LOCUS       sequence-311656-        2406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  low phosphate phoBp induction of Griffithsin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Decker JS, Menacho-Melgar R, Lynch MD
  TITLE     Low-Cost, Large-Scale Production of the Anti-viral Lectin 
            Griffithsin.
  JOURNAL   Front Bioeng Biotechnol. 2020 Aug 21;8:1020. doi: 
            10.3389/fbioe.2020.01020. eCollection 2020.
  PUBMED    32974328
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2020-08-21"; volume: "8:1020. doi"; 
            pages: " 10.3389/fbioe.2020.01020. eCollection 2020"
COMMENT     Sequence Label: pHCKAn-phoBp-GRFT
FEATURES             Location/Qualifiers
     source          1..2406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      240..249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      501..572
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      588..615
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      686..717
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        718..820
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             821..1636
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(894..913)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1657..1684
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1696..2283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1776..1795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2305..2334)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gccacggaaa tcaataacct gaagatatgt gcgacgagct tttcataaat ctgtcataaa
       61 tctgacgcat aatgacgtcg cattaatgat cgcaacctat ttatttgtag gaggataatc
      121 tatatgtctt taacccaccg taaatttggt ggatccggtg gttcaccttt tagtggatta
      181 tcgtctattg cggtgcgctc tggctcgtac ttagatgcaa tcatcattga tggagtacac
      241 catggcggct ccggcggcaa cctgtcgcct actttcactt ttggcagtgg ggagtatatt
      301 agcaatatga ccatccgctc gggtgattat attgacaata tcagctttga gacgaatatg
      361 gggcgtcgtt tcggcccata cggagggtcg ggtggttcgg ctaatacgct gtccaatgta
      421 aaggtaatcc aaattaatgg ctccgctggc gactatcttg attcgttgga catttattat
      481 gaacaatatt gaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      541 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      601 gtgggccttt ctgcgtttat agacgaattc tctagatatc gctcaatact gaccatttaa
      661 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga
      721 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      781 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga
      841 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      901 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
      961 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     1021 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     1081 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt
     1141 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     1201 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt
     1261 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga
     1321 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     1381 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     1441 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     1501 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     1561 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct
     1621 cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc
     1681 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg
     1741 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1801 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1861 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1921 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1981 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2041 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2101 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2161 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2281 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag
     2341 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa
     2401 ttcgtt
//
