LOCUS       sequence-311649-       11741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1464..1529
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1659..1679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2104..2238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2141..2160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2195..2214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2406..3039
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3086..3211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3708..3941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4126..4170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4455..4572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4725..5132
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6335..7828
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7925..7945
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    8010..8598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8063..8083)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8481..8492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8601..8617)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8602..8618)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8708..8941
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8966..8984)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8967..8984)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9274..9293)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9507..9526)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9626..9648
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9686..9704)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9772..9876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9877..9945,9946..10737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10095..10114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10908..11496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11397..11416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11650..11667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(join(11737..11741,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
      361 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
      421 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
      481 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
      541 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
      601 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
      661 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
      721 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
      781 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
      841 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
      901 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
      961 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     1021 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     1081 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     1141 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     1201 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     1261 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     1321 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     1381 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     1441 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     1501 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     1561 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     1621 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     1681 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     1741 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     1801 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     1861 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     1921 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     1981 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2041 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2101 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2221 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     2281 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     2341 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     2401 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     2461 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     2521 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     2581 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     2641 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     2701 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     2761 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     2821 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     2881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3061 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     3121 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     3181 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     3241 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     3301 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3361 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3421 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3481 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3541 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3601 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3661 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3721 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3781 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3841 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3901 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3961 ggaaaactta tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     4021 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     4081 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     4141 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     4201 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4261 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4321 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4381 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca
     4441 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4501 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4561 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt
     4621 gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
     4681 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca
     4741 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
     4801 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
     4861 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
     4921 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
     4981 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
     5041 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
     5101 cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca
     5161 cgcgtcaagt ggagcaaggc aggtggacag tggatccgga gctaccatgt cccgagccgg
     5221 tagctgggac atggacggcc tgcgagcgga cggaggcgga gctggaggag ctcccgcatc
     5281 aagtagtagc tcaagtgtgg ccgctgccgc ggctagcgga caatgtaggg ggtttttgtc
     5341 agcgcctgtc tttgcgggca cacattccgg gagggccgct gctgcggctg ctgctgctgc
     5401 tgcagccgcg gcggcagctg cagctgcggc cgctgccgct agtggatttg cctatcctgg
     5461 gacgagtgag cgcactggtt catcatcctc atcttcatcc agtgcggtag tcgccgctcg
     5521 gccagaagca ccacctgcaa aagagtgtcc ggccccgacg ccagctgcag cagcagcggc
     5581 accgccttca gctcctgcgg gtaccggagg cgggatggtt tccaaaggag aggaggataa
     5641 tatggctata attaaagagt ttatgcggtt caaggtgcat atggagggat ctgtgaacgg
     5701 tcacgagttc gaaatcgagg gcgaaggcga ggggcgaccc tatgaaggga cacagacggc
     5761 caaactgaag gtgaccaagg gtggccccct gcctttcgcc tgggacatcc tgtcacccca
     5821 gtttatgtac ggctctaagg cttacgttaa gcaccctgcc gatattccgg actacttgaa
     5881 actgagcttc cctgaagggt tcaagtggga acgggtaatg aattttgagg atggcggtgt
     5941 agtcacagtt acccaggaca gctctcttca ggacggagaa tttatctata aggttaaact
     6001 tcggggcact aacttcccat cagacggccc cgtgatgcag aagaaaacta tgggctggga
     6061 agccagctca gaacgcatgt atcccgagga cggagcactg aagggagaaa tcaagcagcg
     6121 actcaagctg aaggatgggg gacattatga tgctgaggtc aaaaccacct ataaggccaa
     6181 gaaacccgtt cagcttcctg gggcatacaa cgtgaatatc aaactggata ttacatccca
     6241 taacgaagac tataccatcg tggaacagta cgagcgggcc gagggcagac atagcacagg
     6301 gggcatggat gaattgtaca aggcggccac gcgtatgaag atggacaaaa agactatagt
     6361 ttggtttaga agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga
     6421 aggatctgtt tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg
     6481 aagagcttca agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc
     6541 tcttggatct gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg
     6601 tatccgcgtt accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt
     6661 agttcgggac cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta
     6721 caatggagat ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac
     6781 gagtttcaat tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc
     6841 tcctccttgg cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat
     6901 tgaagaacta gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc
     6961 ttggtctcca ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt
     7021 gatagattat gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc
     7081 gtatctccat ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca
     7141 aattatatgg gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag
     7201 gggaatcggt ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga
     7261 gcaatcgttg ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa
     7321 ggcctggaga caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg
     7381 ggctaccgga tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt
     7441 tcttctcctt ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga
     7501 tttggaatgt gacatccttg gctggcagta tatctctggg agtatccccg atggccacga
     7561 gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta
     7621 cataaggcaa tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg
     7681 ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa
     7741 acccattgta gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg
     7801 tgaagcacag atcatgatcg gagcagcagc ccgggatcca ccggtcgcca ccggttcagg
     7861 gagcggatcc ggctcaggga gtgcggccgg aactcccacc tgcaacatgc gtgacggagg
     7921 cggtccaaaa aagaagagaa aggtatgact gaggccgcga ctctagagtc gacctgcagg
     7981 catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     8041 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     8101 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8161 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8221 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8281 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8341 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8401 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8461 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8521 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8581 tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta
     8641 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8701 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     8761 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8821 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8881 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8941 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     9001 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     9061 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     9121 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     9181 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     9241 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     9301 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     9361 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     9421 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     9481 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9541 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9601 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     9661 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9721 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9781 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9841 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9901 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9961 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10021 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10081 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    10141 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10201 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10261 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10321 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10381 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10441 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10501 atggaggcgg ataaagttgc aggaacactt ctgcgctcgg cccttccggc tggctggttt
    10561 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10621 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10681 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10741 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10801 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11041 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11701 ctccccgcgc gttggccgat tcattaatgc agctgtggaa t
//
