LOCUS       sequence-311648-       11720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1464..1529
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1659..1679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2104..2238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2141..2160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2195..2214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2406..3039
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3086..3211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3708..3941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4126..4170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4455..4572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4725..5132
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6314..7807
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7904..7924
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7989..8577
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8042..8062)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8460..8471)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8580..8596)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8581..8597)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8687..8920
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8945..8963)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8946..8963)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9253..9272)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9486..9505)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9605..9627
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9665..9683)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9751..9855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9856..9924,9925..10716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10074..10093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10887..11475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11376..11395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11629..11646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(join(11716..11720,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
      361 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
      421 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
      481 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
      541 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
      601 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
      661 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
      721 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
      781 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
      841 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
      901 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
      961 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     1021 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     1081 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     1141 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     1201 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     1261 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     1321 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     1381 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     1441 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     1501 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     1561 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     1621 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     1681 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     1741 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     1801 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     1861 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     1921 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     1981 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2041 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2101 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2221 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     2281 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     2341 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     2401 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     2461 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     2521 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     2581 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     2641 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     2701 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     2761 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     2821 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     2881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3061 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     3121 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     3181 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     3241 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     3301 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3361 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3421 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3481 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3541 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3601 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3661 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3721 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3781 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3841 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3901 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3961 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     4021 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     4081 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     4141 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     4201 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4261 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4321 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4381 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca
     4441 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4501 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4561 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt
     4621 gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
     4681 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca
     4741 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
     4801 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
     4861 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
     4921 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
     4981 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
     5041 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
     5101 cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca
     5161 cgcgtcaagt ggagcaaggc aggtggacag tggatccgga gctaccatgt cccgagccgg
     5221 tagctgggac atggacggcc tgcgagcgga cggaggcgga gctggaggag ctcccgcatc
     5281 aagtagtagc tcaagtgtgg ccgctgccgc ggctagcgga caatgtaggg ggtttttgtc
     5341 agcgcctgtc tttgcgggca cacattccgg gagggccgct gctgcggctg ctgctgctgc
     5401 tgcagccgcg gcggccgcta gtggatttgc ctatcctggg acgagtgagc gcactggttc
     5461 atcatcctca tcttcatcca gtgcggtagt cgccgctcgg ccagaagcac cacctgcaaa
     5521 agagtgtccg gccccgacgc cagctgcagc agcagcggca ccgccttcag ctcctgcggg
     5581 taccggaggc gggatggttt ccaaaggaga ggaggataat atggctataa ttaaagagtt
     5641 tatgcggttc aaggtgcata tggagggatc tgtgaacggt cacgagttcg aaatcgaggg
     5701 cgaaggcgag gggcgaccct atgaagggac acagacggcc aaactgaagg tgaccaaggg
     5761 tggccccctg cctttcgcct gggacatcct gtcaccccag tttatgtacg gctctaaggc
     5821 ttacgttaag caccctgccg atattccgga ctacttgaaa ctgagcttcc ctgaagggtt
     5881 caagtgggaa cgggtaatga attttgagga tggcggtgta gtcacagtta cccaggacag
     5941 ctctcttcag gacggagaat ttatctataa ggttaaactt cggggcacta acttcccatc
     6001 agacggcccc gtgatgcaga agaaaactat gggctgggaa gccagctcag aacgcatgta
     6061 tcccgaggac ggagcactga agggagaaat caagcagcga ctcaagctga aggatggggg
     6121 acattatgat gctgaggtca aaaccaccta taaggccaag aaacccgttc agcttcctgg
     6181 ggcatacaac gtgaatatca aactggatat tacatcccat aacgaagact ataccatcgt
     6241 ggaacagtac gagcgggccg agggcagaca tagcacaggg ggcatggatg aattgtacaa
     6301 ggcggccacg cgtatgaaga tggacaaaaa gactatagtt tggtttagaa gagacctaag
     6361 gattgaggat aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt
     6421 catttggtgt cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat
     6481 gaaacaatca cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt
     6541 aatcaaaacc cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac
     6601 aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa
     6661 ggagaagctg gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga
     6721 accgtgggag atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa
     6781 gaaatgctta gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc
     6841 aataactgca gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa
     6901 tgaggccgag aaaccgagca atgcgttgtt aactagagct tggtctccag gatggagcaa
     6961 tgctgataag ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag
     7021 caagaaagtt gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat
     7081 aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa
     7141 gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta
     7201 ttctcggtat atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct
     7261 tcggtttttc ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac
     7321 cggttatccg ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa
     7381 cagaataaga gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg
     7441 gggaatgaag tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg
     7501 ctggcagtat atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc
     7561 cgcgttacaa ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga
     7621 gcttgcgaga ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact
     7681 caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac
     7741 agctcgtgag ctactagcta aagctatttc aagaacccgt gaagcacaga tcatgatcgg
     7801 agcagcagcc cgggatccac cggtcgccac cggttcaggg agcggatccg gctcagggag
     7861 tgcggccgga actcccacct gcaacatgcg tgacggaggc ggtccaaaaa agaagagaaa
     7921 ggtatgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     7981 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     8041 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     8101 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8161 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8221 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     8281 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8341 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     8401 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8461 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8521 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     8581 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
     8641 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     8701 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     8761 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8821 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8881 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     8941 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     9001 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     9061 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     9121 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     9181 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     9241 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     9301 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     9361 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     9421 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     9481 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     9541 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     9601 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     9661 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     9721 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9781 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9841 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9901 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9961 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    10021 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    10081 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    10141 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10201 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    10261 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10321 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10381 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10441 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10501 ggaacacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10561 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10621 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10681 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10741 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10801 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10861 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10921 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10981 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    11041 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11101 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    11161 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    11221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    11281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    11341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    11401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    11461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    11521 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    11581 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    11641 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    11701 cattaatgca gctgtggaat
//
