LOCUS       sequence-31161-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Losev E, Reinke CA, Jellen J, Strongin DE, Bevis BJ, Glick BS
  TITLE     Golgi maturation visualized in living yeast.
  JOURNAL   Nature. 2006 Jun 22. 441(7096):1002-6.
  PUBMED    16699524
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jun 22. 441(7096):1002-6."
COMMENT     Sequence Label: YIPlac204-TC-Sec7-6xDsRed-M1
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      459..468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(645..665)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 cgtacgaatt ttctcgtgac tttaatgaag attatgggtt aagaacaaga ctagtagagg
       61 ctcgtgtagt tgataaaata ccaaacctgt tgaaacaaga aacgagtgct gctgcagttc
      121 tccttgacat tatgtttcag ttgtatctga acgatgatga gaagaaggct gatttaataa
      181 ctcgtctgat caccatttgc atccaggtcg tagagggtta tgtttctttg gatgatagaa
      241 ccatggaacg tagtatcaat gcctggcgtt cagtgatagt tgaaatatta caaggttact
      301 acgaatttga tgatgaagac tttagactat actgtcctgc tatgtatgct ctggtgattc
      361 aaatattaga taagagcgtt ccaacggagt tgagacatgc tataaaacaa tttctaagca
      421 gagttggtga attatacctt tctacggatc cactagtcgc caccatggac aacaccgagg
      481 acgtcatcaa ggagttcatg cagttcaagg tgcgcatgga gggctccgtg aacggccact
      541 acttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag accgccaagc
      601 tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
      661 agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac atgaagctgt
      721 ccttccccga gggcttcacc tgggagcgct ccatgaactt cgaggacggc ggcgtggtgg
      781 aggtgcagca ggactcctcc ctgcaggacg gcaccttcat ctacaaggtg aagttcaagg
      841 gcgtgactcc c
//
