LOCUS       sequence-311623-        3591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Methanol oxidase (MOX) promoter module for episomal P. pastoris 
            promoter shuffling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Pullmann P, Weissenborn MJ
  TITLE     Improving the Heterologous Production of Fungal Peroxygenases 
            through an Episomal Pichia pastoris Promoter and Signal Peptide 
            Shuffling System.
  JOURNAL   ACS Synth Biol. 2021 Jun 2. doi: 10.1021/acssynbio.0c00641.
  PUBMED    34075757
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Jun 2. doi: 10.1021/acssynbio.0c00641."
COMMENT     Sequence Label: pAGM9121_pMOX
FEATURES             Location/Qualifiers
     source          1..3591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             285..1076
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1417..2005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1906..1925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgccgaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
       61 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
      121 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
      181 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      241 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
      301 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
      361 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      421 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
      481 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
      541 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
      601 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
      661 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
      721 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
      781 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
      841 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
      901 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
      961 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1021 tggaagaatt tgtccattac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     1081 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     1141 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     1201 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgctgt cagaccaagt
     1261 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1321 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1381 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1441 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1501 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1561 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1621 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1681 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1741 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1801 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1861 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1921 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1981 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctgca
     2041 ctctgtggtc tcaaacgcga cgcggagaac gatctcctcg agctgctcgc ggatcagctt
     2101 gtggcccggt aatggaacca ggccgacggc acgctccttg cggaccacgg tggctggcga
     2161 gcccagtttg tgaacgaggt cgtttagaac gtcctgcgca aagtccagtg tcagatgaat
     2221 gtcctcctcg gaccaattca gcatgttctc gagcagccat ctgtctttgg agtagaagcg
     2281 taatctctgc tcctcgttac tgtaccggaa gaggtagttt gcctcgccgc ccataatgaa
     2341 caggttctct ttctggtggc ctgtgagcag cggggacgtc tggacggcgt cgatgaggcc
     2401 cttgaggcgc tcgtagtact tgttcgcgtc gctgtagccg gccgcggtga cgatacccac
     2461 atagaggtcc ttggccatta gtttgatgag gtggggcagg atgggcgact cggcatcgaa
     2521 atttttgccg tcgtcgtaca gtgtgatgtc accatcgaat gtaatgagct gcagcttgcg
     2581 atctcggatg gttttggaat ggaagaaccg cgacatctcc aacagctggg ccgtgttgag
     2641 aatgagccgg acgtcgttga acgagggggc cacaagccgg cgtttgctga tggcgcggcg
     2701 ctcgtcctcg atgtagaagg ccttttccag aggcagtctc gtgaagaagc tgccaacgct
     2761 cggaaccagc tgcacgagcc gagacaattc gggggtgccg gctttggtca tttcaatgtt
     2821 gtcgtcgatg aggagttcga ggtcgtggaa gatttccgcg tagcggcgtt ttgcctcaga
     2881 gtttaccatg aggtcgtcca ctgcagagat gccgttgctc ttcaccgcgt acaggacgaa
     2941 cggcgtggcc agcaggccct tgatccattc tatgaggcca tctcgacggt gttccttgag
     3001 tgcgtactcc actctgtagc gactggacat ctcgagactg ggcttgctgt gctggatgca
     3061 ccaattaatt gttgccgcat gcatccttgc accgcaagtt tttaaaaccc actcgcttta
     3121 gccgtcgcgt aaaacttgtg aatctggcaa ctgagggggt tctgcagccg caaccgaact
     3181 tttcgcttcg aggacgcagc tggatggtgt catgtgaggc tctgtttgct ggcgtagcct
     3241 acaacgtgac cttgcctaac cggacggcgc tacccactgc tgtctgtgcc tgctaccaga
     3301 aaatcaccag agcagcagag ggccgatgtg gcaactggtg gggtgtcgga caggctgttt
     3361 ctccacagtg caaatgcggg tgaaccggcc agaaagtaaa ttcttatgct accgtgcagt
     3421 gactccgaca tccccagttt ttgccctact tgatcacaga tggggtcagc gctgccgcta
     3481 agtgtaccca accgtcccca cacggtccat ctataaatac tgctgccagt gcacggtggt
     3541 gacatcaatc taaagtacaa aaacaaatca tacaaaatgc gagaccacga a
//
