LOCUS       sequence-31165-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Johnson RC, Penzes P, Eipper BA, Mains RE
  TITLE     Isoforms of kalirin, a neuronal Dbl family member, generated through
            use of different 5'- and 3'-ends along with an internal 
            translational initiation site.
  JOURNAL   J Biol Chem. 2000 Jun 23. 275(25):19324-33.
  PUBMED    10777487
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Jun 23. 275(25):19324-33."
COMMENT     Sequence Label: pEAK10-His-Myc-Kal5
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             44..73
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcttgccacc atggcccatc accatcacca tcacaacatg ggggagcaga agctgatctc
       61 tgaggaggac ctgaacatgt ccgtctcttt ccacacgcac accaaagagc tgtggacatg
      121 gatggaagac cttcagaagg aggtgctgga ggatgtctgt gcagactctg tggatgcggt
      181 ccaggaactg atcaagcagt tccagcagca gcagaccgct accctggacg ccactctcaa
      241 cgtcatcaag gaaggcgaag accttatcca gcagctcagg tcagcgcctc cctccctggg
      301 ggagcccacc gaggccaggg actcagctgt atccaacaac aagacgcccc acagtagttc
      361 catcagccat atcgagtctg tcctccagca gctggacgat gctcaggtac agatggagga
      421 gctgtttcac gagcggaaga tcaagctgga catcttcctg cagctgcgca tcttcgaaca
      481 gtacaccatt gaggtcaccg cagagctgga tgcctggaat gaggacttgc tccggcagat
      541 gaatgacttc aacacagagg acctgaccct ggcagagcag cggctacagc gccacacaga
      601 acggaagctt gccatgaaca acatgacatt cgaggtcatc cagcagggcc aggacctgca
      661 ccaatatatc atggaagtcc aagcctcagg aatcgagtta atctgtgaga aggacgttga
      721 cctggcagct caggtgcaag agctactgga atttctccat gagaagcagc acgaactgga
      781 gctcaatgcg gagcagacgc acaagcggct agagcagtgt ctccagctgc ggcacctcca
      841 ggctgaggtc aaacaggttc tgggatggat ccgcaatgga gagtccatgc tcaacgccaa
      901 gcctgg
//
