LOCUS       sequence-311653-       11966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1836..3329
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3426..3446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3511..4099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3564..3584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3982..3993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4102..4118)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4103..4119)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4209..4442
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4467..4485)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4468..4485)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4775..4794)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5008..5027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5127..5149
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5187..5205)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5273..5377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5378..5446,5447..6238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5596..5615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6409..6997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6898..6917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7151..7168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7238..7258)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7243..7572
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7423..7558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8706..8771
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8901..8921
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9346..9480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9383..9402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9437..9456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9648..10281
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10328..10453
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10950..11183
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11368..11412
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11697..11814
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccggagcta
      481 ccatggagtt gaacaaaaac atggaggggg cggctgcggc tgcagccgcg gcagcggctg
      541 cagcggctgc aggagccggt ggaggaggtt ttccccatcc ggcggcggct gccgcggggg
      601 ggaatttcag tgttgcggca gctgcagcag ctgcagctgc tgccgcagct aaccagtgcc
      661 gcaacctgat ggcgcatcca gcgcctcttg cgccgggggc cgcatcagcg tacagttctg
      721 ctcctgggga agcacccccg tccgcggctg ctgccgcggc agcggcagca gcagccgcgg
      781 ctgcggcagc tgcagctgcg gccgctgccg cggcagctag ctccagtgga ggtcccggac
      841 cagcgggacc tgctggtgcg gaagccgcga agcagtgtag cccgtgcagc gcggcagctc
      901 agagctcatc cggtcccgcc gccctcccat acggatactt tggctcaggg tactacccgt
      961 gcgcgagaat gggccctcac cccaacgcca taaagtcatg tgcccaaccc gcgtcagccg
     1021 cagcggcagc agcttttgcc gataagtaca tggacactgc tggccccgcg gcggaggagt
     1081 tcagtagcag ggcgaaggag ggtaccggag gcgggatggt ttccaaagga gaggaggata
     1141 atatggctat aattaaagag tttatgcggt tcaaggtgca tatggaggga tctgtgaacg
     1201 gtcacgagtt cgaaatcgag ggcgaaggcg aggggcgacc ctatgaaggg acacagacgg
     1261 ccaaactgaa ggtgaccaag ggtggccccc tgcctttcgc ctgggacatc ctgtcacccc
     1321 agtttatgta cggctctaag gcttacgtta agcaccctgc cgatattccg gactacttga
     1381 aactgagctt ccctgaaggg ttcaagtggg aacgggtaat gaattttgag gatggcggtg
     1441 tagtcacagt tacccaggac agctctcttc aggacggaga atttatctat aaggttaaac
     1501 ttcggggcac taacttccca tcagacggcc ccgtgatgca gaagaaaact atgggctggg
     1561 aagccagctc agaacgcatg tatcccgagg acggagcact gaagggagaa atcaagcagc
     1621 gactcaagct gaaggatggg ggacattatg atgctgaggt caaaaccacc tataaggcca
     1681 agaaacccgt tcagcttcct ggggcataca acgtgaatat caaactggat attacatccc
     1741 ataacgaaga ctataccatc gtggaacagt acgagcgggc cgagggcaga catagcacag
     1801 ggggcatgga tgaattgtac aaggcggcca cgcgtatgaa gatggacaaa aagactatag
     1861 tttggtttag aagagaccta aggattgagg ataatcctgc attagcagca gctgctcacg
     1921 aaggatctgt ttttcctgtc ttcatttggt gtcctgaaga agaaggacag ttttatcctg
     1981 gaagagcttc aagatggtgg atgaaacaat cacttgctca cttatctcaa tccttgaagg
     2041 ctcttggatc tgacctcact ttaatcaaaa cccacaacac gatttcagcg atcttggatt
     2101 gtatccgcgt taccggtgct acaaaagtcg tctttaacca cctctatgat cctgtttcgt
     2161 tagttcggga ccataccgta aaggagaagc tggtggaacg tgggatctct gtgcaaagct
     2221 acaatggaga tctattgtat gaaccgtggg agatatactg cgaaaagggc aaacctttta
     2281 cgagtttcaa ttcttactgg aagaaatgct tagatatgtc gattgaatcc gttatgcttc
     2341 ctcctccttg gcggttgatg ccaataactg cagcggctga agcgatttgg gcgtgttcga
     2401 ttgaagaact agggctggag aatgaggccg agaaaccgag caatgcgttg ttaactagag
     2461 cttggtctcc aggatggagc aatgctgata agttactaaa tgagttcatc gagaagcagt
     2521 tgatagatta tgcaaagaac agcaagaaag ttgttgggaa ttctacttca ctactttctc
     2581 cgtatctcca tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc cggatgaaac
     2641 aaattatatg ggcaagagat aagaacagtg aaggagaaga aagtgcagat ctttttctta
     2701 ggggaatcgg tttaagagag tattctcggt atatatgttt caacttcccg tttactcacg
     2761 agcaatcgtt gttgagtcat cttcggtttt tcccttggga tgctgatgtt gataagttca
     2821 aggcctggag acaaggcagg accggttatc cgttggtgga tgccggaatg agagagcttt
     2881 gggctaccgg atggatgcat aacagaataa gagtgattgt ttcaagcttt gctgtgaagt
     2941 ttcttctcct tccatggaaa tggggaatga agtatttctg ggatacactt ttggatgctg
     3001 atttggaatg tgacatcctt ggctggcagt atatctctgg gagtatcccc gatggccacg
     3061 agcttgatcg cttggacaat cccgcgttac aaggcgccaa atatgaccca gaaggtgagt
     3121 acataaggca atggcttccc gagcttgcga gattgccaac tgaatggatc catcatccat
     3181 gggacgctcc tttaaccgta ctcaaagctt ctggtgtgga actcggaaca aactatgcga
     3241 aacccattgt agacatcgac acagctcgtg agctactagc taaagctatt tcaagaaccc
     3301 gtgaagcaca gatcatgatc ggagcagcag cccgggatcc accggtcgcc accggttcag
     3361 ggagcggatc cggctcaggg agtgcggccg gaactcccac ctgcaacatg cgtgacggag
     3421 gcggtccaaa aaagaagaga aaggtatgac tgaggccgcg actctagagt cgacctgcag
     3481 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3541 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3601 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3661 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3721 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3781 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3841 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3901 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3961 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4021 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4081 ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt
     4141 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4201 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4261 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4321 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4381 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4441 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     4501 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     4561 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     4621 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     4681 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     4741 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     4801 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     4861 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     4921 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     4981 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5041 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5101 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5161 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5221 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5281 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5341 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5401 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5461 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5521 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5581 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5641 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5701 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5761 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5821 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5881 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5941 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6001 gatggaggcg gataaagttg caggaacact tctgcgctcg gcccttccgg ctggctggtt
     6061 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6121 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6181 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6541 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6781 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7021 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7081 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7141 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7201 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     7261 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7321 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7381 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7441 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7501 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7561 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     7621 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     7681 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     7741 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     7801 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     7861 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     7921 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     7981 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     8041 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     8101 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     8161 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     8221 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     8281 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     8341 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     8401 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     8461 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     8521 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     8581 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     8641 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     8701 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     8761 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     8821 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     8881 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     8941 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     9001 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     9061 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     9121 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     9181 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     9241 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     9301 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     9361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9481 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9541 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     9601 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     9661 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     9721 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     9781 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     9841 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     9901 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     9961 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
    10021 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
    10081 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10141 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10201 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10261 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    10321 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    10381 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    10441 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    10501 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    10561 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    10621 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    10681 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    10741 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
    10801 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    10861 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    10921 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    10981 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    11041 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    11101 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    11161 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    11221 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    11281 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    11341 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    11401 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    11461 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    11521 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    11581 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    11641 attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat ccacaatttt
    11701 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
    11761 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
    11821 tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga attcctgcag
    11881 ccccgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
    11941 cctgtaggtt tggcaagcta gctgca
//
