LOCUS       sequence-311608-        9847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for mammalian overexpression of the mutated ER isoform of rat USP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Lu Y, Adegoke OA, Nepveu A, Nakayama KI, Bedard N, Cheng D, Peng J, 
            Wing SS
  TITLE     USP19 deubiquitinating enzyme supports cell proliferation by 
            stabilizing KPC1, a ubiquitin ligase for p27Kip1.
  JOURNAL   Mol Cell Biol. 2009 Jan;29(2):547-58. doi: 10.1128/MCB.00329-08. 
            Epub 2008 Nov 17.
  PUBMED    19015242
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00329-08"; journalName: "Mol Cell Biol"; date: 
            "2009-01"; volume: "29"; issue: "2"; pages: "547-58"
COMMENT     Sequence Label: pcDNA-HisFlag-rUSP19ER-CAmut
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        137..516
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        517..720
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     671..691
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     765..784
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        765..783
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      890..899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             902..919
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             920..985
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5338..5355)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5344..5568
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5614..6042
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5701..5720)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5911..5932
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6051..6071)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6056..6385
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6236..6371
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6452..7246
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6506..6525)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7116..7135
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7422..7543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7459..7478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7513..7532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7592..7608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7592..7608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7605..7627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7616..7632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7640..7646,7647..7664,7665..7670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7685..7706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7823..7840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7994..8582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8074..8093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8753..9544,9545..9613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9376..9395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9614..9718)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9793..9812)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
       61 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      121 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      181 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      241 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      301 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      361 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      421 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      481 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      541 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      601 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      661 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      721 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
      781 agggagaccc aagctggcta gttaagcttg gtaccgagct cggatccact agtccagtgt
      841 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ccaccatggc
      901 acatcatcat catcatcacg actacaaaga ccatgacggt gattataaag atcatgacat
      961 cgattacaag gatgacgatg acaagcgccc gggctgcagg aattcgatgg gtccatgtgc
     1021 tggcaccagt gctacaggcc caaggagggg gcctccggga ttggaggagg caactagtaa
     1081 gaagaaacag aaggatcgag caaaccagga aagtaaggat ggagatccta ggagagtgtc
     1141 catgcctcga aaggagccca ccaaagatga actgttgctc gattggagac agagttcaga
     1201 taaggtggtt gttaagctgc gcgtgggaac aggtcccata tgtctggagg aagtagatgc
     1261 tgctttcaca gacacggact gtgtggtgag gcttccagat ggtcggcagt ggggtggtgt
     1321 cttctttgct gaaatacaaa gttcttgtac caaagtgcag actcacaagg gtggtcttct
     1381 acagttggca ctaccaaaga aggtgcctct tctcacgtgg ccctctctcc tgaagaaacc
     1441 tctagggacc caagaactgg tgccaggatt gcggtgccag gagaacggac aagaactgtc
     1501 tcccattgcc ctggagccag gttctgagcc ccgcagagct aaacaggaag ctcgaaacca
     1561 gaagagggcc cagggccgtg gtgaggtagg ctcaggggct agccctgggg cacaggcagg
     1621 gcccagtgcc aagagggctg ttcatctctg cagagggcca gaaggtgaag ggtccatgga
     1681 tgggcctggc ccccagggtg atgccccatc cttcctgtcc gactcagcta cgcaggttga
     1741 ggctgaggag cagctccatg ttccaccagt gaaccctcaa acaagtctct tgggctcaga
     1801 gaagaattta gcccttttga cagtagagaa gacagtgtcc cccaggagtg actcagtctc
     1861 cccagtcatg atccggaaca gagaccctga gaaagatgac catttcaaag aggagatggc
     1921 agttggagct gatcctgcag ccttagcgga tgaaccagag tctatggtga acctggcatt
     1981 tgtcaagaat gattcgtatg agaaggggcc ggattcagtg gtggtgcacg tgtatgtgaa
     2041 ggagagccgc agggatacct ctcgagtact tttccgagag caggacttca cgctgatctt
     2101 ccagaccagg gatggaaatt ttctgaggct gcatccgggc tgtgggcccc acaccatctt
     2161 ccgctggcag gtgaagctca ggaacttgat tgagccagag caatgtacgt tttgtttcac
     2221 ggcctctcga atcgatatct gcctccggaa gcggcagagt cagcgctggg ggggactgga
     2281 ggcccctgct acacgagtgg gtggtgcaaa ggttgccgtg ccgacaggtc caaccccttt
     2341 ggattcaacc cctccaggag gtggccccct ccctctgact ggccaggagg aagccagggc
     2401 tgtagagaag gaaaaaccca aggctcggtc agaggactcg gggctggatg gtgtggtggc
     2461 tcgcaccccc ttggagcatg ttaccccaaa gccagaaccg cacttggcct cgcccaaacc
     2521 cacatgtatg gtgcctccaa tgcctcatag tccagtgagt ggagatagtg tggaggagga
     2581 tgaagaggaa gagaagaagg tttgtctccc aggctttact ggcctcgtca acttagggaa
     2641 caccgccttc atgaatagtg tcattcagtc tctgtccaac actcgggagc ttcgtgactt
     2701 cttccacgac cgatcctttg aggctgagat taactacaat aacccattgg ggactggtgg
     2761 gcgtctggct attggcttcg ctgtgctgct ccgggccctg tggaagggca ctcaccaagc
     2821 ctttcaaccc tccaagctaa aggccattgt agcaagcaag gccagccagt ttacaggcta
     2881 cgcacagcat gatgcccaag agttcatggc tttcttgttg gatgggctgc atgaagacct
     2941 gaatcggatc caaaacaaac cctacacaga gactgtggac tcagatgggc gacctgacga
     3001 ggtggtggct gaagaagcat ggcagcggca caagatgagg aacgactcat tcattgtgga
     3061 cctgtttcag ggccagtaca agtcaaagct ggtgtgccct gtgtgtgcca aggtctccat
     3121 cacttttgac ccgttccttt atctgccggt acccttgcca caaaagcaaa aggttctccc
     3181 catatattat tttgccagag agccccatag caaacccatc aagttcctgg tgagcgtcag
     3241 caaggagaac tccagcgcaa gcgaagtttt ggagtccctc tctcagagtg tccacgtgaa
     3301 gcctgagagc ctgcgcctgg ctgaggtaat taagaatcgt ttccaccgtg ttttcttgcc
     3361 ctcccactca ctggatgctg tgtcgccaac ggacgtgctc ctgtgctttg agctgctatc
     3421 cccagaattg gctaaggaga gggtagtggt gctagaggtg cagcagcgcc cccaggtacc
     3481 cagcatccct atctccaagt gtgcagcctg ccagcggaag cagcaatcag aagatgaaaa
     3541 actgaagcgt tgtacccgtt gctaccgtgt gggctactgc aaccagttct gtcagaaaac
     3601 ccattggcct gaccacaaag gcctctgccg ccccgagaac attggctacc ccttcctggt
     3661 cagtgtacct gcttcacgcc tcacttatgc ccgtcttgct cagctactag aaggttatgc
     3721 ccggtactct gtgagcgtat tccaaccacc cttccagcct ggccgaatgg ctttggaatc
     3781 acagagccct ggctgtacca cgttgctttc aaccagctct ctggaggctg gggatagtga
     3841 aagagaaccc attcagcctt ctgagctcca gttggtgact cctgtggctg agggggatac
     3901 cggggctcac cgcatgtggc cacctgctga tcggggtcct gtgcctagca ccagtggaat
     3961 ttcttccgag atgctggcca gtgggcctat ggaagggtgt tccttgcttg ctggtgagag
     4021 ggtgtctcgg cctgaagctg ctgtgcctgg gtaccagcac tcacgtgagt ctgtgagcgc
     4081 ccacacgccc cagttcttca tctataaaat tgatgcgtca agccgtgagc agcggctgga
     4141 ggacaaaggg gacactccac tggagctagg tgatgactgt agcctggctc tggtgtggcg
     4201 gaacaacgaa cgccttcagg agtttgtgct ggtggcctcc aaggagctgg agtgtgctga
     4261 agacccaggc tccgctggtg aggctgcccg tgctggccac ttcaccctgg accagtgcct
     4321 caatctcttt acacggcctg aagtgctggc acctgaggaa gcctggtact gcccacagtg
     4381 caaacagcat cgagaggcct ccaagcagct gctgctgtgg cgcctaccaa atgtgctcat
     4441 cgtgcagctc aagcgtttct cctttcgtag cttcatttgg cgtgacaaga tcaatgactt
     4501 ggtggagttt ccagttcgga acctggactt gagcaagttc tgtattggtc agaaagagga
     4561 gcagctgcct agctatgacc tgtatgcagt catcaaccac tacggaggca tgatcggcgg
     4621 ccactacact gcctgtgcac ggctgcccag tgaccgtagt agccagcgca gtgacgtggg
     4681 ctggcgcttg tttgatgaca gcacggtgac aacagtagac gagagccagg tggtgacacg
     4741 ctatgcctat gtgctcttct accgtcggcg gaactctcct gtggagaggc cccccagggc
     4801 agctcacgct gaacaccacc cagacctagg ccctgcagcc gaggctgctg ccagccaggc
     4861 ttcccggatt tggcaggagc tcgaggccga ggaggagatg gtgcccgagg ggcctgggcc
     4921 tctgggtcct tgggggcccc aagactgggt ggggcccccg ccacgtggcc ctaccacatc
     4981 agacgagggc tgcctccgat actttgtcct gggtaccgtg gcggctttgg tggcccttgt
     5041 gctcaacgta ttctatcctc tggtatctca gagtcgctgg agatgagctc ccctgcaggc
     5101 gcctgctgtg agctggccta cctatgctca ccaggacagg cctgcctttg tggtgggaga
     5161 cagctctggg ctctgagcct cagtgtatgt atctggtggg agacttgttg ggactacagc
     5221 ctctgtgggg gcagagtatc ctagggtgtg tgtccatcca gttgtctgtc ctcctgtttc
     5281 tctgaccctt gtcctagggc ttggggaatt cgatatcaag ctaaacccgc tgatcagcct
     5341 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5401 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5461 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5521 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5581 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5641 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5701 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5761 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5821 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5881 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5941 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6001 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     6061 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6121 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6181 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6241 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6301 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6361 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6421 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     6481 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6541 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6601 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     6661 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6721 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6781 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6841 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6901 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6961 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     7021 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     7081 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     7141 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7201 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     7261 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7321 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7381 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     7441 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7501 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7561 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7621 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7681 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7741 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7801 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7861 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8701 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8761 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8821 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8881 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8941 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9001 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9061 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9121 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9181 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9241 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9301 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9361 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9421 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9481 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9541 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9601 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9661 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9721 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9781 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9841 ttgtgtg
//
