LOCUS       sequence-311642-       13996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of 
            DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13996)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 13996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH4531
FEATURES             Location/Qualifiers
     source          1..13996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..90
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(243..248,249..266,267..273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    281..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     286..308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     305..321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     305..321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11225..11242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11225..11241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11234..11256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11454..11909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11541..11560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11751..11772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11926..11945)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12045..12067
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12105..12123)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12191..12295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12296..12364,12365..13156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12514..12533)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13327..13915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13816..13835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
       61 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      121 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      301 cacacaggaa acagctatga catgattacg aattcgaatg gccatgggac gtcgacgctt
      361 gcatgcctgc agcgtttccg ataccccctt atatcagcac acattcagtc atcataccac
      421 aaaatgttgt gatagtgata ggcaaaacta ccgtatttcc tctattaatc ttgcatgcaa
      481 gactaatttt cgattaaccc gtaggggtgc aagactaata gagactgcaa gactattaga
      541 ggctgaaata ctaattttcg tatgctcaat aattttggaa attggcctat tttttgtaga
      601 aacttgatac cgtttaaaca aggaaaaata cacacttttt aatatttcat caataatttg
      661 aacgattttg tgattttaag ttcaatttgc ccaaaaaaag acaatttttc tgacgttacc
      721 gcaaagtaat gctggtcagg caaaaagagc ggtgcaaaaa tataagagac tgcaatacta
      781 atagaggaaa tacggtaatt gaattttagt gaaacttgcg acagttttcc tcattttttt
      841 gttattccgg catacgagtg tggcatacga gtgaggtcat cttttgtttc ttccgtttct
      901 tcatcgcttt tgaaaaaaaa tgttgaagaa aacttcgtag ccgatagccg gataatgttt
      961 ggcacggcgt tccaacatat atcattggaa attttttaag atttttgcga aaaatcacat
     1021 tcttcacgat gagaacacgt tattgaagga atataaatca agaataacat atagttatat
     1081 ttctctatta ctttaacgtt aaatatgagc aaatttgagc attttaattg cgatgaaaag
     1141 cagaatcgag tcaactgaaa tccgttcaaa aacctaagct cggttcgccc gaaagtcgat
     1201 ttttccaata gccgaacagc actgcttctt tattttccag gtatttttgg ggtgtaagaa
     1261 ttatatcttc tttgtttggg tatttacgtt tccgaattcc ctttcttgat ctgtcgctat
     1321 ttttcagaaa ccagaacagt ttatcctttt tcatgagaac ctgaatagct caaaaacccg
     1381 tttaatttct tttcatcatc tcatttgaaa ctttcctagt cttcttaatg atttccaggc
     1441 tcctcctaca tttgtatctc aagatgcata cactttattt ccgctccaat tcgaatcatc
     1501 tacttctctt ttttcttcaa ctttctactt ttttccattc ttttctttca aatgttcgac
     1561 gtcttcaagt gactgctctt cattctcctc atttttctcg aatttcattc ttgtctcttt
     1621 tttgctcaaa aatggaaaat gaaagtgacg tgagatttgg acggcgggac acgggggcag
     1681 tagaagcagc aaaaaggaga gaaagaggac acaaataaga agaacgaatt caaaaataag
     1741 cggagaggag ctatttccgt caattctacc tccccaatct tcatcaattc ggctcaattg
     1801 aatgacgtca cagggagata aaccgtttgg atgagcgccg tccatcactg acgccatccc
     1861 gtttgggaca agaaaagaga aaaaagagca caaagttttt ggtgacggat cttgtcaatc
     1921 atatgaaagt tgttctgatt gattgtcagt ttttttccta cttttttgga ttctatccac
     1981 ttctgaactt ttgacaagtt tcaaactttc tgaatcattt tctatgcatt ttcctggaat
     2041 tctttttatg taaaatatga aatagaatgt ttttgaattc aagttctgct tttttcttct
     2101 tttttgttct gttcgggctt gggtatgctt tttttaaaaa ttattttgca catcgaccaa
     2161 taagtgcgca acttataaaa ttaatttatt tttgttaatt tttgaaatac ttgtattgct
     2221 ttaagtgatc tgacctcgcc ctgagctttc cacgtagtta tcaaatacaa atcctccaag
     2281 ggtaacgtac ctatattact gatctttata ataactttat cacctgtcca gttccagagg
     2341 aattctgtta agcttatagg cacagaagga gtcatttctg ctggttttta atgatccaaa
     2401 tctttatttc aagtaaaaaa ctgaacactt gcgaataaaa ctatcagatt aaccattcac
     2461 caaaaatgtg tttgaatcta aaactttctc agtattccaa atatagaaat aaataaccac
     2521 gacattgcta aaatctgtct gaattgtgta ctccttaccg tgaagtaata atggatataa
     2581 tgaatcgttt gaaatgaatg atcagcacat ttttggtgaa agatcacaaa taaggaataa
     2641 gcgacggaaa ataaaacgat tatttcggat caaaaatttg ttgaagatca tatacactcg
     2701 agaccaagat tattctagac aattttcaaa ttggcttctt tgtttgcaaa tcttcataat
     2761 aatctgtgaa gtttggaaat ttgaattttt aatctttttt catagattat agtttttatt
     2821 tctttgcaaa actatattaa aaaccgatgc attgttttag ggaaattaat gagccttttg
     2881 ttcaacacta aaaacaataa aattaaaatt ttggcttcat catttgacct tttttaagtt
     2941 cgaaaacttt tctcgtattt ctgaaccgcc aattttttca cacatctcta gacttttggt
     3001 gcccgttcca gaaagttaag taattgctat tctaagaaag ttctcaacat tgttttttag
     3061 ttctgattga attctgatgt tccaggaata tattttaaat ttaatattct ttgcactatt
     3121 tctatactaa ctaaataata aatagtctaa gtatgttcaa tgagaacaaa aaagctctat
     3181 ctatattttt ctatcctatt tcatttatat ccttttcatt ttgaactcac cagcttctct
     3241 tcttcttctt gtccatatac ttcttaaaag tctcgaactt ctctctctca ctctccatca
     3301 atttctttat ggctaccgct tgcgctctgc cgcttccgaa gaaagaaggc gttggcagag
     3361 ctctcaattc tattttttct cgccgtaggc tatcgatttc tcagcttctc ttctctcttc
     3421 ctcactccac caccaccact tcgggcttct tcttttcttc attttcgtct tcttcttcat
     3481 cctcttcttt ttttttcagt tccccctcac tcctcccctt atatgcgcgt gcgagagggg
     3541 tgcaaaagca gcgcgcatcc gagaattgaa acgagaaaac ggagacgcag cagcagttcg
     3601 tcctcagaaa aatagccagt aaaaagagaa aaagatagag agagacctat cgcattttat
     3661 tttcaaattg caattcctgc aattcatgtg tgcgtgtgcc cattttcaat tcttcccgtt
     3721 atttttcaga ccattaccaa cgttctttct agatcttgag acaatcttcc ttctgctcaa
     3781 tcgttcgtcg tgaagacggc atcgacgacg acgacgatca gcagtaaacc cgggatgaat
     3841 attgtcagat gtcggagacg acacaaaatt ttggaaaatt tggaagaaga gaatctcggc
     3901 ccgagctgct cgtcgacgac ttcaacaacc gctgccaccg aagctctcgg aacaaccact
     3961 gaggatatga ggcttaagca gcagcgaagc tcgtcgcgtg ccacggagca cgatattgtc
     4021 gacggcaatc accacgacga cgagcacatc acaatgagac ggcttcgact tgtcaaaaat
     4081 tcgcggacgc ggcgtagaac gacgcccgat tcaagtatgg actgctatga ggaaaacccg
     4141 ccatcacaaa aaacttcaat aaattattct tggatttcta aaaagtcatc aatgacgtca
     4201 ttaatgcttt tactgctatt cgcttttgta cagccgtgtg cctcaatagt cgaaaaacga
     4261 tgcggcccaa tcgatattcg aaataggccg tgggatatta agccgcaatg gtcgaaactt
     4321 ggtgatccga acgaaaaaga tttggctggt cagagaatgg tcaactgcac agtggtggaa
     4381 ggttcgctga caatctcatt tgtactgaaa cacaagacaa aagcacaaga agaaatgcat
     4441 cgaagtctac agccaagata ttcccaagac gaatttatca cttttccgca tctacgtgaa
     4501 attactggaa ctctgctcgt ttttgagact gaaggattag tggatttgcg taaaattttc
     4561 ccaaatcttc gtgtaattgg aggccgttcg ctgattcaac actatgcgct gataatttat
     4621 cgaaatccgg atttggagat cggtcttgac aagctttccg taattcgaaa tggtggtgta
     4681 cggataatcg ataatcgaaa actgtgctac acgaaaacga ttgattggaa acatttgatc
     4741 acttcttcca tcaacgatgt tgtcgttgat aatgctgccg agtacgctgt cactgagact
     4801 ggattgatgt gcccacgtgg agcttgcgaa gaggataaag gcgaatcaaa gtgtcattat
     4861 ttggaggaaa agaatcagga acaaggtgtc gaaagagttc agagttgttg gtcgaacacc
     4921 acttgccaaa agtcttgtgc ttatgatcgt cttcttccaa cgaaagaaat cggaccggga
     4981 tgtgatgcga acggcgatcg atgtcacgat caatgcgtgg gcggttgtga gcgtgtgaat
     5041 gatgcgacag catgccacgc gtgcaagaat gtctatcaca agggaaagtg tatcgaaaag
     5101 tgtgatgctc acctgtacct tctccttcaa cgtcgttgtg tgacccgtga gcagtgtctg
     5161 cagctgaatc cggtgctctc gaacaaaaca gtgcctatca aggcgacggc aggcctttgc
     5221 tcggataaat gtcccgatgg ttatcaaatc aacccggatg atcatcgaga atgccgaaaa
     5281 tgcgttggca agtgtgagat tgtgtgcgag atcaatcacg tcattgatac gtttccgaag
     5341 gcacaggcga tcaggctatg caatattatt gacggaaatc tgacgatcga gattcgcgga
     5401 aaacaggatt cgggaatggc gtccgagttg aaggatatat ttgcgaacat tcacacgatc
     5461 accggctacc tgttggtacg tcaatcgtca ccgtttatct cgttgaacat gttccggaat
     5521 ttacgacgta ttgaggcaaa gtcactgttc agaaatctat atgctatcac agtttttgaa
     5581 aatccgaatt taaaaaagct attcgattca acgacggatt tgacgcttga tcgtggaact
     5641 gtgtcaattg ccaataacaa gatgttatgc ttcaagtata tcaagcagct aatgtcaaag
     5701 ttaaatatac cactcgatcc gatagatcaa tcagaaggga caaatggtga gaaggcaatc
     5761 tgtgaggata tggcaatcaa cgtgagcatc acagcggtca acgcggactc ggtcttcttt
     5821 agttggccct cattcaacat taccgatata gatcagcgaa agtttctcgg ctacgagctc
     5881 ttcttcaaag aagtcccacg aatcgatgag aacatgacga tcgaagagga tcgaagtgcg
     5941 tgtgtcgatt cgtggcagag tgtcttcaaa cagtactacg agacgtcgaa cggtgaaccg
     6001 accccggaca tttttatgga tattggaccg cgcgagcgaa ttcggccgaa tacgctctac
     6061 gcgtactatg tggcgacgca gatggtgttg catgccggtg cgaagaacgg tgtatcgaag
     6121 attggttttg tgaggacgag ctactatacg cctgatcctc cgacgttggc actagcgcaa
     6181 gtcgattcgg acgctattca tattacgtgg gaagcgccgc tccaaccgaa cggagacctc
     6241 acgcattaca caattatgtg gcgtgagaat gaagtgagcc cgtacgagga agccgaaaag
     6301 ttttgtacag atgcaagcac ccccgcaaat cgacaacaca cgaaagatcc gaaagagacg
     6361 attgtagccg ataagccagt cgatattccg tcatcacgta ccgtagctcc gacacttttg
     6421 actatgatgg gtcacgaaga tcagcagaaa acgtgcgctg caacgcccgg ttgttgttcg
     6481 tgttcggcta tcgaagaatc atcggaacag aacaagaaga agcgaccgga tccgatgtcg
     6541 gcgatcgaat catctgcatt tgagaataag ctgttggatg aggttttaat gccgagagac
     6601 acgatgcgag tgagacgatc aattgaagac gcgaatcgag tcagtgaaga gttggaaaaa
     6661 gctgaaaatt tgggaaaagc tccaaaaact ctcggtggaa agaagccgct gatccatatt
     6721 tcgaagaaga agccgtcgag cagcagcacc acatccacac cggctccaac gatcgcatca
     6781 atgtatgcct taacaaggaa accgactacg gtgccgggaa caaggattcg gctctacgag
     6841 atctacgaac ctttacccgg aagctgggcg attaatgtat cagctctggc attggataat
     6901 agttatgtga tacgaaattt gaagcattac acactttatg cgatttctct atccgcgtgc
     6961 caaaacatga cagtacccgg agcatcttgc tcaatatccc atcgtgcggg agcattgaaa
     7021 cgaacaaaac acatcacaga cattgataaa gtgttgaatg aaacaattga atggagattt
     7081 atgaataata gtcaacaagt caacgtgacg tgggatccac cgactgaagt gaatggtgga
     7141 atattcggtt atgttgtaaa gcttaagtca aaagtcgatg gatcaattgt tatgacgaga
     7201 tgtgtcggtg cgaagagagg atattcaaca cggaatcagg gtgtcctatt ccagaatttg
     7261 gccgatggac gttattttgt ctcagtaacg gcgacctctg tacacggcgc tggaccggaa
     7321 gccgaatcct ccgacccaat cgtcgtcatg acgccaggct tcttcactgt ggaaatcatt
     7381 ctcggcatgc ttctcgtctt tttgatttta atgtcaattg ccggttgtat aatctactac
     7441 tacattcaag tacgctacgg caaaaaagtg aaagctctat ctgactttat gcaattgaat
     7501 cccgaatatt gtgtggacaa taagtacaat gcagacgatt gggagctacg gcaggatgat
     7561 gttgtgctcg gacaacagtg tggagaggga tcattcggaa aagtgtacct cggaactgga
     7621 aataatgttg tttctctgat gggtgatcgt ttcggaccgt gtgctattaa gattaatgta
     7681 gatgatccag cgtcgactga gaatctcaac tatctcatgg aagctaatat tatgaagaac
     7741 tttaagacta actttatcgt caaactgtac ggagttatct ctactgtaca accagcgatg
     7801 gttgtgatgg aaatgatgga tcttggaaat ctccgtgact atctccgatc gaaacgcgaa
     7861 gacgaagtgt tcaatgagac ggactgcaac tttttcgaca taatcccgag ggataaattc
     7921 catgagtggg ccgcacagat ttgtgatggt atggcgtacc tggagtcgct caagttttgc
     7981 catcgagatc tcgccgcacg taattgcatg ataaatcggg atgagactgt caagattgga
     8041 gatttcggaa tggctcgtga tctattctat catgactatt ataagccatc gggcaagcgt
     8101 atgatgcctg ttcgatggat gtcacccgag tcgttgaaag acggaaagtt tgactcgaaa
     8161 tctgatgttt ggagcttcgg agttgttctc tatgaaatgg ttacactcgg tgctcagcca
     8221 tatattggtt tgagtaatga tgaggtgttg aattatattg gaatggcccg gaaggttatc
     8281 aagaagcccg aatgttgtga aaactattgg tataaggtga tgaaaatgtg ctggagatac
     8341 tcacctcggg atcgtccgac gttcctccag ctcgttcatc ttctagcagc tgaagcttca
     8401 ccagaattcc gagatttatc atttgtccta accgataatc aaatgatcct tgacgattca
     8461 gaagcactgg atcttgatga tattgatgat actgatatga atgatcaggt tgtcgaggtg
     8521 gcaccggatg ttgagaacgt cgaggttcag agtgattcgg aacgtcggaa tacggattca
     8581 ataccgttga aacagtttaa gacgatccct ccgatcaatg cgacgacgag tcattcgaca
     8641 atatcgattg atgagacacc gatgaaagcg aagcagcgag aaggatcgct ggatgaggag
     8701 tacgcattga tgaatcatag tggaggtccg agtgatgcgg aagttcggac gtatgctggt
     8761 gatggagatt atgtggagag agatgttcga gagaatgatg tgccaacgcg acgaaatact
     8821 ggtgcatcaa catcaagtta cacaggtggt ggtccatatt gcctaacaaa tcgtggtggt
     8881 tcaaatgaac gaggagccgg tttcggtgaa gcagtacgat taactgatgg tgttggaagt
     8941 ggacatttaa atgatgatga ttatgttgaa aaagagatat catccatgga tacgcgccgg
     9001 agcacgggcg cctcgagctc ttcctacggt gttccacaga cgaattggag tggaaatcgt
     9061 ggtgccacgt attatacgag taaagctcaa caggcagcaa ctgcagcagc agcagcagca
     9121 gcagctctcc aacagcaaca aaatggtggt cgaggcgatc gattaactca actacccgga
     9181 actggacatt tacaatcgac acgtggtgga caagatggag attatattga aactgaaccg
     9241 aaaaattata gaaataatgg atctccatcg gtactggatt tttttttttg gagaaattag
     9301 ccggaaaatt cgattttttt cgctgaaaat ctgaaaaaat ttggctaaaa attgccctga
     9361 aacacgtttt tttcggaatt ttcatataaa aatggacatt ttcgtaaaaa attggctatg
     9421 tgttgtgaat tttttctgac ttttaaaggc ctttgaacta ggaatattaa aattttattg
     9481 aaaattattg aaaagtgcaa atttttattt tttttaggtg aaaaaagctt agatttaggc
     9541 gaattttctg aaattttata gtgaatttgg ttctcaggat attagccatt ttttcccgat
     9601 tttttggtaa aaatttgtta aaacccgctt caaatgactg tagttttata caaaaataac
     9661 taaaaattcg atttttcaat tattcagctc gaaaaagccc aacaaaaata tttaaaattt
     9721 gaagaaaaaa gctaaaaatg agtaaaaatg actggttttt ttcgcttttt ctatttgaat
     9781 aaatttctga aaaatgacga aaatctgaaa aaattacccc ggacattggg aaatttcgaa
     9841 aaaatcttga aaatagtctg aaatttttaa aatttacaga ttttcacaaa aaagggaaat
     9901 ttcgcatttc ctgaaattgc ccaaaaattt tttttttcat ttttttaaaa attctggaaa
     9961 tttatttttg gctgtttcaa atttttaaaa aaagaaaaat cccaaaaatt ggtgaaaact
    10021 ccattaaaat tctccagaaa attcaaattt tccatgaaaa attgttttaa aagcatgaaa
    10081 atctgccgat ttttgtctaa aatttttaaa aatatttcaa aaatttcaga aaaaacagga
    10141 aaagcgaata ttacataaat aaaaaatctg gaaattctag aaactgaaaa ttttttaatt
    10201 tttccgctca aaaattctaa tattcgatga aaatgagccc gaaaattcga gtaaatttcc
    10261 aaaaaaaatt tttttttttt tcaaacacaa atattaattt ttattaattt ttcagcgaaa
    10321 cggcaacagc cgtgacattt tcaacggacg ttcggctttc ggtgaaaatg agcatctaat
    10381 cgaggataat gagcatccac ttgtggtacc atggtattga tatctgagct ccgcatcggc
    10441 cgctgtcatc agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt
    10501 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
    10561 aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
    10621 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
    10681 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
    10741 ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct
    10801 tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct
    10861 ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca
    10921 atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt
    10981 gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac
    11041 gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt
    11101 tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt
    11161 cccaatatac caaacataac tgtttctact agtggccacg tgggccgtgc accttaagct
    11221 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    11281 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    11341 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
    11401 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
    11461 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
    11521 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    11581 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
    11641 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
    11701 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    11761 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
    11821 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
    11881 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
    11941 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    12001 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
    12061 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
    12121 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    12181 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    12241 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    12301 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    12361 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    12421 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    12481 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    12541 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    12601 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    12661 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    12721 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    12781 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    12841 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    12901 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    12961 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    13021 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    13081 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    13141 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    13201 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    13261 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    13321 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    13381 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    13441 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    13501 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    13561 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    13621 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    13681 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    13741 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    13801 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    13861 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    13921 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    13981 tcctgcgtta tcccct
//
