactgt cgccggatgt gtatccgacc tgacgatggc
      721 ccaaaagggc cgaaacagtc ctctacaaat aattttgttt aagcttttta cacctccaca
      781 actagctggt attatgctag ctaatttcta ctgttgtaga tcaccaacgg gtcacacggg
      841 tctcggtacc aaattccaga aaagagacgc tttcgagcgt cttttttcgt tttggtccgg
      901 atccagtcat aagtctgggc taagcccact gatgagtcgc tgaaatgcga cgaaacttat
      961 gacctctaca aataattttg tttaagatag gattttacac ctagctcagt cctaggtatt
     1021 atgctagcta atttctactg ttgtagatca cctccacaac tagctggtct cggtaccaaa
     1081 gacgaacaat aagacgctga aaagcgtctt ttttcgtttt ggtccttaat taactgcagg
     1141 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     1201 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     1261 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     1321 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1381 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     1441 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     1501 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     1561 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     1621 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     1681 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     1741 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     1801 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     1861 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     1921 ttgttactag tgcttggcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca a
//
