LOCUS       sequence-311654-       11620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto RUNX2 IDR  with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-RUNX2-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      425..434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1490..2983
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3080..3100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3165..3753
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3218..3238)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3636..3647)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3756..3772)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3757..3773)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3863..4096
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4121..4139)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4122..4139)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4429..4448)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4662..4681)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4781..4803
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4841..4859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4927..5031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5032..5100,5101..5892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5250..5269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6063..6651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6552..6571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6805..6822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6892..6912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6897..7226
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7077..7212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8360..8425
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8555..8575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9000..9134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9037..9056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9091..9110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9302..9935
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9982..10107
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10604..10837
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11022..11066
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11351..11468
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggaa gagaggccac
      421 tagtgccacc atggcatcaa acagcctctt cagcacagtg acaccatgtc agcaaaactt
      481 cttttgggat ccgagcacca gccggcgctt cagccccccc tccagcagcc tgcagcccgg
      541 caaaatgagc gacgtgagcc cggtggtggc tgcgcaacag cagcagcaac agcagcagca
      601 gcaacagcag cagcagcagc agcaacagca gcagcagcag caggaggcgg cggcggccgc
      661 tgcggcggcg gcggcggctg cggcggcggc agctgcagtg ccccggttac ggccgcccca
      721 cgacaaccgc accatggtgg agacgtacgg ggtctggctc gtcgggtcca tggtttccaa
      781 aggagaggag gataatatgg ctataattaa agagtttatg cggttcaagg tgcatatgga
      841 gggatctgtg aacggtcacg agttcgaaat cgagggcgaa ggcgaggggc gaccctatga
      901 agggacacag acggccaaac tgaaggtgac caagggtggc cccctgcctt tcgcctggga
      961 catcctgtca ccccagttta tgtacggctc taaggcttac gttaagcacc ctgccgatat
     1021 tccggactac ttgaaactga gcttccctga agggttcaag tgggaacggg taatgaattt
     1081 tgaggatggc ggtgtagtca cagttaccca ggacagctct cttcaggacg gagaatttat
     1141 ctataaggtt aaacttcggg gcactaactt cccatcagac ggccccgtga tgcagaagaa
     1201 aactatgggc tgggaagcca gctcagaacg catgtatccc gaggacggag cactgaaggg
     1261 agaaatcaag cagcgactca agctgaagga tgggggacat tatgatgctg aggtcaaaac
     1321 cacctataag gccaagaaac ccgttcagct tcctggggca tacaacgtga atatcaaact
     1381 ggatattaca tcccataacg aagactatac catcgtggaa cagtacgagc gggccgaggg
     1441 cagacatagc acagggggca tggatgaatt gtacaaggcg gccacgcgta tgaagatgga
     1501 caaaaagact atagtttggt ttagaagaga cctaaggatt gaggataatc ctgcattagc
     1561 agcagctgct cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg
     1621 acagttttat cctggaagag cttcaagatg gtggatgaaa caatcacttg ctcacttatc
     1681 tcaatccttg aaggctcttg gatctgacct cactttaatc aaaacccaca acacgatttc
     1741 agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta accacctcta
     1801 tgatcctgtt tcgttagttc gggaccatac cgtaaaggag aagctggtgg aacgtgggat
     1861 ctctgtgcaa agctacaatg gagatctatt gtatgaaccg tgggagatat actgcgaaaa
     1921 gggcaaacct tttacgagtt tcaattctta ctggaagaaa tgcttagata tgtcgattga
     1981 atccgttatg cttcctcctc cttggcggtt gatgccaata actgcagcgg ctgaagcgat
     2041 ttgggcgtgt tcgattgaag aactagggct ggagaatgag gccgagaaac cgagcaatgc
     2101 gttgttaact agagcttggt ctccaggatg gagcaatgct gataagttac taaatgagtt
     2161 catcgagaag cagttgatag attatgcaaa gaacagcaag aaagttgttg ggaattctac
     2221 ttcactactt tctccgtatc tccatttcgg ggaaataagc gtcagacacg ttttccagtg
     2281 tgcccggatg aaacaaatta tatgggcaag agataagaac agtgaaggag aagaaagtgc
     2341 agatcttttt cttaggggaa tcggtttaag agagtattct cggtatatat gtttcaactt
     2401 cccgtttact cacgagcaat cgttgttgag tcatcttcgg tttttccctt gggatgctga
     2461 tgttgataag ttcaaggcct ggagacaagg caggaccggt tatccgttgg tggatgccgg
     2521 aatgagagag ctttgggcta ccggatggat gcataacaga ataagagtga ttgtttcaag
     2581 ctttgctgtg aagtttcttc tccttccatg gaaatgggga atgaagtatt tctgggatac
     2641 acttttggat gctgatttgg aatgtgacat ccttggctgg cagtatatct ctgggagtat
     2701 ccccgatggc cacgagcttg atcgcttgga caatcccgcg ttacaaggcg ccaaatatga
     2761 cccagaaggt gagtacataa ggcaatggct tcccgagctt gcgagattgc caactgaatg
     2821 gatccatcat ccatgggacg ctcctttaac cgtactcaaa gcttctggtg tggaactcgg
     2881 aacaaactat gcgaaaccca ttgtagacat cgacacagct cgtgagctac tagctaaagc
     2941 tatttcaaga acccgtgaag cacagatcat gatcggagca gcagcccggg atccaccggt
     3001 cgccaccggt tcagggagcg gatccggctc agggagtgcg gccggaactc ccacctgcaa
     3061 catgcgtgac ggaggcggtc caaaaaagaa gagaaaggta tgactgaggc cgcgactcta
     3121 gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt
     3181 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3241 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3301 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3361 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3421 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3481 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3541 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     3601 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3661 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3721 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt
     3781 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     3841 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     3901 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3961 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4021 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4081 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     4141 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     4201 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     4261 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     4321 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     4381 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     4441 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     4501 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     4561 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     4621 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
     4681 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4741 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4801 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4861 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5641 caattaatag actggatgga ggcggataaa gttgcaggaa cacttctgcg ctcggccctt
     5701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6781 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6841 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     6901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7081 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7141 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7201 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
     7261 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     7321 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     7381 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
     7441 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
     7501 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
     7561 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
     7621 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
     7681 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
     7741 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
     7801 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
     7861 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
     7921 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
     7981 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
     8041 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
     8101 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
     8161 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
     8221 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
     8281 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     8341 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     8401 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     8461 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     8521 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     8581 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     8641 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     8701 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     8761 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     8821 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     8881 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     8941 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     9001 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9061 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9121 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     9181 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     9241 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     9301 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     9361 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     9421 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     9481 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     9541 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     9601 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     9661 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     9721 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     9781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9901 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     9961 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    10021 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    10081 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    10141 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    10201 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    10261 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    10321 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    10381 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    10441 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    10501 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10561 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10621 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    10681 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    10741 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    10801 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    10861 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    10921 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    10981 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    11041 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    11101 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    11161 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    11221 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
    11281 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat
    11341 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11401 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11461 aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata
    11521 tcgaattcct gcagccccga taaaataaaa gattttattt agtctccaga aaaagggggg
    11581 aatgaaagac cccacctgta ggtttggcaa gctagctgca
//
