LOCUS       sequence-311652-       12344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXA13-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..12344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1815..3308
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3405..3425
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3490..4078
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3543..3563)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3961..3972)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4081..4097)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4082..4098)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4188..4421
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4446..4464)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4447..4464)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4754..4773)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4987..5006)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5106..5128
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5166..5184)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5252..5356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5357..5425,5426..6217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5575..5594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6388..6976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6877..6896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7264..7285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7300..7305,7306..7323,7324..7330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7338..7354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7343..7365
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        7621..7950
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7801..7936
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9084..9149
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9279..9299
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9724..9858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9761..9780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9815..9834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10026..10659
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10706..10831
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11328..11561
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11746..11790
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12075..12192
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccggagcta
      481 ccatggagtt gaacaaaaac atggaggggg cggctgcggc tgcagccgcg gcagcggctg
      541 cagcggctgc aggagccggt ggaggaggtt ttccccatcc ggcggcggct gccgcggggg
      601 ggaatttcag tgttgcggca gctgcagcag ctgcagctgc tgccgcagct aaccagtgcc
      661 gcaacctgat ggcgcatcca gcgcctcttg cgccgggggc cgcatcagcg tacagttctg
      721 ctcctgggga agcacccccg tccgcggctg ctgccgcggc agcggcagca gcagccgcgg
      781 ctgcggccgc ggcagctagc tccagtggag gtcccggacc agcgggacct gctggtgcgg
      841 aagccgcgaa gcagtgtagc ccgtgcagcg cggcagctca gagctcatcc ggtcccgccg
      901 ccctcccata cggatacttt ggctcagggt actacccgtg cgcgagaatg ggccctcacc
      961 ccaacgccat aaagtcatgt gcccaacccg cgtcagccgc agcggcagca gcttttgccg
     1021 ataagtacat ggacactgct ggccccgcgg cggaggagtt cagtagcagg gcgaaggagg
     1081 gtaccggagg cgggatggtt tccaaaggag aggaggataa tatggctata attaaagagt
     1141 ttatgcggtt caaggtgcat atggagggat ctgtgaacgg tcacgagttc gaaatcgagg
     1201 gcgaaggcga ggggcgaccc tatgaaggga cacagacggc caaactgaag gtgaccaagg
     1261 gtggccccct gcctttcgcc tgggacatcc tgtcacccca gtttatgtac ggctctaagg
     1321 cttacgttaa gcaccctgcc gatattccgg actacttgaa actgagcttc cctgaagggt
     1381 tcaagtggga acgggtaatg aattttgagg atggcggtgt agtcacagtt acccaggaca
     1441 gctctcttca ggacggagaa tttatctata aggttaaact tcggggcact aacttcccat
     1501 cagacggccc cgtgatgcag aagaaaacta tgggctggga agccagctca gaacgcatgt
     1561 atcccgagga cggagcactg aagggagaaa tcaagcagcg actcaagctg aaggatgggg
     1621 gacattatga tgctgaggtc aaaaccacct ataaggccaa gaaacccgtt cagcttcctg
     1681 gggcatacaa cgtgaatatc aaactggata ttacatccca taacgaagac tataccatcg
     1741 tggaacagta cgagcgggcc gagggcagac atagcacagg gggcatggat gaattgtaca
     1801 aggcggccac gcgtatgaag atggacaaaa agactatagt ttggtttaga agagacctaa
     1861 ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt tttcctgtct
     1921 tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca agatggtgga
     1981 tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct gacctcactt
     2041 taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt accggtgcta
     2101 caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac cataccgtaa
     2161 aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat ctattgtatg
     2221 aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat tcttactgga
     2281 agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg cggttgatgc
     2341 caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta gggctggaga
     2401 atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca ggatggagca
     2461 atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat gcaaagaaca
     2521 gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat ttcggggaaa
     2581 taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg gcaagagata
     2641 agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt ttaagagagt
     2701 attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg ttgagtcatc
     2761 ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga caaggcagga
     2821 ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga tggatgcata
     2881 acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat
     2941 ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt gacatccttg
     3001 gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc ttggacaatc
     3061 ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa tggcttcccg
     3121 agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct ttaaccgtac
     3181 tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta gacatcgaca
     3241 cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag atcatgatcg
     3301 gagcagcagc ccgggatcca ccggtcgcca ccggttcagg gagcggatcc ggctcaggga
     3361 gtgcggccgg aactcccacc tgcaacatgc gtgacggagg cggtccaaaa aagaagagaa
     3421 aggtatgact gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     3481 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3541 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3601 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3661 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3721 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3781 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3841 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3901 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3961 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4021 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     4081 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     4141 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4201 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4261 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4321 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4381 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     4441 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     4501 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     4561 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     4621 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     4681 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     4741 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     4801 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     4861 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     4921 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     4981 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5041 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5101 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5161 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5221 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5281 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5341 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5401 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5461 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5521 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5581 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5641 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5701 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5761 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5821 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5881 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5941 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6001 aggaacactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6061 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6121 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6181 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7021 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7081 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7141 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7201 tcattaatgc agctgtggaa tgtgtgtcms ykaskggaaa gcgggcagtg agcgcaacgc
     7261 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7321 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctcagttgt
     7381 gtaaamgacm ttttttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7441 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7501 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7561 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat
     7621 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7681 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7741 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7801 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7861 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7921 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
     7981 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     8041 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     8101 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     8161 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     8221 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
     8281 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     8341 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     8401 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     8461 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
     8521 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
     8581 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     8641 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     8701 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     8761 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     8821 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     8881 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     8941 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     9001 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     9061 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     9121 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     9181 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     9241 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     9301 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     9361 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     9421 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     9481 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     9541 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     9601 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     9661 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     9721 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9841 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     9901 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     9961 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10021 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
    10081 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
    10141 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
    10201 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
    10261 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
    10321 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
    10381 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
    10441 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
    10501 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10561 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10621 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
    10681 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    10741 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
    10801 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
    10861 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
    10921 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    10981 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    11041 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    11101 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    11161 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    11221 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    11281 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    11341 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    11401 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    11461 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    11521 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    11581 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    11641 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    11701 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    11761 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    11821 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    11881 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    11941 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    12001 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca
    12061 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    12121 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    12181 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt
    12241 gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
    12301 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgca
//
