LOCUS       sequence-311655-        2440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  low phosphate yibDp induction of Griffithsin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Decker JS, Menacho-Melgar R, Lynch MD
  TITLE     Low-Cost, Large-Scale Production of the Anti-viral Lectin 
            Griffithsin.
  JOURNAL   Front Bioeng Biotechnol. 2020 Aug 21;8:1020. doi: 
            10.3389/fbioe.2020.01020. eCollection 2020.
  PUBMED    32974328
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2020-08-21"; volume: "8:1020. doi"; 
            pages: " 10.3389/fbioe.2020.01020. eCollection 2020"
COMMENT     Sequence Label: pHCKAn-yibDp-GRFT
FEATURES             Location/Qualifiers
     source          1..2440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      274..283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      535..606
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      622..649
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      720..751
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        752..854
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             855..1670
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(928..947)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1691..1718
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1730..2317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1810..1829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2339..2368)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gtgcgtaatt gtgctgatct cttatatagc tgctctcatt atctctctac cctgaagtga
       61 ctctctcacc tgtaaaaata atatctcaca ggcttaatag tttcttaata caaagcctgt
      121 aaaacgtcag gataacttct gtgtaggagg ataatctatg tctttaaccc accgtaaatt
      181 tggtggatcc ggtggttcac cttttagtgg attatcgtct attgcggtgc gctctggctc
      241 gtacttagat gcaatcatca ttgatggagt acaccatggc ggctccggcg gcaacctgtc
      301 gcctactttc acttttggca gtggggagta tattagcaat atgaccatcc gctcgggtga
      361 ttatattgac aatatcagct ttgagacgaa tatggggcgt cgtttcggcc catacggagg
      421 gtcgggtggt tcggctaata cgctgtccaa tgtaaaggta atccaaatta atggctccgc
      481 tggcgactat cttgattcgt tggacattta ttatgaacaa tattgaccag gcatcaaata
      541 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      601 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatagacga
      661 attctctaga tatcgctcaa tactgaccat ttaaatcata cctgacctcc atagcagaaa
      721 gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt tccaactttc
      781 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      841 ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
      901 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      961 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
     1021 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcaggcta aactggctga
     1081 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     1141 tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa tatcctgatt
     1201 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1261 tttgtaattg tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1321 tgaataacgg tttggttggt gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     1381 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
     1441 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1501 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     1561 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     1621 ctgatatgaa taaattgcag tttcacttga tgctcgatga gtttttctga gggcccaaat
     1681 gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt ttccataggc
     1741 tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga
     1801 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1861 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1921 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1981 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2041 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2101 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2161 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag
     2221 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2281 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga ttttctaccg
     2341 aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag
     2401 tctgaggctc gtcctgaatg atatcaagct tgaattcgtt
//
