LOCUS       sequence-31162-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Losev E, Reinke CA, Jellen J, Strongin DE, Bevis BJ, Glick BS
  TITLE     Golgi maturation visualized in living yeast.
  JOURNAL   Nature. 2006 Jun 22. 441(7096):1002-6.
  PUBMED    16699524
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jun 22. 441(7096):1002-6."
COMMENT     Sequence Label: YIPlac204-TC-Sec7-6xDsRed-M1
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(44..291)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     257..275
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(572..591)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aatttcacac aggaacagct atgaccatga ttacgccaag cttgcaaatt aaagccttcg
       61 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
      121 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
      181 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
      241 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atgactctag
      301 agtcgcggcc gctactggga gccggagtgg cgggcctcgg cgtgctcgta ctgctccacc
      361 acggtgtagt cctcgttgtg gttggtgatg tccagcttgg agtccacgta gtggttgccg
      421 ggcagctgca cgggcttctt ggccttgtac acggtcttga agtcgcaggt gtagtggccg
      481 ccgtccttca gcttcagggc gtgggagatc tcgcccttca gcacgccgtc ctgggggtac
      541 agcttctcgg tggagggctc ccagccggca gtcttcttct gcattacggg gccgtcggcg
      601 gggaagttca cgcccttgaa cttcaccttg tagatgaagg tgccgtcctg cagggaggag
      661 tcctgctgca cctccaccac gccgccgtcc tcgaagttca tggagcgctc ccaggtgaag
      721 ccctcgggga aggacagctt catgtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      781 ttggagccgt actggaactg gggggacagg atgtcccagc gaagggcagg gggccgccct
      841 tggtcacctg cagcttggcg gtctgggtgc cctcgtaggg cttg
//
