LOCUS       sequence-311634-       10727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of human Bcl11a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Bcl11a
FEATURES             Location/Qualifiers
     source          1..10727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    157..274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(331..349,350..372,373..391,392..414,415..433,434..456,
                     457..475,476..498,499..517,518..540,541..559,560..582,
                     583..601)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        634..672
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     669..693
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             896..907
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    3136..3724
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3189..3209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3607..3618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3727..3743)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3728..3744)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4249..4429
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4455..4472)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4461..4685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4731..5159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4818..4837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5028..5049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5168..5188)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5173..5502
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5353..5488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5550..5597
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5616..5990
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6120..6241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6157..6176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6211..6230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6290..6306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6290..6306)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6303..6325)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6314..6330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6338..6344,6345..6362,6363..6368))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6383..6404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6521..6538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6692..7280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6772..6791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7451..8242,8243..8311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8074..8093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8312..8416)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8491..8510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8682..9061
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9063..9261
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9279..9459
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9506..9631
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10124..10357
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10542..10586
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
       61 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
      121 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      181 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      241 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      301 tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      361 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
      421 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      481 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      541 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      601 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat
      661 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      721 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccggttct
      781 agagctagca tgtctcgccg caagcaaggc aaaccccagc acttaagcaa acgggaattc
      841 tcgcccgagc ctcttgaagc cattcttaca gatgatgaac cagaccacgg cccgttggga
      901 gctccagaag gggatcatga cctcctcacc tgtgggcagt gccagatgaa cttcccattg
      961 ggggacattc ttatttttat cgagcacaaa cggaaacaat gcaatggcag cctctgctta
     1021 gaaaaagctg tggataagcc accttcccct tcaccaatcg agatgaaaaa agcatccaat
     1081 cccgtggagg ttggcatcca ggtcacgcca gaggatgacg attgtttatc aacgtcatct
     1141 agaggaattt gccccaaaca ggaacacata gcagataaac ttctgcactg gaggggcctc
     1201 tcctcccctc gttctgcaca tggagctcta atccccacgc ctgggatgag tgcagaatat
     1261 gccccgcagg gtatttgtaa agatgagccc agcagctaca catgtacaac ttgcaaacag
     1321 ccattcacca gtgcatggtt tctcttgcaa cacgcacaga acactcatgg attaagaatc
     1381 tacttagaaa gcgaacacgg aagtcccctg accccgcggg ttggtatccc ttcaggacta
     1441 ggtgcagaat gtccttccca gccacctctc catgggattc atattgcaga caataacccc
     1501 tttaacctgc taagaatacc aggatcagta tcgagagagg cttccggcct ggcagaaggg
     1561 cgctttccac ccactccccc cctgtttagt ccaccaccga gacatcactt ggacccccac
     1621 cgcatagagc gcctgggggc ggaagagatg gccctggcca cccatcaccc gagtgccttt
     1681 gacagggtgc tgcggttgaa tccaatggct atggagcctc ccgccatgga tttctctagg
     1741 agacttagag agctggcagg gaacacgtct agcccaccgc tgtccccagg ccggcccagc
     1801 cctatgcaaa ggttactgca accattccag ccaggtagca agccgccctt cctggcgacg
     1861 ccccccctcc ctcctctgca atccgcccct cctccctccc agcccccggt caagtccaag
     1921 tcatgcgagt tctgcggcaa gacgttcaaa tttcagagca acctggtggt gcaccggcgc
     1981 agccacacgg gcgagaagcc ctacaagtgc aacctgtgcg accacgcgtg cacccaggcc
     2041 agcaagctga agcgccacat gaagacgcac atgcacaaat cgtcccccat gacggtcaag
     2101 tccgacgacg gtctctccac cgccagctcc ccggaacccg gcaccagcga cttggtgggc
     2161 agcgccagca gcgcgctcaa gtccgtggtg gccaagttca agagcgagaa cgaccccaac
     2221 ctgatcccgg agaacgggga cgaggaggaa gaggaggacg acgaggaaga ggaagaagag
     2281 gaggaagagg aggaggagga gctgacggag agcgagaggg tggactacgg cttcgggctg
     2341 agcctggagg cggcgcgcca ccacgagaac agctcgcggg gcgcggtcgt gggcgtgggc
     2401 gacgagagcc gcgccctgcc cgacgtcatg cagggcatgg tgctcagctc catgcagcac
     2461 ttcagcgagg ccttccacca ggtcctgggc gagaagcata agcgcggcca cctggccgag
     2521 gccgagggcc acagggacac ttgcgacgaa gactcggtgg ccggcgagtc ggaccgcata
     2581 gacgatggca ctgttaatgg ccgcggctgc tccccgggcg agtcggcctc ggggggcctg
     2641 tccaaaaagc tgctgctggg cagccccagc tcgctgagcc ccttctctaa gcgcatcaag
     2701 ctcgagaagg agttcgacct gcccccggcc gcgatgccca acacggagaa cgtgtactcg
     2761 cagtggctcg ccggctacgc ggcctccagg cagctcaaag atcccttcct tagcttcgga
     2821 gactccagac aatcgccttt tgcctcctcg tcggagcact cctcggagaa cgggagcttg
     2881 cgcttctcca caccgcccgg ggagctggac ggagggatct cggggcgcag cggcacggga
     2941 agtggaggga gcacgcccca tattagtggt ccgggcccgg gcaggcccag ctcaaaagag
     3001 ggcagacgca gcgacacttg ttcttcacac acccccattc ggcgtagtac ccagagagct
     3061 caagatgtgt ggcagttttc ggatggaagc tcgagagccc ttaagttctg attcgaagat
     3121 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3181 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3241 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3301 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3361 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3421 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3481 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3541 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3601 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3661 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3721 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     3781 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     3841 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     3901 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     3961 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     4021 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     4081 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     4141 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     4201 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     4261 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4321 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4381 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     4441 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4501 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4561 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4621 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4681 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4741 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4801 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4861 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4921 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4981 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5041 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5101 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5161 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5221 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5281 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5341 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5401 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5461 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5521 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     5581 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     5641 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     5701 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     5761 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     5821 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     5881 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     5941 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     6001 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6061 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6121 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6241 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6301 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6361 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6421 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6481 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6541 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6601 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7441 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7501 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7561 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7621 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7681 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7741 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7801 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7861 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7921 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7981 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8041 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8101 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8161 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8221 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8281 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8401 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8461 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8521 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     8581 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     8641 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     8701 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     8761 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     8821 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     8881 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     8941 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     9001 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     9061 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     9121 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     9181 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     9241 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     9301 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9361 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9421 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     9481 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     9541 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     9601 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     9661 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     9721 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     9781 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     9841 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     9901 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     9961 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    10021 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10081 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10141 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    10201 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    10261 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    10321 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    10381 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    10441 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    10501 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    10561 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    10621 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    10681 tactttctat agtgaataga gttaggcagg gatattcacc attatcg
//
