LOCUS       sequence-311650-       11744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1464..1529
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1659..1679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2104..2238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2141..2160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2195..2214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2406..3039
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3086..3211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3708..3941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4126..4170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4455..4572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4725..5132
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6338..7831
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7928..7948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    8013..8601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8066..8086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8484..8495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8604..8620)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8605..8621)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8711..8944
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8969..8987)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8970..8987)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9277..9296)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9510..9529)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9629..9651
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9689..9707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9775..9879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9880..9948,9949..10740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10098..10117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10911..11499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11400..11419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11653..11670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(join(11740..11744,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
      361 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
      421 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
      481 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
      541 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
      601 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
      661 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
      721 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
      781 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
      841 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
      901 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
      961 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     1021 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     1081 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     1141 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     1201 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     1261 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     1321 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     1381 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     1441 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     1501 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     1561 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     1621 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     1681 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     1741 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     1801 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     1861 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     1921 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     1981 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2041 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2101 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2221 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     2281 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     2341 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     2401 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     2461 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     2521 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     2581 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     2641 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     2701 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     2761 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     2821 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     2881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3061 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     3121 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     3181 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     3241 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     3301 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3361 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3421 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3481 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3541 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3601 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3661 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3721 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3781 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3841 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3901 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3961 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     4021 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     4081 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     4141 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     4201 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4261 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4321 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4381 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca
     4441 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4501 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4561 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt
     4621 gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
     4681 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca
     4741 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
     4801 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
     4861 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
     4921 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
     4981 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
     5041 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
     5101 cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca
     5161 cgcgtcaagt ggagcaaggc aggtggacag tggatccgga gctaccatgt cccgagccgg
     5221 tagctgggac atggacggcc tgcgagcgga cggaggcgga gctggaggag ctcccgcatc
     5281 aagtagtagc tcaagtgtgg ccgctgccgc ggctagcgga caatgtaggg ggtttttgtc
     5341 agcgcctgtc tttgcgggca cacattccgg gagggccgct gctgcggctg ctgctgctgc
     5401 tgcagccgcg gcggcagctg cagctgcggc cgcagctgcc gctagtggat ttgcctatcc
     5461 tgggacgagt gagcgcactg gttcatcatc ctcatcttca tccagtgcgg tagtcgccgc
     5521 tcggccagaa gcaccacctg caaaagagtg tccggccccg acgccagctg cagcagcagc
     5581 ggcaccgcct tcagctcctg cgggtaccgg aggcgggatg gtttccaaag gagaggagga
     5641 taatatggct ataattaaag agtttatgcg gttcaaggtg catatggagg gatctgtgaa
     5701 cggtcacgag ttcgaaatcg agggcgaagg cgaggggcga ccctatgaag ggacacagac
     5761 ggccaaactg aaggtgacca agggtggccc cctgcctttc gcctgggaca tcctgtcacc
     5821 ccagtttatg tacggctcta aggcttacgt taagcaccct gccgatattc cggactactt
     5881 gaaactgagc ttccctgaag ggttcaagtg ggaacgggta atgaattttg aggatggcgg
     5941 tgtagtcaca gttacccagg acagctctct tcaggacgga gaatttatct ataaggttaa
     6001 acttcggggc actaacttcc catcagacgg ccccgtgatg cagaagaaaa ctatgggctg
     6061 ggaagccagc tcagaacgca tgtatcccga ggacggagca ctgaagggag aaatcaagca
     6121 gcgactcaag ctgaaggatg ggggacatta tgatgctgag gtcaaaacca cctataaggc
     6181 caagaaaccc gttcagcttc ctggggcata caacgtgaat atcaaactgg atattacatc
     6241 ccataacgaa gactatacca tcgtggaaca gtacgagcgg gccgagggca gacatagcac
     6301 agggggcatg gatgaattgt acaaggcggc cacgcgtatg aagatggaca aaaagactat
     6361 agtttggttt agaagagacc taaggattga ggataatcct gcattagcag cagctgctca
     6421 cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac agttttatcc
     6481 tggaagagct tcaagatggt ggatgaaaca atcacttgct cacttatctc aatccttgaa
     6541 ggctcttgga tctgacctca ctttaatcaa aacccacaac acgatttcag cgatcttgga
     6601 ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac cacctctatg atcctgtttc
     6661 gttagttcgg gaccataccg taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag
     6721 ctacaatgga gatctattgt atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt
     6781 tacgagtttc aattcttact ggaagaaatg cttagatatg tcgattgaat ccgttatgct
     6841 tcctcctcct tggcggttga tgccaataac tgcagcggct gaagcgattt gggcgtgttc
     6901 gattgaagaa ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt tgttaactag
     6961 agcttggtct ccaggatgga gcaatgctga taagttacta aatgagttca tcgagaagca
     7021 gttgatagat tatgcaaaga acagcaagaa agttgttggg aattctactt cactactttc
     7081 tccgtatctc catttcgggg aaataagcgt cagacacgtt ttccagtgtg cccggatgaa
     7141 acaaattata tgggcaagag ataagaacag tgaaggagaa gaaagtgcag atctttttct
     7201 taggggaatc ggtttaagag agtattctcg gtatatatgt ttcaacttcc cgtttactca
     7261 cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg gatgctgatg ttgataagtt
     7321 caaggcctgg agacaaggca ggaccggtta tccgttggtg gatgccggaa tgagagagct
     7381 ttgggctacc ggatggatgc ataacagaat aagagtgatt gtttcaagct ttgctgtgaa
     7441 gtttcttctc cttccatgga aatggggaat gaagtatttc tgggatacac ttttggatgc
     7501 tgatttggaa tgtgacatcc ttggctggca gtatatctct gggagtatcc ccgatggcca
     7561 cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga
     7621 gtacataagg caatggcttc ccgagcttgc gagattgcca actgaatgga tccatcatcc
     7681 atgggacgct cctttaaccg tactcaaagc ttctggtgtg gaactcggaa caaactatgc
     7741 gaaacccatt gtagacatcg acacagctcg tgagctacta gctaaagcta tttcaagaac
     7801 ccgtgaagca cagatcatga tcggagcagc agcccgggat ccaccggtcg ccaccggttc
     7861 agggagcgga tccggctcag ggagtgcggc cggaactccc acctgcaaca tgcgtgacgg
     7921 aggcggtcca aaaaagaaga gaaaggtatg actgaggccg cgactctaga gtcgacctgc
     7981 aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     8041 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8101 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8161 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8221 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8281 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8341 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8401 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8461 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8521 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8581 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg
     8641 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8701 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     8761 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8821 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8881 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8941 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     9001 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     9061 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     9121 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     9181 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     9241 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     9301 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     9361 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     9421 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     9481 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9541 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9661 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10081 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    10141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10501 tggatggagg cggataaagt tgcaggaaca cttctgcgct cggcccttcc ggctggctgg
    10561 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10981 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11041 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    11101 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    11161 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11221 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11281 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11341 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11401 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11461 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11521 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11581 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11641 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11701 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaat
//
