LOCUS       sequence-311646-       11841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1464..1529
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1659..1679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2104..2238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2141..2160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2195..2214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2406..3039
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3086..3211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3708..3941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4126..4170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4455..4572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4725..5132
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5927..7420
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7517..7537
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7602..8190
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7655..7675)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8073..8084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8193..8209)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8194..8210)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8300..8533
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8558..8576)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8559..8576)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8866..8885)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9099..9118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9218..9240
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9278..9296)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9364..9468
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9469..9537,9538..10329)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9687..9706)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10500..11088
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10989..11008
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    11376..11397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11412..11417,11418..11435,11436..11442)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11450..11466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
      361 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
      421 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
      481 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
      541 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
      601 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
      661 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
      721 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
      781 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
      841 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
      901 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
      961 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     1021 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     1081 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     1141 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     1201 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     1261 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     1321 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     1381 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     1441 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     1501 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     1561 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     1621 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     1681 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     1741 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     1801 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     1861 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     1921 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     1981 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2041 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2101 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2221 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     2281 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     2341 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     2401 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     2461 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     2521 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     2581 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     2641 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     2701 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     2761 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     2821 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     2881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3061 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     3121 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     3181 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     3241 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     3301 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3361 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3421 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3481 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3541 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3601 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3661 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3721 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3781 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3841 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3901 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3961 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     4021 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     4081 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     4141 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     4201 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4261 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4321 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4381 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca
     4441 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4501 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4561 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt
     4621 gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
     4681 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca
     4741 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
     4801 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
     4861 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
     4921 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
     4981 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
     5041 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
     5101 cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca
     5161 cgcgtcaagt ggagcaaggc aggtggacag tggatccgga gctaccatgg tgtctaaagg
     5221 cgaggaggat aacatggcta taatcaagga atttatgagg ttcaaggtgc atatggaggg
     5281 atctgtgaac ggtcacgagt tcgaaatcga gggcgaaggc gaggggcgac cctatgaagg
     5341 gacacagacg gccaaactga aggtgaccaa gggtggcccc ctgcctttcg cctgggacat
     5401 cctgtcaccc cagtttatgt acggctctaa ggcttacgtt aagcaccctg ccgatattcc
     5461 ggactacttg aaactgagct tccctgaagg gttcaagtgg gaacgggtaa tgaattttga
     5521 ggatggcggt gtagtcacag ttacccagga cagctctctt caggacggag aatttatcta
     5581 taaggttaaa cttcggggca ctaacttccc atcagacggc cccgtgatgc agaagaaaac
     5641 tatgggctgg gaagccagct cagaacgcat gtatcccgag gacggagcac tgaagggaga
     5701 aatcaagcag cgactcaagc tgaaggatgg gggacattat gatgctgagg tcaaaaccac
     5761 ctataaggcc aagaaacccg ttcagcttcc tggggcatac aacgtgaata tcaaactgga
     5821 tattacatcc cataacgaag actataccat cgtggaacag tacgagcggg ccgagggcag
     5881 acatagcaca gggggcatgg atgaattgta caaggcggcc acgcgtatga agatggacaa
     5941 aaagactata gtttggttta gaagagacct aaggattgag gataatcctg cattagcagc
     6001 agctgctcac gaaggatctg tttttcctgt cttcatttgg tgtcctgaag aagaaggaca
     6061 gttttatcct ggaagagctt caagatggtg gatgaaacaa tcacttgctc acttatctca
     6121 atccttgaag gctcttggat ctgacctcac tttaatcaaa acccacaaca cgatttcagc
     6181 gatcttggat tgtatccgcg ttaccggtgc tacaaaagtc gtctttaacc acctctatga
     6241 tcctgtttcg ttagttcggg accataccgt aaaggagaag ctggtggaac gtgggatctc
     6301 tgtgcaaagc tacaatggag atctattgta tgaaccgtgg gagatatact gcgaaaaggg
     6361 caaacctttt acgagtttca attcttactg gaagaaatgc ttagatatgt cgattgaatc
     6421 cgttatgctt cctcctcctt ggcggttgat gccaataact gcagcggctg aagcgatttg
     6481 ggcgtgttcg attgaagaac tagggctgga gaatgaggcc gagaaaccga gcaatgcgtt
     6541 gttaactaga gcttggtctc caggatggag caatgctgat aagttactaa atgagttcat
     6601 cgagaagcag ttgatagatt atgcaaagaa cagcaagaaa gttgttggga attctacttc
     6661 actactttct ccgtatctcc atttcgggga aataagcgtc agacacgttt tccagtgtgc
     6721 ccggatgaaa caaattatat gggcaagaga taagaacagt gaaggagaag aaagtgcaga
     6781 tctttttctt aggggaatcg gtttaagaga gtattctcgg tatatatgtt tcaacttccc
     6841 gtttactcac gagcaatcgt tgttgagtca tcttcggttt ttcccttggg atgctgatgt
     6901 tgataagttc aaggcctgga gacaaggcag gaccggttat ccgttggtgg atgccggaat
     6961 gagagagctt tgggctaccg gatggatgca taacagaata agagtgattg tttcaagctt
     7021 tgctgtgaag tttcttctcc ttccatggaa atggggaatg aagtatttct gggatacact
     7081 tttggatgct gatttggaat gtgacatcct tggctggcag tatatctctg ggagtatccc
     7141 cgatggccac gagcttgatc gcttggacaa tcccgcgtta caaggcgcca aatatgaccc
     7201 agaaggtgag tacataaggc aatggcttcc cgagcttgcg agattgccaa ctgaatggat
     7261 ccatcatcca tgggacgctc ctttaaccgt actcaaagct tctggtgtgg aactcggaac
     7321 aaactatgcg aaacccattg tagacatcga cacagctcgt gagctactag ctaaagctat
     7381 ttcaagaacc cgtgaagcac agatcatgat cggagcagca gcccgggatc caccggtcgc
     7441 caccggttca gggagcggat ccggctcagg gagtgcggcc ggaactccca cctgcaacat
     7501 gcgtgacgga ggcggtccaa aaaagaagag aaaggtatga ctgaggccgc gactctagag
     7561 tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca
     7621 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7681 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7741 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7801 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7861 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7921 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7981 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     8041 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8101 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     8161 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat
     8221 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8281 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     8341 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8521 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
     8581 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
     8641 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
     8701 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
     8761 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
     8821 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     8881 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     8941 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     9001 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     9061 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
     9121 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9181 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9241 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     9301 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     9361 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9421 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9481 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9541 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9601 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9661 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9721 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9781 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9841 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9901 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9961 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    10021 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    10081 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    10141 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    10201 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10261 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10321 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10381 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10441 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10501 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10561 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10621 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10681 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10741 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10801 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10861 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10921 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10981 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    11041 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    11101 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    11161 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    11221 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    11281 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc
    11341 agttagggkg wrgmrggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    11401 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    11461 taacaatttc ttttattgag ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa
    11521 ctgattggtt agttcaaata aggcacaggg tcatttcagg tccttggggc accctggaaa
    11581 catctgatgg ttctctaggg tcgacccttt ttatatttta cgkttcctgg ccttttgctg
    11641 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    11701 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    11761 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    11821 tcattaatgc agctgtggaa t
//
