LOCUS       sequence-311657-        2395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  low phosphate phoAp induction of Griffithsin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Decker JS, Menacho-Melgar R, Lynch MD
  TITLE     Low-Cost, Large-Scale Production of the Anti-viral Lectin 
            Griffithsin.
  JOURNAL   Front Bioeng Biotechnol. 2020 Aug 21;8:1020. doi: 
            10.3389/fbioe.2020.01020. eCollection 2020.
  PUBMED    32974328
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2020-08-21"; volume: "8:1020. doi"; 
            pages: " 10.3389/fbioe.2020.01020. eCollection 2020"
COMMENT     Sequence Label: pHCKAn-phoAp-GRFT
FEATURES             Location/Qualifiers
     source          1..2395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      229..238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      490..561
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      577..604
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      675..706
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        707..809
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             810..1625
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(883..902)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1646..1673
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1685..2272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1765..1784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2294..2323)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gttaatcttt tcaacagctg tcataaagtt gtcacggccg agacttatag tcgctttgtt
       61 tttatttttt aatgtatttg tatctagaga ttaaagagga gaatactaga tgatgtcttt
      121 aacccaccgt aaatttggtg gatccggtgg ttcacctttt agtggattat cgtctattgc
      181 ggtgcgctct ggctcgtact tagatgcaat catcattgat ggagtacacc atggcggctc
      241 cggcggcaac ctgtcgccta ctttcacttt tggcagtggg gagtatatta gcaatatgac
      301 catccgctcg ggtgattata ttgacaatat cagctttgag acgaatatgg ggcgtcgttt
      361 cggcccatac ggagggtcgg gtggttcggc taatacgctg tccaatgtaa aggtaatcca
      421 aattaatggc tccgctggcg actatcttga ttcgttggac atttattatg aacaatattg
      481 accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      541 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      601 tgcgtttata gacgaattct ctagatatcg ctcaatactg accatttaaa tcatacctga
      661 cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa
      721 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
      781 cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct
      841 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
      901 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
      961 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     1021 ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     1081 ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag
     1141 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     1201 tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc
     1261 aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta
     1321 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg
     1381 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     1441 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     1501 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     1561 atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt
     1621 tctgagggcc caaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg
     1681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga
     1741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2101 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2161 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2221 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2281 tttgattttc taccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgcagt
     2341 ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat tcgtt
//
